We narrowed to 15,102 results for: EGF;
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Plasmid#236363PurposeOverexpression of endAsCas12a in human cellsDepositorInsertendAsCas12a
UseCRISPR and LentiviralMutationE174R, S542R, K548RAvailable SinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-HyperdAsCas12a-EGFP-NLS-3xFlag
Plasmid#236362PurposeOverexpression of HyperdAsCas12a in human cellsDepositorInsertHyperdAsCas12a
UseCRISPR and LentiviralMutationE174R, D256R, E321R, E371RAvailable SinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dAsCas12a-EGFP-NLS-3xFlag
Plasmid#236361PurposeOverexpression of dAsCas12a in human cellsDepositorInsertdAsCas12a
UseCRISPR and LentiviralMutationWTAvailable SinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
LSM041-3 SFFV-mCherry-SGc(stem-1(3xPP7))cSG-EGFP
Plasmid#233408PurposeLIDAR reporter RNA with PCP binding site instead of MCP; mCherry as marker, EGFP as outputDepositorInsertmCherry-SGc(stem-1(3xPP7))cSG-EGFP
ExpressionMammalianMutationWTPromoterSFFVAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
APL0038 Ac5-mCherry-SGc(stem-1(3xMS2))cSG-EGFP
Plasmid#233410PurposeAC5 promoter driven LIDAR reporter RNA for drosophila cell expression (mCherry as marker, EGFP as output)DepositorInsertmCherry-SGc(stem-1(3xMS2))cSG-EGFP
ExpressionMammalianMutationWTPromoterAc5Available SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (I224A/L227A; deltaLIR3)
Plasmid#223775PurposeExpression of recombinant protein for purificationDepositorInsertBCL2L13 soluble part (BCL2L13 Human)
TagsEGFP-TEV-GSTExpressionBacterialMutationI224A/L227A; deletion of LIR3Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (I307A/V310A; deltaLIR4)
Plasmid#223776PurposeExpression of recombinant protein for purificationDepositorInsertBCL2L13 soluble part (BCL2L13 Human)
TagsEGFP-TEV-GSTExpressionBacterialMutationI307A/V310A; deletion of LIR4Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (Y213A/I216A; deltaLIR2)
Plasmid#223783PurposeExpression of recombinant protein for purificationDepositorInsertBCL2L13 soluble part (BCL2L13 Human)
TagsEGFP-TEV-GSTExpressionBacterialMutationY213A/I216A; deletion of LIR2Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (W276A/I279A; deltaLIR1)
Plasmid#223746PurposeExpression of recombinant protein for purificationDepositorInsertBCL2L13 soluble part (BCL2L13 Human)
TagsEGFP-TEV-GSTExpressionBacterialMutationW276A/I279A; deletion of LIR1Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_NIX(1-182aa)-EGFP-TEV-GST (W36A/L39A; deltaLIR)
Plasmid#223748PurposeExpression of recombinant protein for purificationDepositorInsertNIX (BNIP3L) soluble part (BNIP3L Human)
TagsEGFP-TEV-GSTExpressionBacterialMutationW36A/L39A; deletion of LIRAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_FUNDC1(1-50aa)-EGFP-TEV-GST (Y18A/L21A; deltaLIR)
Plasmid#223750PurposeExpression of recombinant protein for purificationDepositorInsertFUNDC1 soluble part (FUNDC1 Human)
TagsEGFP-TEV-GSTExpressionBacterialMutationexpresses aa 1-50, with Y18A/L21A; deletion of LIRAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-EGFP-Aubergine 4R-K (R11K/R13K/R15K/R17K) (HK121)
Plasmid#206433PurposeAubergine expression in Schneider cells for imagingDepositorAvailable SinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro cTnT CCND2-T2A-ChloC-P2A-eGFP
Plasmid#179840Purposedonor plasmid for cardiac-specific CCND2-T2A-Chloc-P2A-eGFP expression in human cellsDepositorInsertCCND2 (CCND2 Human)
UseCRISPR and TALEN; Donor plasmidTagschloride-conducting ChR (ChloC) and eGFPExpressionMammalianPromoterTroponin T promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC
Plasmid#202717PurposeMammalian co-expression of membrane-bound Pink Flamindo and soluble bPAC for cAMP perturbation and imagingDepositorArticleInsertsMyr-Pink Flamindo
EGFP-bPAC
UseLentiviralTags10xHis and mycExpressionMammalianPromoterCMVAvailable SinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-inv[Talpha1-Dre-pA]-lox-Lyn-EGFP
Plasmid#196876PurposeNeuron-specific expression of LynGFP reporter. Used in combination with Talpha1-iCre-pA plasmidsDepositorInsertLynEGFP
UseCre/Lox; Dre/roxExpressionMammalianPromoterQuimeric CAGAvailable SinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-CIM1mut-F961A_S
Plasmid#147514PurposeInsect Expression of CeAIN2_254-400-DmGW182_635-1384-CIM1-F961ADepositorInsertCeAIN2_254-400-DmGW182_635-1384-CIM1-F961A (gw Nematode, Human)
ExpressionInsectMutationDeletion of 2 AA (SD) at position 378-379 compare…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut_R
Plasmid#147388PurposeInsect Expression of CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mutDepositorInsertCeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut (gw Nematode, Fly)
ExpressionInsectMutationDeletion of 2 AA (SD) at position 378-379 compare…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-P-GLmut-F961A_R
Plasmid#147391PurposeInsect Expression of CeAIN2_254-400-DmGW182_635-1384-P-GLmut-F961ADepositorInsertCeAIN2_254-400-DmGW182_635-1384-P-GLmut-F961A (gw Fly)
ExpressionInsectMutationDeletion of 2 AA (SD) at position 378-379 compare…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1mut-CIM2mut_R
Plasmid#147396PurposeInsect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mutDepositorInsertCeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut (TNRC6C Nematode, Human)
ExpressionInsectMutationDeletion of 2 AA (SD) at position 378-379 compare…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-CIM1-mut_R
Plasmid#147385PurposeInsect Expression of CeAIN2_254-400-DmGW182_635-1384-CIM1-mutDepositorInsertCeAIN2_254-400-DmGW182_635-1384-CIM1-mut (gw Nematode, Fly)
ExpressionInsectMutationDeletion of 2 AA (SD) at position 378-379 compare…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
PL452-PTPRO-LA-p2a-EGFP-hGHpA-PGK-neoR-PTPRO-RA
Plasmid#191689PurposeFor knocking p2a-EGFP into PTPRO locusDepositorInsertPTPRO homology arms and p2a-EGFP
ExpressionMammalianMutationWTAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1_1-1605-del1079-1110-RNFtoAAA-shRNAres_W
Plasmid#147886PurposeMammalian Expression of HsNot1iso1_1-1605-del1097-1110-RNFtoAAA-shRNAresDepositorInsertHsNot1iso1_1-1605-del1097-1110-RNFtoAAA-shRNAres (CNOT1 Human)
ExpressionMammalianMutation1 silent mutation compared to the sequence of iso…Available SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAres_W
Plasmid#147887PurposeMammalian Expression of HsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAresDepositorInsertHsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAres (CNOT1 Human)
ExpressionMammalianMutation1 silent mutation compared to the sequence of iso…Available SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP1205-Ai227 (TICF-nls-EGFP-ICF-tdTomato-WPRE)
Plasmid#181761Purposetargeting vectorDepositorInsertnls-tagged EGFP and tdTomato
ExpressionMammalianAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EGFP-PolB-(K35A/K68A/K72A)-PAMmut-Hygro
Plasmid#176089PurposeEGFP fused the N-terminus of POLB containing the mutations Lys35Ala, Lys68Ala, and Lys72Ala, a mutation in the PAM site used by POLB gRNA1 & a hygromycin resistance cassetteDepositorInsertPolB (POLB Human)
UseLentiviralTagsEGFPExpressionMammalianMutationMutations in Lys35 to Ala, Lys68 to Ala, and Lys7…PromoterCMVAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
PylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)
Plasmid#174528PurposeDual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiencyDepositorInsertPylS
TagsFlagExpressionMammalianAvailable SinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E12: attB5r-RU5u-His6-eGFP-nostop-attB1r
Plasmid#162954PurposeGateway attB5r/attB1r entry clone for generation of N-terminal eGFP-tagged fusion proteinsDepositorInsertHis6-Green fluorescent protein fusion tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEM230 - pEXP[Pmlc-1 | ceGFP(2xNLS) | cbr-tbb-2 UTR]
Plasmid#159794PurposeFluorescent co-injection marker to identify extrachromosomal arrays in C. elegansDepositorInsertpEXP[Pmlc-1 | ceGFP(2xNLS) | cbr-tbb-2 UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide2
