We narrowed to 9,122 results for: Pol

1. pCI-neo-μ3A-(HA)3

   Plasmid

   #198179

   Purpose

   Expression of HA-tagged AP-3 μ3A in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Mattera et al PLoS Pathog. 2014 May 15;10(5):e1004107.

   Insert

   AP-3 μ3A
   

   Tags

   triple HA tag

   Expression

   Mammalian

   Mutation

   silent substitution in codon 225

   Promoter

   CMV

   Available Since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBetaActin-FRB-dCer3-CAAX

   Plasmid

   #191326

   Purpose

   Expresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   FRB-dCer-CAAX
   

   Expression

   Mammalian

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBetaActin-TUBB2a-mEos3.2

   Plasmid

   #191320

   Purpose

   Expresses fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   TUBB2a-mEos3.2 (Tubb2a Mouse)
   

   Expression

   Mammalian

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBetaActin-TUBB2a-Dronpa

   Plasmid

   #191321

   Purpose

   Expresses fuorescently labeled beta tubulin to allow photoactivation of microtubule patches with very little UV light

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   TUBB2a-Dronpa (Tubb2a Mouse)
   

   Expression

   Mammalian

   Available Since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMpGE_En03-sgRNA-Target1 (MpRTN1)

   Plasmid

   #186291

   Purpose

   Gateway entry vector for sgRNA (target 1: MpRTN1). Transient expression of sgRNA (Target 1: MpRTN1) for MpRTN1 in plant cells

   Depositor

   Yutaka Kodama

   Article

   Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

   Insert

   sgRNA-Target1
   

   Use

   CRISPR

   Available Since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYTK001_E2At(2nd)

   Plasmid

   #183085

   Purpose

   2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly

   Depositor

   Zhen Wang

   Article

   Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

   Insert

   E2A
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYTK001_O2At(2nd)

   Plasmid

   #183086

   Purpose

   2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly

   Depositor

   Zhen Wang

   Article

   Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

   Insert

   O2A
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYTK001_P2At(2nd)

   Plasmid

   #183087

   Purpose

   2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly

   Depositor

   Zhen Wang

   Article

   Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

   Insert

   P2A
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMpGE010-sgRNA-Target1 (MpRTN1)

   Plasmid

   #186295

   Purpose

   Binary vector for CRISPR/Cas9 (target 1: MpRTN1) in plants (for Agrobacterium-mediated genetic transformation)

   Depositor

   Yutaka Kodama

   Article

   Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

   Insert

   sgRNA-Target1
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-11-LECT2-V40

    Plasmid

    #183031

    Purpose

    Expression of LECT2 V40 variant protein from T7 promoter

    Depositor

    Gareth Morgan

    Article

    Belonogov et al bioRxiv 2022.03.01.482540

    Insert

    LECT2 (LECT2 Human)
    

    Expression

    Bacterial

    Mutation

    V40 variant

    Promoter

    T7

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZS181

    Plasmid

    #177637

    Purpose

    In vitro transcription of Patiria miniata Dishevelled

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

    Insert

    Dishevelled
    

    Use

    In vitro transcription

    Tags

    HaloTag

    Available Since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZS212

    Plasmid

    #177639

    Purpose

    In vitro transcription of Patiria miniata Lamp1

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

    Insert

    Lamp1
    

    Use

    In vitro transcription

    Tags

    mCherry

    Available Since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZS217

    Plasmid

    #177640

    Purpose

    In vitro transcription of Patiria miniata Dishevelled

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

    Insert

    Dishevelled
    

    Use

    In vitro transcription

    Tags

    GFP

    Available Since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pZS221

    Plasmid

    #177643

    Purpose

    In vitro transcription of Patiria miniata Dishevelled

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

    Insert

    Dishevelled
    

    Use

    In vitro transcription

    Tags

    GFP

    Mutation

    K57A, K58A

    Available Since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZS222

    Plasmid

    #177644

    Purpose

    In vitro transcription of Patiria miniata Lamp1

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

    Insert

    Lamp1
    

    Use

    In vitro transcription

    Tags

    3xGFP

    Available Since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. L1-Ck2-smR-chlo

    Plasmid

    #163939

    Purpose

    L1 in position 2, for spectinomycin in chloroplast transformation

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    aadA gene for spectinomycin resistance
    

    Expression

    Bacterial

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pCk1chlo

    Plasmid

    #163942

    Purpose

    Acceptor plasmid for odd level position 1

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    lacZ
    

    Expression

    Bacterial

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pCk2chlo

    Plasmid

    #163943

    Purpose

    Acceptor plasmid for odd level position 2

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    lacZ
    

    Expression

    Bacterial

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCk3chlo

    Plasmid

    #163944

    Purpose

    Acceptor plasmid for odd level position 3

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    lacZ
    

    Expression

    Bacterial

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pCk4chlo

    Plasmid

    #163945

    Purpose

    Acceptor plasmid for odd level position 4

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    lacZ
    

    Expression

    Bacterial

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge