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We narrowed to 10,047 results for: pET

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Showing: 3861 - 3880 of 10047 results

  1. pET21-10XHis-GST-HRV-Her1-dL5-Her1

    Plasmid
    #73217
    Purpose
    Bacterial expression vector for Z1907-dL5(E52D)-Z1907 affibody fusion protein (AFA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP, AffiFAP)
    Depositor
    Marcel Bruchez
    Publication
    Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44
    Insert
    10XHis-GST-HRV-Her1-dL5-Her1 (EGFR Schistosoma japonicum, Synthetic, Human)
    Tags
    10XHis-GST-HRV and The Her1 Affibody and dL5 FAP …
    Expression
    Bacterial
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    T7
    Available since
    March 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73217-plasmid-map-sequence-id-140443

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  2. pET28a-HIS-TagGFP2-TEV-GGG-ELP(40nm)-Cys

    Plasmid
    #90474
    Purpose
    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal TagGFP2 and TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine
    Depositor
    Hermann Gaub
    Insert
    TagGFP2-TEV-GGG-ELP(40nm)-Cys
    Tags
    Cysteine and Sortase-Tag
    Expression
    Bacterial
    Promoter
    T7
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    90474-plasmid-map-sequence-id-181091

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  3. pCJ198

    Plasmid
    #162671
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating F495I mutation.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    F495I; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162671-plasmid-map-sequence-id-321447

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  4. pCJ196

    Plasmid
    #162669
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating catalytic mutation, S225A
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    S225A; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162669-plasmid-map-sequence-id-321445

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  5. pCJ204

    Plasmid
    #162677
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224H and C529F mutations.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    C224H, C529F; codon optimized for expression in E…
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162677-plasmid-map-sequence-id-321439

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  6. pCJ201

    Plasmid
    #162674
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224W and C529SS mutations.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    C224W, C529SS; codon optimized for expression in …
    Promoter
    T7/lac
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162674-plasmid-map-sequence-id-320830

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  7. pETM11-SUMO3- sfGFP-KSR1-CA3 (N-KSR)

    Plasmid
    #217767
    Purpose
    Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)
    Depositor
    Paula Nunes-Hasler
    Publication
    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do
    Insert
    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    Use
    Protein purification (6xhis tag, tev site, sumo3 …
    Tags
    sfGFP
    Expression
    Bacterial
    Mutation
    CA3 domain aa 317-400
    Promoter
    T7 promoter
    Available since
    June 10, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    217767-plasmid-map-sequence-id-435788

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  8. pETM11-SUMO3-KSR1-CA3-sfGFP (C-KSR)

    Plasmid
    #217766
    Purpose
    Fluorescent reporter for ceramide (for bacterial expression and purification)
    Depositor
    Paula Nunes-Hasler
    Publication
    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do
    Insert
    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    Use
    Protein purification (6xhis tag, tev site, sumo3 …
    Tags
    sfGFP
    Expression
    Bacterial
    Mutation
    CA3 domain aa 317-400 plus vector-based linker LE…
    Promoter
    T7 promoter
    Available since
    June 10, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    217766-plasmid-map-sequence-id-435787

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  9. Affibody EGFR:1907.BirA*-pET301/NT-DEST

    Plasmid
    #174691
    Purpose
    Plasmid encoding for the bacterial expression of a bispecific coding for the anti-EGFR affibody ZEGFR:1907 fused to the mutated form of BirA enzyme, BirA*
    Depositor
    Jean Gariepy
    Publication
    Alwash et al ACS Pharmacol Transl Sci. 2022 Feb 1;5(2
    Insert
    Anti-EGFR affibody ZEGFR:1907 fused to mutated form of BirA, BirA*
    Tags
    HIS tag
    Expression
    Bacterial
    Mutation
    R118G mutation in BirA
    Promoter
    T7 promoter
    Available since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    174691-plasmid-map-sequence-id-347065

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  10. pET29b-AviTag-eGFP-dspB(E184Q)-6xHis

    Plasmid
    #175784
    Purpose
    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.
    Depositor
    Mark Nitz
    Publication
    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12
    Insert
    Dispersin B
    Tags
    AviTag, Hexahistidine tag, and eGFP
    Expression
    Bacterial
    Mutation
    E184Q
    Promoter
    T7
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    175784-plasmid-map-sequence-id-345284

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  13. pET30a(+)-T7-C-NanoLuc

    Plasmid
    #261423
    Purpose
    Expresses T7 with C-terminal NanoLuc (+ HisTag in E. coli), kanamycin 50 µg/ml, T7 = RNA polymerase (required for RNA transcription)
    Depositor
    Andrei Lupas
    Insert
    T7 (T7p07 )
    Tags
    Fluorescent protein (FP) and HIS-tag: C-terminal …
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    261423-plasmid-map-sequence-id-524999

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  14. pET30a(+)-CK-C-SuperfolderBFP

    Plasmid
    #261420
    Purpose
    Expresses CK with C-terminal SuperfolderBFP (+ HisTag in E. coli), kanamycin 50 µg/ml, CK = creatine kinase (required for ATP and GTP regeneration, removes -P from creatine phosphate)
    Depositor
    Andrei Lupas
    Insert
    CK (CKM )
    Tags
    Fluorescent protein (FP) and HIS-tag: C-terminal …
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    261420-plasmid-map-sequence-id-524993

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  15. pET30a(+)-AK-C-mCerulean

    Plasmid
    #261419
    Purpose
    Expresses AK with C-terminal mCerulean2.D3 (+ HisTag in E. coli), kanamycin 50 µg/ml, AK = adenylate kinase (required for ATP regeneration 2 ADP ⇌ ATP + AMP)
    Depositor
    Andrei Lupas
    Insert
    AK (AK1 )
    Tags
    Fluorescent protein (FP) and HIS-tag: C-terminal …
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    261419-plasmid-map-sequence-id-524992

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  17. pET28a-PPiase

    Plasmid
    #259959
    Purpose
    Expression vector for producing E. coli PPiase, a component of the PURE cell-free transcription/translation system, in E. coli.
    Depositor
    Jonathan Calles
    Insert
    PPiase
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 6, 2026
    Availability
    Industry, Academic Institutions, and Nonprofits
    259959-plasmid-map-sequence-id-522865

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  18. pET28a-MTF

    Plasmid
    #259956
    Purpose
    Expression vector for producing E. coli methionyl-tRNA transformylase, a component of the PURE cell-free transcription/translation system, in E. coli.
    Depositor
    Jonathan Calles
    Insert
    MTF (fmt E. coli (Ec))
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 6, 2026
    Availability
    Industry, Academic Institutions, and Nonprofits
    259956-plasmid-map-sequence-id-522882

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  19. pET28a-HisRS

    Plasmid
    #259950
    Purpose
    Expression vector for producing E. coli histidyl-tRNA synthetase (HisRS), a component of the PURE cell-free transcription/translation system, in E. coli.
    Depositor
    Jonathan Calles
    Insert
    HisRS
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 6, 2026
    Availability
    Industry, Academic Institutions, and Nonprofits
    259950-plasmid-map-sequence-id-522881

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  20. pET28a-EFTu

    Plasmid
    #259946
    Purpose
    Expression vector for producing E. coli elongation factor Tu (EF-Tu), a component of the PURE cell-free transcription/translation system, in E. coli.
    Depositor
    Jonathan Calles
    Insert
    EF-Tu
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 6, 2026
    Availability
    Industry, Academic Institutions, and Nonprofits
    259946-plasmid-map-sequence-id-522880

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Showing: 3861 - 3880 of 10047 results