We narrowed to 21,813 results for: Sis

1. pDONR223-NEK9

   Plasmid

   #23459

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   NEK9 (NEK9 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   1MM

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pDONR223-NEK8

   Plasmid

   #23418

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   NEK8 (NEK8 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pDONR223-NEK5

   Plasmid

   #23434

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   NEK5 (NEK5 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-PGK-EGFP-ALDH1A3-Neo

   Plasmid

   #189744

   Purpose

   The construct was used to express GFP tagged human ALDH1A3 in mammalian cells for the analysis of the subcellular location of this protein.

   Depositor

   Robert Sobol

   Article

   Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

   Insert

   ALDH1A3 (ALDH1A3 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223-TSSK3

   Plasmid

   #23553

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   TSSK3 (TSSK3 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pDONR223-NEK3

   Plasmid

   #23821

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   NEK3 (NEK3 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Venus

   Plasmid

   #105805

   Purpose

   Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Venus, cleavable by TEV. Useful for protein interaction analysis by FRET.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   ORF1: 3C-Cerulean; ORF2: TEV-Venus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSLPB3B-mAID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

   Plasmid

   #187963

   Purpose

   mAID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Inserts

   AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
   osTIR1
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS linker and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBABE-RPS14(WT)-Myc

   Plasmid

   #122235

   Purpose

   expresses RPS14 protein with a Myc tag in mammalian cells (puro resistance)

   Depositor

   Gerardo Ferbeyre

   Article

   Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

   Insert

   RPS14 (RPS14 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   vector's LTR

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPHAGE-CR-NENF-C-TAP

    Plasmid

    #128511

    Purpose

    Lentiviral constitutive expression of CRISPR-resistant NENF with c-terminal FLAG and HA tag.

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    NENF (NENF Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Synonymous silent mutations to F81, Y82, G83 and …

    Promoter

    CMV

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223-TSSK1

    Plasmid

    #23740

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    TSSK1 (TSSK1B Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pEAHISMRSA0482

    Plasmid

    #96968

    Purpose

    expression of methicillin-resistant Staphylococcus aureus orf 0482

    Depositor

    Huanting Liu, James Naismith

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    MRSA ORF0482
    

    Use

    Tags

    N-ter TEV protease cleavable 6His

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223-NEK4

    Plasmid

    #23497

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    NEK4 (NEK4 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Short isoform, only goes to aa632

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pDONR223-NEK11

    Plasmid

    #23816

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    NEK11 (NEK11 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pDONR223-STK17B

    Plasmid

    #23721

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    STK17B (STK17B Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pDONR223-TSSK2

    Plasmid

    #23397

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    TSSK2 (TSSK2 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    1MM

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Amber

    Plasmid

    #105806

    Purpose

    Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Amber, cleavable by TEV. Control for protein interaction analysis by FRET.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: 3C-Cerulean; ORF2: TEV-Amber

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223-NEK10

    Plasmid

    #23437

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    NEK10 (NEK10 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pCDH-3_Flag-YTHDF2-D-MUT (nonsense mutation of sgYTHDF2-1)

    Plasmid

    #232947

    Purpose

    Expresses the domain-mutant YTHDF2 with synonymous mutations for resistance against sgYTHDF2-1-mediated knockdown

    Depositor

    Jianjun Chen

    Article

    Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

    Insert

    YTHDF2-D-MUT (YTHDF2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    5 site mutation (Y418A+D422A+W432A+W486A+W491A), …

    Promoter

    Available since

    May 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDH-3_Flag-YTHDF2 (nonsense mutation of sgYTHDF2-1)

    Plasmid

    #232945

    Purpose

    Expresses YTHDF2 with synonymous mutations rendering it resistant to sgYTHDF2-1-mediated knockdown

    Depositor

    Jianjun Chen

    Article

    Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

    Insert

    YTHDF2 (YTHDF2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    synonymous mutation

    Promoter

    Available since

    May 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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