We narrowed to 14,039 results for: TIM

1. lenti EF1a-FLAG-dCas9-VPR

   Plasmid

   #114195

   Purpose

   Lentivirus compatible dCas9-VPR construct driven by the EF1a promoter

   Depositor

   Jeremy Day

   Article

   Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

   Insert

   dCas9-VPR
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMTL5

   Plasmid

   #127967

   Purpose

   DHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus

   Depositor

   Martin Kampmann

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   AAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
   

   Use

   TALEN

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CaMKIIa-DIO eHcKCR1 3.0-oScarlett-KV2.1

   Plasmid

   #195198

   Purpose

   all-optical silencing method

   Depositor

   Karl Deisseroth

   Article

   Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi

   Insert

   eHcKCR1 3.0-oScarlet-Kv2.1
   

   Use

   AAV

   Promoter

   CaMKIIa

   Available Since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLPCX mito Grx1-roGFP2

   Plasmid

   #64977

   Purpose

   Mammalian expression of mitochondrial Grx1-roGFP2 (retroviral vector)

   Depositor

   Tobias Dick

   Article

   Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Use

   Retroviral

   Tags

   roGFP2

   Expression

   Mammalian

   Mutation

   mitochondrial targeting sequence

   Available Since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pN251

   Plasmid

   #188471

   Purpose

   Lentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)

   Depositor

   Le Cong

   Article

   Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

   Insert

   enCas12a-HF (gift from Dr. Keith Joung)
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA

   Plasmid

   #137830

   Purpose

   MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal L190K mutant TEV protease, M cleavage sequence, and tTA transcription factor

   Depositor

   Joshua Leonard

   Article

   Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

   Insert

   FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA
   

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Mutated Leucine 71 to Lysine in CTEVp fragment

   Promoter

   CMV

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS-Hsp70-Cas9

   Plasmid

   #46294

   Purpose

   A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.

   Depositor

   Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

   Article

   Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

   Insert

   codon optimized Cas9
   

   Use

   CRISPR

   Tags

   3x Flag

   Expression

   Insect

   Mutation

   Silent change from C to A at bp 834 of insert

   Promoter

   Hsp70

   Available Since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLPCX cyto Grx1-roGFP2

   Plasmid

   #64975

   Purpose

   Mammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)

   Depositor

   Tobias Dick

   Article

   Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Use

   Retroviral

   Tags

   roGFP2

   Expression

   Mammalian

   Available Since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. piggyBAC-H2B FR-MQV

   Plasmid

   #194079

   Purpose

   Expresses nuclear-localized FR-MQV in mammalian cells

   Depositor

   Ralph Jimenez

   Article

   Mukherjee et al Biochemistry. 2020 Oct 6;59(39):3669-368

   Insert

   FR-MQV
   

   Tags

   Histone H2B

   Expression

   Mammalian

   Mutation

   Methionine 42 to Glutamine, Cystiene 159 to Valin…

   Promoter

   CMV7

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB_EF1a-DjCas13d-msfGFP-Blast

    Plasmid

    #226008

    Purpose

    DjCas13d protein for RNA targeting in piggyBac backbone

    Depositor

    Silvana Konermann

    Article

    Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

    Insert

    DjCas13d RNA-targeting nuclease
    

    Use

    CRISPR

    Tags

    msfGFP

    Expression

    Mammalian

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-EFS-MCS-WPRE

    Plasmid

    #163362

    Purpose

    The empty pLenti backbone with multiple cloning site that allows shuttling between pMY and pMX vectors

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. 4xnrUAS:MLS-KillerRed in pTol2pA2-acrys-EGFP-

    Plasmid

    #115516

    Purpose

    Plasmid driving expression of membrane bound KillerRed fluorophore under UAS response element, with a green lens reporter

    Depositor

    Marco Morsch

    Article

    Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

    Insert

    MLS-KillerRed
    

    Use

    Gateway cloning

    Tags

    MLS

    Promoter

    UAS

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Puro_siKD

    Plasmid

    #86695

    Purpose

    Tetracycline inducible expression of shRNA targeted to AAVS1 locus

    Depositor

    Ludovic Vallier

    Article

    Bertero et al Development. 2016 Dec 1;143(23):4405-441

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    H1 TO

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDule-Acd82

    Plasmid

    #174099

    Purpose

    Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

    Depositor

    Ryan Mehl

    Article

    Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

    Insert

    Acridone 82 tRNA synthetase and tRNA
    

    Expression

    Bacterial

    Mutation

    N311S, C313G, V366A, W382T

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. mEGFP-HRas

    Plasmid

    #18662

    Depositor

    Karel Svoboda

    Article

    Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

    Insert

    H-Ras (HRAS Human)
    

    Tags

    monomeric EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBI-CMV1-mir144[3072 nt]~451-CMV2-mir1

    Plasmid

    #164682

    Purpose

    Express miR-144[3072 nt]~451 and miR-1-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    miR-144[3072 nt]~451 (MIR144 Human)
    mir-1-1 (MIR1-1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHT-6xR.ads-PxylA-DI

    Plasmid

    #47386

    Purpose

    Expresses ads under Pgrac promoter (inducible by IPTG) and expresses dxs and idi under PxylA promoter (inducible by xylose) in bacteria

    Depositor

    Heng-Phon Too

    Article

    Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

    Inserts

    ads
    dxs
    idi
    

    Tags

    6xR and His

    Expression

    Bacterial

    Promoter

    Pgrac and PxylA

    Available Since

    Oct. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. CRY2(L348F)-CreN

    Plasmid

    #75368

    Purpose

    Expresses mCherry tag, then IRES, then Arabidopsis CRY2 (with L348F mutation) fused to N-terminal fragment of Cre DNA recombinase

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Tags

    Cre recombinase, residues 19-104

    Expression

    Mammalian

    Mutation

    L348F

    Promoter

    CMV

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMY-IRES-eGFP

    Plasmid

    #163361

    Purpose

    Retroviral backbone for in-frame cloning of a gene of interest and an IRES separated GFP marker

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLNCX2-mEGFP-CHMP2B

    Plasmid

    #115329

    Purpose

    expresses mEGFP-CHMP2B in mammalian cells

    Depositor

    Sanford Simon

    Article

    Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

    Insert

    CHMP2B (CHMP2B Human)
    

    Use

    Retroviral

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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