We narrowed to 18,458 results for: Met

1. pclbw-eGFPomp25

   Plasmid

   #58424

   Purpose

   Mammalian expression of GFP targeted to the mitochondrial outer membrane

   Depositor

   David Chan

   Article

   Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

   Insert

   eGFP-omp25 (Synj2bp Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   contains aa's 109-145 of mouse omp25

   Promoter

   CMV/b-actin

   Available Since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

2. TNF-SBP-EGFP_puromycin

   Plasmid

   #65284

   Purpose

   TNF reporter only (no hook) (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   TNF (TNF Human)
   

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSFDuet-1_mNeongreen-Etanercept

   Plasmid

   #182420

   Purpose

   Bacterial expression of mNeongreen-Etanercept fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   mNeongreen-Etanercept (TNFRSF1B Human)
   

   Tags

   mNeongreen

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-mNeptune2

   Plasmid

   #51309

   Purpose

   Expresses far-red fluorescent protein mNeptune2 in mammalian cells

   Depositor

   Michael Lin

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   mNeptune2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. mRuby2-Tubulin-C-18

   Plasmid

   #55915

   Purpose

   Localization: Microtubules, Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   Tubulin (TUBA1B Human)
   

   Tags

   mRuby2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

6. Lenti-LbCpf1-Blast

   Plasmid

   #84751

   Purpose

   Expresses human codon-optimized LbCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

   Insert

   LbCpf1
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS-3xHA (C terminal on insert)

   Expression

   Mammalian

   Promoter

   EFS-NS

   Available Since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pB-CAG-FLEX-F14F15-BFP-(mCherry)'

   Plasmid

   #201831

   Purpose

   piggyBac transposon plasmid for making Cre and Flpo reporter cell lines using piggyBac transposase

   Depositor

   Ian Wickersham

   Article

   Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Inserts

   CAG
   BFP
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)

   Plasmid

   #47550

   Purpose

   Cas9 + sgRNA plasmid that is targeted to a genomic site near the ttTi5605 Mos1 insertion allele

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

   Inserts

   Cas9
   ttTi5605 sgRNA
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Worm

   Mutation

   Codon optimized and with synthetic introns for C.…

   Promoter

   U6 and eef-1A.1 (eft-3)

   Available Since

   Sept. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. mTagRFP-T-Clathrin-15

   Plasmid

   #58005

   Purpose

   Localization: Clathrin Vesicles, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   Clathrin (CLTB Human)
   

   Tags

   mTagRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 31, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. APLF-c001

    Plasmid

    #173077

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    APLF (APLF Human)
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    K360-V448, codon optimized

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCyRFP1-RhoA

    Plasmid

    #84358

    Purpose

    fusion protein of RhoA, FRET donor

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mCyRFP1-RhoA (RHOA Synthetic, Human)
    

    Tags

    mCyRFP1 N terminal fusion to RhoA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223_HcRed

    Plasmid

    #25892

    Purpose

    Gateway entry vector containing HcRed.

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    hcRed
    

    Use

    Gateway entry vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti_CamKIIa_mKate2

    Plasmid

    #96942

    Purpose

    To label mature excitatory iPSC-derived human neurons

    Depositor

    Ricardo Dolmetsch, Oleksandr Shcheglovitov

    Article

    Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

    Insert

    far-red fluorescent protein
    

    Use

    Lentiviral

    Mutation

    E87D (please see depositor comments below)

    Promoter

    CaMKIIa

    Available Since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pECE M2-SNX17 WT

    Plasmid

    #69811

    Purpose

    Expresses M2-SNX17 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    Sorting nexin-17 (SNX17 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    SV40 early promoter

    Available Since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. 5HT6-YC3.60

    Plasmid

    #48339

    Purpose

    FRET reporter for Ca2+ inside primary cilia

    Depositor

    Takanari Inoue

    Article

    Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

    Insert

    5-hydroxytriptamine receptor isoform 6 (Htr6 Mouse)
    

    Tags

    YC3.60

    Expression

    Mammalian

    Available Since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

16. 2xHP1bCD_W42A-IFP2.0C

    Plasmid

    #215751

    Purpose

    Expresses binding-deficient W42A mutant of 2xHP1beta chromo domain fused to C-terminal part of IFP2.0 as negative control for H3K9me2/3 detection in BiAD sensors

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    2xHP1bCD_W42A-IFP2.0C
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMXS-IRES-Blast coSHMT2

    Plasmid

    #106299

    Purpose

    Expresses SHMT2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    serine hydroxymethyltransferase 2 (SHMT2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Codon optimized

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-SNAP-CRY2-VHH(GFP)

    Plasmid

    #58370

    Purpose

    For use in light-induced inactivation of GFP fused protein through the interaction with CIB1-MP

    Depositor

    Won Do Heo

    Article

    Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

    Insert

    CRY2[1-498] (CRY2 Mustard Weed)
    

    Tags

    SNAP and vhhGFP4

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDL14

    Plasmid

    #106901

    Purpose

    pDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent manner

    Depositor

    Sergey Sineoky

    Article

    Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

    Inserts

    gam
    bet
    mutS
    lacI
    rhaR
    rhaS
    

    Expression

    Bacterial

    Mutation

    Lysine 622 was replaced by Alanine

    Available Since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. MacroH2A1.1-c002

    Plasmid

    #173087

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    MACROH2A1 (MACROH2A1 Human)
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    Q162-N369, codon optimized

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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