We narrowed to 9,202 results for: sacs

1. p416TEF-NYFP

   Plasmid

   #99553

   Purpose

   Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].

   Depositor

   Liming Li

   Article

   Du et al Mol Cell Biol. 2010 Oct;30(19):4644-55.

   Insert

   Swi1 N-rich region (AA1-327) (SWI1 Budding Yeast)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Aug. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. POM43-mCitrine

   Plasmid

   #69855

   Purpose

   N-terminal tagging with mCitrine using LEU2 as a selection marker in S. cerevisiae.

   Depositor

   Liza Pon

   Article

   Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

   Insert

   mCitrine minus start and stop codon
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEPOR2KN0040

   Plasmid

   #117607

   Purpose

   Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_NbPDS(pEPOR1CB0058)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   [35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNB774

   Plasmid

   #60114

   Purpose

   Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   GrLuc
   

   Use

   Unspecified

   Tags

   ClpXP degron and Nuclear localization signal (NLS…

   Expression

   Mutation

   Promoter

   Available since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX302-NIPP1-V5 puro

   Plasmid

   #87775

   Purpose

   Expresses V5 tagged human NIPP1

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   NIPP1 (PPP1R8 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-kanMX6-P41nmt1-mEGFP

   Plasmid

   #105140

   Purpose

   Yeast gene targeting

   Depositor

   Jian-Qiu Wu

   Article

   Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110.

   Insert

   kanMX6-P41nmt1-mEGFP
   

   Use

   Tags

   41nmt1-mEGFP

   Expression

   Bacterial and Yeast

   Mutation

   A206K

   Promoter

   41nmt1

   Available since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET151-Vta1(280-330)

   Plasmid

   #87738

   Purpose

   Bacterial expression of the VSL domain of S. cerevisiae Vta1

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)
   

   Use

   Tags

   6xHis-V5-TEV

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-279

   Promoter

   T7

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDA125

   Plasmid

   #74249

   Purpose

   Intermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pSTL1 (UbiY 2xNLSs SZ1).

   Depositor

   Serge Pelet

   Article

   Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

   Insert

   Inducible peptide of the dPSTR
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   pSTL1

   Available since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pILGFPQ3

   Plasmid

   #83558

   Purpose

   Used to evaluate the expression output of GAL2 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

   Insert

   GAL2 promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL2

   Available since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAG416GPD-Cerulean-CHC1

    Plasmid

    #18850

    Depositor

    Susan Lindquist

    Article

    Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

    Insert

    CHC1 (CHC1 Budding Yeast)
    

    Use

    Tags

    Cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pPS833

    Plasmid

    #1358

    Depositor

    Pamela Silver

    Article

    Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

    Insert

    GSP1 (GSP1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    G21V, stabilizes the GTP-bound form

    Promoter

    Available since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

12. pRPB1-CTD14

    Plasmid

    #112822

    Purpose

    pSMF2 with 14 CTD repeats

    Depositor

    Stephen Fuchs

    Article

    Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    CTD repeats have identical sequence, deleted repe…

    Promoter

    Native promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRS406 ATG1

    Plasmid

    #166846

    Purpose

    Expresses Atg1 with ATG1 3'UTR in yeasts.

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

    Insert

    ATG1 (ATG1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ATG1 Promoter

    Available since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Gal4pBS4-Pleum

    Plasmid

    #60329

    Purpose

    Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 11, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pDM3193

    Plasmid

    #113390

    Purpose

    Alpha factor inducible Ty3 with his3AI

    Depositor

    Suzanne Sandmeyer

    Article

    Bilanchone et al PLoS Genet. 2015 Sep 30;11(9):e1005528.

    Insert

    Ty3-his3AI
    

    Use

    Tags

    his3AI

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSP571

    Plasmid

    #139498

    Purpose

    CRISPR-Cas9 plasmid to generate double strand break in STL1 locus in S. cerevisiae. Expresses both Cas9 and STL1 sgRNA

    Depositor

    Serge Pelet

    Article

    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

    Insert

    pGPD Cas9 / sgRNA (STL162)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    pGPD

    Available since

    June 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. S3_004_026_MV1

    Plasmid

    #134416

    Purpose

    pYTKMV1-pTDH3_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1

    Depositor

    Mattheos Koffas

    Article

    Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

    Inserts

    mCherry
    FdeR-N_2xNLS
    

    Use

    Tags

    none

    Expression

    Yeast

    Mutation

    none

    Promoter

    pGMP1 and pTDH3

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 907K

    Plasmid

    #160236

    Purpose

    FlightN-PipR-VP16-Sv40-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    pristinamycin repressor
    Flightin promoter
    

    Use

    Tags

    VP16

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. A102-GAP-BoCCS-GRM

    Plasmid

    #123286

    Purpose

    Expression of Glo3 ΔAmpH (1-459) in E.Coli

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    ADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPROEX Hsp104 (773-908)

    Plasmid

    #1316

    Depositor

    Susan Lindquist

    Article

    Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

    Insert

    Hsp104 (773-908) (HSP104 Budding Yeast)
    

    Use

    Tags

    6X His + TEV cleavage

    Expression

    Bacterial

    Mutation

    deletion of aa1-773

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pPROEX Hsp104 (361-548)

    Plasmid

    #1317

    Depositor

    Susan Lindquist

    Article

    Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

    Insert

    Hsp104 (361-548) (HSP104 Budding Yeast)
    

    Use

    Tags

    6X His + TEV cleavage

    Expression

    Bacterial

    Mutation

    aa361-548 of Hsp104

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AB.pCCL.sin.cPPT.GFP.miR-210-3p.sensor.PGK.dNGFR.WPRE

    Plasmid

    #85920

    Purpose

    Sensor Plasmid

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-210-3p Sensor (MIR210 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pES1002

    Plasmid

    #21789

    Depositor

    Richard Yu

    Article

    Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

    Insert

    Fus3-as2 (FUS3 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    contains 5' 1kb upstream UTR/promoter Q93…

    Promoter

    Available since

    Oct. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AB.pCCL.sin.cPPT.U6.miR-325-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46610

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-325 Decoy
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

25. pTH651-RLuc/minFLuc

    Plasmid

    #29697

    Depositor

    Tobias von der Haar

    Article

    Chu et al Nucleic Acids Res. 2011 Aug;39(15):6705-

    Insert

    Firefly Luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    All codons of the original FLuc gene were exchang…

    Promoter

    Available since

    Aug. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGADT7 Hsh155 Y292C

    Plasmid

    #87291

    Purpose

    Encodes Y2H Gal4 activation domain for Hsh155 Y292C

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

    Insert

    HSH155 (HSH155 Budding Yeast)
    

    Use

    Tags

    GAL4 AD and HA

    Expression

    Bacterial and Yeast

    Mutation

    Y292C

    Promoter

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEPOR2KN0079

    Plasmid

    #117646

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_ENTH/ANT {AT1G14910}(pEPOR1CB0100)and sgRNA_ENTH/ANT {AT2G01600}(pEPOR1CB0111)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. JBEI-6822

    Plasmid

    #84985

    Purpose

    For expression of pBbA5c-MevTaco-MKco-PMKco(aka 2A) under lacUV5 promoter for isopentenol production

    Depositor

    Taek Soon Lee

    Article

    George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

    Insert

    MevTaco-MKco-PMKco
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LacUV5

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJLI-SUP35 (delta)2-5

    Plasmid

    #1138

    Depositor

    Susan Lindquist

    Article

    Liu et al Nature 1999 Aug 5;400(6744):573-6.

    Insert

    sup35 delta 2-5 mutant, 5' and 3' flanking regions (SUP35 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    last four repeats of sup35 N domain deleted, but …

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AB.pCCL.sin.cPPT.U6.miR-125b-3p-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46582

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-125b-3p Decoy
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only