Plasmid#118159PurposeCRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA2 against the EGFP CDSDepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterEF1a core and U6Available SinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide3
Plasmid#118160PurposeCRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA3 against the EGFP CDSDepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterEF1a core and U6Available SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-deGFP Y35TAG D193TAG-6xHis-T500
Plasmid#92227PurposeExpression of deGFP mutant Y35TAG D193TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertdeGFP Y35TAG D193TAG
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationResidues Y35 D193 were mutated into TAG (Amber) s…Promoterp70aAvailable SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWPT-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherry
Plasmid#49227PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianPromoterEF1-shortAvailable SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCBh_NLS_hfCas13X(Cas13X_M17YY)_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA
Plasmid#190033Purposevector for encoding a human codon-optimized High-fidelity Cas13X (hfCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP and mCherry driven by SV40 promotersDepositorInserthumanized hfCas13X
ExpressionMammalianMutationY672A,Y676APromoterCBh, SV40, hU6Available SinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0493 SFFV-mCherry-SGc(GAPDH.s1)cSG-EGFP-(3xMS2) (FLP-IN)
Plasmid#191156PurposeExpression of a sensor for GAPDH 3' UTR in mammalian cells; mCherry marker, EGFP output, three MS2 sequences in 3' UTRDepositorInsertmCherry:GAPDH.s1:EGFP-3xMS2
ExpressionMammalianMutationWTPromoterSFFVAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-MTS-TagBFP-P2AT2A-EGFP-NLS-P2AT2A-mCherry-PTS1
Plasmid#87829PurposeHigh-efficient mammalian expression vector for co-expression of BFP-, EGFP- and mCherry-tagged proteins using P2AT2A. MTS, NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsMTS
NLS
PTS1
ExpressionMammalianPromoterCMV promoter, tetracycline operatorAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZR043_Lenti-U6-sgEGFR-MS2-PP7-hPGK-PCP-p65-HSF1-Puro
Plasmid#180267PurposeLentiviral expression vector for CRISPRa-SAM with EGFR targeting sgRNADepositorInsertEGFR sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMini-CMV-NLS-dead R-IscB-NLS_T2A_EGFP (Y67X)_LNMA intron
Plasmid#246435PurposeExpresses inactive EGFP (Y67X) in mammalian cells for trans-splicing assessments. LMNA intron is inserted into EGFP CDS.DepositorInsertsO.gue IscB with dead mutations (D60A, H269A) and delta-TID
EGFP
TagsSV40 NLS, Thosea asigna virus 2A peptide, and nuc…ExpressionMammalianMutationchanged Aspartic Acid 60 to Alanine, changed Hist…PromoterCMVAvailable SinceOct. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDH-MND-OsTIR1(F74G)-P2A-EGFP-AID2-EF1a-RFP
Plasmid#232800PurposeHelper plasmid to trigger auxin-inducible-degradation of targeted proteins.DepositorInsertOsTIR1 F74G EGFP-AID
UseLentiviralAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
VEE-mScarlet3-IRES-E3-NSs-L*-EGFP-IRES-srIκBα-Smad7-SOCS1
Plasmid#242412PurposeSelf-amplifying RNA (saRNA) construct expresses dsRNA pathway inhibitors (E3, NSs, L*) and inflammatory signaling inhibitors, srIκBα, Smad7, and SOCS1.DepositorInsertsmScarlet3
IRES-E3-NSs-L*-EGFP
IRES-srIκBα-Smad7-SOCS1
UseMouse Targeting; Self-amplifying rnaExpressionMammalianAvailable SinceSept. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
PSB-CMV-Puro ArfGAP1_ALPS1_ALPS2-mKate2-3NLS-P2A-EGFP-Sec61B
Plasmid#250913PurposeDual color expression of mKate2-3NLS tagged human ARFGAP1 ALPS1-2 and EGFP tagged human Sec 61B in mammalian cell linesDepositorTagsEGFP on Sec61b and mKate2-3xNLS on ArfGAP1ExpressionMammalianMutationARFGAP1 amino acids 192–304 onlyPromoterCMVAvailable SinceFeb. 17, 2026AvailabilityAcademic Institutions and Nonprofits only