164,577 results

1. pHCMV_COCVmut_G

   Plasmid

   #207262

   Purpose

   Mammalian expression of a mutant Cocal virus G protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   COCVmut_G
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K47A/Y209A/R354A; mammalian codon optimization

   Promoter

   CMV promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p-eGFP-beta 2 CP

   Plasmid

   #13298

   Depositor

   John Cooper

   Article

   Schafer et al J Cell Biol. 1998 Dec 28. 143(7):1919-30

   Insert

   capping protein beta 2 subunit (Capzb Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. T5-pAc5.1 EGFP-EcoRV

   Plasmid

   #206481

   Purpose

   Schneider cell imaging

   Depositor

   Anne Ephrussi

   Article

   Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

   Type

   Empty backbone

   Use

   Tags

   EGFP

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDUAL SRBI (GFP)

   Plasmid

   #86979

   Purpose

   Lentiviral expression construct encoding SR-B1 and GFP from separate promoters

   Depositor

   Joe Grove

   Insert

   SCARB1 (SCARB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV/hPGK

   Available Since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CRBN_ΔHBD

   Plasmid

   #232779

   Purpose

   Expresses CRBN_ΔHBD sequence in E. coli cells with cleavable MBP-His tag and deleted internal DDB1 binding domain

   Depositor

   Ivan Dikic

   Article

   Bailey et al Cell Chem Biol. 2024 Nov 20:S2451-9456(2

   Insert

   Cereblon (CRBN Human)
   

   Use

   Tags

   MBP-His6-TEV

   Expression

   Bacterial

   Mutation

   CRBN (residues 47 to 193 and 249 to 436) with GNG…

   Promoter

   T7

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. sgRNA(MS2) cloning backbone

   Plasmid

   #61424

   Purpose

   sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCG_IFITM1

   Plasmid

   #179955

   Purpose

   Expression vector for human IFITM1.

   Depositor

   Konstantin Sparrer, Frank Kirchhoff

   Article

   Prelli Bozzo et al Nat Commun. 2021 Jul 28;12(1):4584.

   Insert

   IFITM1 (IFITM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   P13A

   Promoter

   Available Since

   Feb. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEGFP-C1-MKLP1

   Plasmid

   #70145

   Purpose

   mammalian expression of MKLP1 with an eGFP fusion

   Depositor

   Masanori Mishima

   Article

   Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

   Insert

   MKLP1 (KIF23 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pWZL Blast DN E-cadherin

   Plasmid

   #18800

   Depositor

   Bob Weinberg

   Article

   Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

   Insert

   Dom neg E-cadherin (Cdh1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   truncated form of E-cadherin (dominant negative) …

   Promoter

   Available Since

   July 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-ccdB-Nanoluc

    Plasmid

    #87067

    Purpose

    Mammalian Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Use

    Tags

    Nanoluc luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pOG44_FLP_recombinase (pEHA1338)

    Plasmid

    #209087

    Purpose

    FLP recombinase for FRT-mediated integration

    Depositor

    Vikram Khurana

    Article

    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

    Insert

    FLP recombinase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-Flag mTOR wt

    Plasmid

    #26603

    Purpose

    Mammalian expression of human flag-tagged mTor

    Depositor

    Jie Chen

    Article

    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

    Insert

    mTOR (MTOR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pInducer10-mir-RUP-PheS

    Plasmid

    #44011

    Purpose

    Lentiviral shRNA vector (pGIPz based) for inducible knockdown

    Depositor

    Stephen Elledge

    Article

    Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

    Insert

    PheS Gly294
    

    Use

    Lentiviral

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    Promoter

    TRE2

    Available Since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pInducer20 ALPK1 Y254C

    Plasmid

    #231587

    Purpose

    ALPK1 gene mutated in position Y254C (Pathogenic mutation in ROSAH syndrome) under control of a doxycycline-inducible promoter

    Depositor

    Thomas Henry

    Article

    Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

    Insert

    ALPK1 (ALPK1 Human)
    

    Use

    Lentiviral

    Tags

    3X flag

    Expression

    Mammalian

    Mutation

    changed tyrosine 254 to cysteine

    Promoter

    doxycycline

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFP-C2-NLRP3

    Plasmid

    #73955

    Purpose

    Express EGFP-tagged human NLRP3 in mammalian cells

    Depositor

    Christian Stehlik

    Article

    Khare et al Immunity. 2012 Mar 23;36(3):464-76. doi:

    Insert

    NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV iE

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PER2:Luc Plasmid

    Plasmid

    #206875

    Purpose

    Per2 promoter luciferase reporter

    Depositor

    Michelle Farkas

    Article

    Lin et al Integr Cancer Ther. 2019 Jan-Dec;18:1534

    Insert

    Period 2 (Per2 Mouse)
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. psfaMs-Tn5

    Plasmid

    #183637

    Purpose

    Expresses anti-mouse nanobody fused with Tn5

    Depositor

    Goncalo Castelo-Branco

    Article

    Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

    Insert

    anti mouse secondary nanobody fused to Tn5
    

    Use

    Tags

    anti-mouse nanobody

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. RFP-LC3B

    Plasmid

    #200943

    Purpose

    RFP fused with LC3B for reporting LC3B lipidation

    Depositor

    Nir Hacohen

    Article

    Liu et al Science. 2023 Aug 4;381(6657):508-514. d

    Insert

    LC3B (MAP1LC3B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKM461

    Plasmid

    #108320

    Purpose

    For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase, and contains SacB

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Inserts

    Che9C RecT
    Bxb1 Integrase
    Tet Repressor
    SacRB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p3E ubb polyA

    Plasmid

    #195977

    Purpose

    Gateway p3E vector

    Depositor

    Christian Mosimann, Alexa Burger

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    ubb:polyA (ubb Zebrafish)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge