We narrowed to 8,304 results for: construct

1. GA-NES

   Plasmid

   #61018

   Purpose

   ddGFP A-NES

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddGFP A
   

   Use

   Tags

   HindIII site and NES sequence LALKLAGLDIGS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Rabbit GFP Cav 2.2 pMT2

   Plasmid

   #58737

   Purpose

   expression of GFP tagged rabbit Cav2.2 in mammalian cells

   Depositor

   Annette Dolphin

   Article

   Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

   Insert

   Cav2.2 (CACNA1B Rabbit)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1-TRE-U2AF2-TurboID-HA-rtTA

   Plasmid

   #226997

   Purpose

   Integrative plasmid to express U2AF2-TurboID in mammalian cells

   Depositor

   Bruno Dallagiovanna

   Article

   Gomes-Junior et al PLoS One. 2025 May 30;20(5):e0324779. do

   Insert

   U2 small nuclear RNA auxiliary factor 2 (U2AF2 Human)
   

   Use

   Tags

   TurboID-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. RA-NES

   Plasmid

   #61019

   Purpose

   ddRFP A-NES

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddRFP A
   

   Use

   Tags

   HindIII site and NES sequence MNLVDLQKKLEELELDEQQ

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRedCmOcr

   Plasmid

   #176226

   Purpose

   A helper plasmid for expression of the artificial T7ocr-gam-bet-exo operon. The plasmid carries the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage.

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

   Insert

   Lambda Red-T7ocr operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PrhaB and PL

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. MTK2_007

   Plasmid

   #123702

   Purpose

   Encodes human pEF1alpha as a Type 2 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   pEF1a
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. MTK4_002

   Plasmid

   #123811

   Purpose

   Encodes SV40 polyadenylation signal as a Type 4 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   SV40 pA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. MTK4_003

   Plasmid

   #123812

   Purpose

   Encodes rabbit beta-globin polyadenylation signal as a Type 4 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   rglb pA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cav2.1 HA pCAGGS

   Plasmid

   #206076

   Purpose

   expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II

   Depositor

   Annette Dolphin

   Article

   Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

   Insert

   cacna1a (Cacna1a Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/B-actin

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-EF1alpha-EPO-218-PDGFR

    Plasmid

    #139057

    Purpose

    A lentivirus vector to integrate a surface membrane anchored human EPO gene

    Depositor

    Tao Liu

    Article

    Liu et al Cell Chem Biol. 2017 Jun 22;24(6):767-77

    Insert

    erythropoietin(EPO) (EPO Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2 Notch1 ΔEMV-6MT

    Plasmid

    #41737

    Depositor

    Raphael Kopan, Jeffrey Nye

    Article

    Kopan et al Proc Natl Acad Sci U S A. 1996 Feb 20;93

    Insert

    Notch-1 Extracellular-domain-deleted construct (Notch1 Mouse)
    

    Use

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    Contains murine Notch-1 protein fragment lacking …

    Promoter

    Available since

    Jan. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV-GEX-GECO1

    Plasmid

    #32443

    Purpose

    Mammalian expression of green excitation ratiometric genetically encoded Ca2+ indicator for optical imaging

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    GEX-GECO1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pFEndoNA

    Plasmid

    #174724

    Purpose

    Expresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.

    Depositor

    Jukka Finne

    Article

    Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

    Insert

    GFP-endoNA fusion protein
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-NORAD

    Plasmid

    #196086

    Purpose

    Inducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting lncRNA NORAD.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting lncRNA NORAD
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKMV-VioB

    Plasmid

    #65348

    Purpose

    systhesis of vioB gene in the violacein pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    vioB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKMV-VioD

    Plasmid

    #65350

    Purpose

    systhesis of vioD gene in the violacein pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    vioD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-PB2-3x-FLAG-B/BRISBANE/60/2008

    Plasmid

    #187916

    Purpose

    Expresses C terminal 3xFLAG tagged PB2 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    polymerase basic protein 2
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBEG R2-dsRed-L3 #BGV203

    Plasmid

    #48973

    Purpose

    Supplies the selection marker (dsRed) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Insert

    dsRed
    

    Use

    Gateway cloning vector

    Tags

    IRES (strong)

    Expression

    Mutation

    Promoter

    none

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pFEndoNA2

    Plasmid

    #174667

    Purpose

    Expresses GFP-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.

    Depositor

    Jukka Finne

    Article

    Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

    Insert

    GFP-endoNA2 fusion protein
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBEG R2-iBlast-L3 #BGV204

    Plasmid

    #48982

    Purpose

    Supplies the selection marker (blasticidin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Insert

    blasticidin-S deaminase
    

    Use

    Gateway cloning vector

    Tags

    IRES (weak)

    Expression

    Mutation

    Promoter

    na

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only