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tdTomato-Gamma-Tubulin-17

Plasmid

#58099

Purpose

Localization: Centrosomes, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Gamma-Tubulin (TUBG1 Human)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pmCherry-CIBN-CreC

Plasmid

#26889

Purpose

Expresses C-terminal fragment of Cre fused to CIBN for blue light-induced activation of DNA recombination when used with CRY2-CreN. Also expresses mCherry.

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CIBN-CreC

Expression

Mammalian

Mutation

aa 1-170 of CIB1, aa 106-343 of Cre

Promoter

CMV

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-eArch 3.0-EYFP

Plasmid

#35516

Purpose

AAV expression of CaMKIIa-driven Arch 3.0 for optical inhibition.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

Arch3.0-EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

Enhanced trafficking signal and ER export signal …

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pCDH_Str-KDEL_ST-SBP-EGFP

Plasmid

#65267

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)

Use

Lentiviral

Tags

EGFP and Streptavidin Binding Protein (SBP)

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast SLC5A7

Plasmid

#218539

Purpose

human SLC5A7 cDNA

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

SLC5A7 (SLC5A7 Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast SLC44A1

Plasmid

#218538

Purpose

human SLC44A1 cDNA

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

SLC44A1 (SLC44A1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-LaminB1-10

Plasmid

#58020

Purpose

Localization: Nuclear Envelope, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

LaminB1 (LMNB1 Human)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9

Plasmid

#84240

Purpose

Expresses GA-inducible VPR-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

GAI-tagBFP-Sp dCas9
GID1-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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mCardinal-Lysosomes-20

Plasmid

#56166

Purpose

Localization: Lysosome Membrane, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

LAMP1

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
Tom20-CR

Plasmid

#171461

Purpose

Expresses Tom20-CR, an anchor protein of iCMM/NiCMM, in mammalian cells

Depositor

Takafumi Miyamoto

Article

Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

Insert

Tom20 (1-34 aa)-ECFP-FRB (2021-2113 aa)

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS1

Plasmid

#170852

Purpose

AAV backbone for transgene expression in neurons under the control of the hSyn1 promoter.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Gamma-Tubulin-17

Plasmid

#58017

Purpose

Localization: Centrosomes, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Gamma-Tubulin (TUBG1 Human)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCry2PHR-mCh-RhoA

Plasmid

#42958

Purpose

Expresses optogenetically controlled RhoA

Depositor

David Schaffer

Article

Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

Insert

Cry2PHR-mCherry-RhoA (RHOA Mustard Weed, Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pExodus CMV.Trex2

Plasmid

#40210

Purpose

Mammalian expression of codon-optimized Trex2.

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi:

Insert

Three prime repair exonuclease 2 (Trex2 Mouse)

Expression

Bacterial and Mammalian

Promoter

CMV and T7

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pEB2-mCherry2-L

Plasmid

#104003

Purpose

Plasmid encoding mCherry2-L

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mCherry2-L

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to DsRed (MSKGEE NNLA IIKEF… V…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-mNeongreen-DARPinOFF7

Plasmid

#182415

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen-DARPinOFF7 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-DARPinOFF7

Tags

Vesicle Nucleating peptide (VNp) & mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Actin Binding Domain Tension Sensor

Plasmid

#215368

Purpose

Expresses Actin Binding Domain Tension Sensor (ABDTS), composed of Ftractin-Linker-TSMod-Linker-Ftractin. TSMod is the tension sensor module containing mTFP1 and mVenus.

Depositor

Brenton Hoffman

Article

Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

Actin Binding Domain Tension Sensor

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRb1/Cre

Plasmid

#89647

Purpose

Expresses an Rb1-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Rb1 (Rb1 Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mNeptune2

Plasmid

#51309

Purpose

Expresses far-red fluorescent protein mNeptune2 in mammalian cells

Depositor

Michael Lin

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

mNeptune2

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2014

Availability

Academic Institutions and Nonprofits only

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pclbw-eGFPomp25

Plasmid

#58424

Purpose

Mammalian expression of GFP targeted to the mitochondrial outer membrane

Depositor

David Chan

Article

Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

Insert

eGFP-omp25 (Synj2bp Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

contains aa's 109-145 of mouse omp25

Promoter

CMV/b-actin

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only