We narrowed to 6,266 results for: wus

1. pDONR221 Ub-NLRP1 L1218

   Plasmid

   #166829

   Purpose

   pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1218 N-terminus (no stop)

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Ubiquitin

   Expression

   Mutation

   Promoter

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDestTol2-actinb-Hs.KCNJ13-IRES-EGFP

   Plasmid

   #164945

   Purpose

   Tol2 construct: actinb (actb2) promoter driven human KCNJ13 and EGFP”. This will reflect the linkage of KCNJ13 and EGFP

   Depositor

   GuangJun Zhang

   Article

   Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

   Insert

   KCNJ13 (KCNJ13 Human)
   

   Use

   Tol2 transposon destination vector

   Tags

   IRES-EGFP

   Expression

   Mutation

   Stop codon deleted

   Promoter

   actinb (actb2) promoter

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDestTol2-actinb-kcnj13-Q153H-EGFP

   Plasmid

   #164943

   Purpose

   Tol2 construct: actinb (actb2) promoter driven zebrafish loss-of-functionkcnj13 mutant, Q153H and M154I

   Depositor

   GuangJun Zhang

   Article

   Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

   Insert

   kcnj13 (kcnj13 Zebrafish)
   

   Use

   Tol2 transposon destination vector

   Tags

   EGFP

   Expression

   Mutation

   Stop codon deleted; Q153H; M154I

   Promoter

   actinb (actb2) promoter

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDestTol2-actinb-kcnj13-T131A-EGFP

   Plasmid

   #164942

   Purpose

   Tol2 construct: actinb (actb2) promoter driven zebrafish loss-of-function kcnj13 mutant, T131A and G133R

   Depositor

   GuangJun Zhang

   Article

   Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

   Insert

   kcnj13 (kcnj13 Zebrafish)
   

   Use

   Tol2 transposon destination vector

   Tags

   EGFP

   Expression

   Mutation

   Stop codon deleted; T131A; G133R

   Promoter

   actinb (actb2) promoter

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDestTol2-actinb-kcnj1b-IRES-EGFP

   Plasmid

   #164946

   Purpose

   Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj1b and EGFP

   Depositor

   GuangJun Zhang

   Article

   Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

   Insert

   kcnj1b (kcnj1b Zebrafish)
   

   Use

   Tol2 transposon destination vector

   Tags

   IRES-EGFP

   Expression

   Mutation

   Promoter

   actinb (actb2) promoter

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. HBT-CPK10ac-Flag

   Plasmid

   #107427

   Purpose

   For Arabidopsis mesophyll protoplast transient expression assay

   Depositor

   Jen Sheen

   Article

   Liu et al Nature. 2017 May 18;545(7654):311-316. d

   Insert

   CPK10 (CDPK1 Mustard Weed)
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. HBT-CPK10-Flag

   Plasmid

   #107428

   Purpose

   For Arabidopsis mesophyll protoplast transient expression assay

   Depositor

   Jen Sheen

   Article

   Liu et al Nature. 2017 May 18;545(7654):311-316. d

   Insert

   CPK10 (CDPK1 Mustard Weed)
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HBT-CPK30ac-Flag

   Plasmid

   #107429

   Purpose

   For Arabidopsis mesophyll protoplast transient expression assay

   Depositor

   Jen Sheen

   Article

   Liu et al Nature. 2017 May 18;545(7654):311-316. d

   Insert

   CPK30 (CPK30 Mustard Weed)
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. HBT-CPK30-Flag

   Plasmid

   #107430

   Purpose

   For Arabidopsis mesophyll protoplast transient expression assay

   Depositor

   Jen Sheen

   Article

   Liu et al Nature. 2017 May 18;545(7654):311-316. d

   Insert

   CPK30 (CPK30 Mustard Weed)
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HBT-CPK32ac-Flag

    Plasmid

    #107431

    Purpose

    For Arabidopsis mesophyll protoplast transient expression assay

    Depositor

    Jen Sheen

    Article

    Liu et al Nature. 2017 May 18;545(7654):311-316. d

    Insert

    CPK32 (CPK32 Mustard Weed)
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-XYLT2-sgRNA

    Plasmid

    #154862

    Purpose

    Lentiviral expression of Cas9 and sgRNA targeting XYLT2

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    XYLT2 sgRNA (XYLT2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. ARB366

    Plasmid

    #124049

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to KRAB domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::KRAB-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. ARB367

    Plasmid

    #124050

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to VPR domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::VPR-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAVsc.U6-sgFah.Intron9.CMV/CB-EGFP

    Plasmid

    #121509

    Purpose

    Expresses sgRNA targeting mouse Fah intron 9 (sgFah.Intron9).

    Depositor

    Guangping Gao

    Article

    Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

    Insert

    sgFah.intron9
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. EF1a_MYC Glu39Ala_P2A_Hygro_Barcode

    Plasmid

    #120512

    Purpose

    Barcoded lentiviral vector to express MYC Glu39Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC Glu39Ala (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Point mutation changing Glutamic Acid to Alanine …

    Promoter

    EF1a

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. EF1a_KLF8_P2A_Hygro_Barcode

    Plasmid

    #120494

    Purpose

    Barcoded lentiviral vector to express KLF8 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    KLF8 (KLF8 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAVsc.U6-sgIdua.CMV/CB-EGFP

    Plasmid

    #121511

    Purpose

    Expresses sgRNA targeting mouse Idua intron 9 (sgIdua).

    Depositor

    Guangping Gao

    Article

    Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

    Insert

    sgIdua
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAVsc.U6-sgAspa.CMV/CB-EGFP

    Plasmid

    #121510

    Purpose

    Expresses sgRNA targeting mouse Aspa intron 2 (sgAspa).

    Depositor

    Guangping Gao

    Article

    Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

    Insert

    sgAspa
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lenti_Neo_WT_ZFP64_CR

    Plasmid

    #118694

    Purpose

    Express sgZFP64e2 resistant cDNA

    Depositor

    Christopher Vakoc

    Article

    Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

    Insert

    ZFP64 (ZFP64 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    sgZFP64e2 targeting site was synonymous mutated

    Promoter

    EF1a

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti_Neo_ZFP64_N_3XFLAG

    Plasmid

    #118700

    Purpose

    Express 3xflag tagged ZFP64

    Depositor

    Christopher Vakoc

    Article

    Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

    Insert

    ZFP64 (ZFP64 Human)
    

    Use

    Lentiviral

    Tags

    3XFLAG was fused to the N terminal of ZFP64 cDNA

    Expression

    Mutation

    Promoter

    EF1A

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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