We narrowed to 4,953 results for: NOG;

1. SnapTag-Binder

   Plasmid

   #179142

   Purpose

   Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).

   Depositor

   Klaus Hahn

   Article

   Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

   Insert

   SspB
   

   Use

   Tags

   SNAP-tag

   Expression

   Mammalian

   Mutation

   Y11K/A15E, residue numbering from pdb: 1ou9

   Promoter

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TRE-UBC-hCD46-T2A-GFP

   Plasmid

   #205457

   Purpose

   lentiviral plasmid for expression of human CD46

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   hCD46 (CD46 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TRE-UBC-Qa1 SCT-T2A-GFP

   Plasmid

   #205453

   Purpose

   lentiviral plasmid for expression of mouse Qa1 SCT

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   Qa1 SCT (H2-T23 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDropIn-T2A-split-intein-Gal4[1-20]-N-int-BlastR

   Plasmid

   #199197

   Purpose

   Insert to create split-intein Gal4[N-int] knock-in vectors, with blasticidin resistance, via Drop-In cloning, released via BsmBI digest

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   T2A-split-intein-Gal4[1-20]-N-int-BlastR
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Flag-mCherry-Binder

   Plasmid

   #179139

   Purpose

   Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).

   Depositor

   Klaus Hahn

   Article

   Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

   Insert

   SspB
   

   Use

   Tags

   FLAG and mCherry

   Expression

   Mammalian

   Mutation

   Y11K/A15E, residue numbering from pdb: 1ou9

   Promoter

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDropIn-T2A-split-intein-Gal4[21-881]-C-int

   Plasmid

   #199196

   Purpose

   Insert to create split-intein Gal4[C-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   T2A-split-intein-Gal4[21-881]-C-int, 3xP3-dsRed
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTRIPZ shIFNAR

   Plasmid

   #127699

   Purpose

   Doxycyclin inducible shRNA knockdown of mouse IFNAR gene

   Depositor

   Sandra Demaria

   Article

   Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

   Insert

   shIFNAR1 Mmu (Ifnar1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAT105

   Plasmid

   #180510

   Purpose

   Plasmid expressing mammalian codon optimized engineered DpbCasX-R3, sgRNAv2 scaffold, and restriction sites to clone in new spacers

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Inserts

   CasX sgRNAv2
   DpbCasX with R3 loop
   

   Use

   CRISPR

   Tags

   2x FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pQLink-GST-10xCTD

   Plasmid

   #138472

   Purpose

   Expresses GST-tagged 10xCTD heptapeptide repeat in bacteria

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

   Insert

   Rpb1 (POLR2A Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   10x CTD repeat

   Promoter

   T5

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUAST-attB-3xHA-V5-RpL10Ab

    Plasmid

    #125223

    Purpose

    3xHA-V5 tagged RpL10Ab for expression from UAS promoter to TRAP translatome from specific cell types defined by GAL4 expression

    Depositor

    Herman Dierick

    Article

    Thomas et al PLoS One. 2012;7(7):e40276. doi: 10.1371

    Insert

    RpL10Ab (RpL10Ab Fly)
    

    Use

    Tags

    3x HA, V5

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAFW-Ago2

    Plasmid

    #50554

    Purpose

    Expression of FLAG tagged Ago2 in S2 cell

    Depositor

    Yukihide Tomari

    Article

    Iwasaki et al Mol Cell. 2010 Jul 30;39(2):292-9. doi:

    Insert

    Argonaute2 (AGO2 Fly)
    

    Use

    Tags

    FLAG

    Expression

    Insect

    Mutation

    Promoter

    Actin5C

    Available since

    Feb. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDEST-HemmarG

    Plasmid

    #31221

    Depositor

    Yuh-Nung Jan

    Article

    Han et al Proc Natl Acad Sci U S A. 2011 May 23. (

    Insert

    CD4-tdGFP (CD4 Fly, Aequorea victoria, Human)
    

    Use

    Tags

    CD4 and EGFP/GFP

    Expression

    Insect

    Mutation

    Fusion of signal peptide of Drosophila Akh gene, …

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAc-y1sgRNA-Cas9

    Plasmid

    #49331

    Purpose

    Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

    Depositor

    Ji-Long Liu

    Article

    Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

    Inserts

    Cas9
    dU6-sgRNA
    y1 sgRNA (y Fly)
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Insect

    Mutation

    Human codon optimised

    Promoter

    Actin-5c and Drosophila U6

    Available since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. KHBD00476

    Plasmid

    #39585

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG5838 (Dref Fly)
    

    Use

    Gateway donr vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

15. UAS-dsarm(E)

    Plasmid

    #187870

    Purpose

    Gal4/UAS expression of dSarm(isoE)

    Depositor

    Lukas Neukomm

    Article

    Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

    Insert

    dSarm isoform E (Sarm Fly)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. yPet_cp229-Binder

    Plasmid

    #179137

    Purpose

    YPet circularly permuted at a.a. 229; Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9).

    Depositor

    Klaus Hahn

    Article

    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

    Insert

    SspB
    

    Use

    Tags

    yPet_cp229

    Expression

    Mammalian

    Mutation

    Y11K/A15E, residue numbering from pdb: 1ou9

    Promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 13XLexAop2 IVS ChrimsonR mCherry_tr

    Plasmid

    #111548

    Purpose

    Optogenetics in Drosophila: LexAop2-induced expression of ChrimsonR mCherry

    Depositor

    Vivek Jayaraman

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR_mCherry_trafficked
    

    Use

    Tags

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCas9-beta-mmr

    Plasmid

    #169241

    Purpose

    Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Inserts

    cas9
    beta
    mutL-E36K
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Changed glutamic acid 36 to lysine

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAc5.1B-lambdaN-HA-DmeIF4G-V5His6

    Plasmid

    #79259

    Purpose

    Expresses lambdaN-HA-tagged and C-terminal V5-His6-tagged DmeIF4G in S2 cells

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al EMBO J. 2013 Apr 3;32(7):1052-65. doi: 1

    Insert

    DmeIF4G (eIF4G1 Fly)
    

    Use

    Schneider 2 cell expression

    Tags

    V5-His6 and lambdaN-HA

    Expression

    Mutation

    Promoter

    Ac5 promoter

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. YW249_Pzipt17-loxP-H2BGFP-miniMOS

    Plasmid

    #196063

    Purpose

    a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of SL2::H2B::GFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and GFP cassettes.

    Depositor

    Wenxing Yang

    Article

    Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

    Inserts

    H2B::GFP
    NeoR
    Minimal Mos1
    zipt17p::zipt17::zipt17 3UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge