We narrowed to 4,895 results for: phy

1. p3xFLAG-CMV-Drosha (P426)

   Plasmid

   #171537

   Purpose

   Encodes mutant human Drosha protein

   Depositor

   Yan Zeng

   Article

   Zhang et al Biochim Biophys Acta Gene Regul Mech. 20

   Insert

   DROSHA (DROSHA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   a Drosha mutant starting from P426

   Promoter

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3xFLAG-CMV-Drosha (E419)

   Plasmid

   #171534

   Purpose

   Encodes mutant human Drosha protein

   Depositor

   Yan Zeng

   Article

   Zhang et al Biochim Biophys Acta Gene Regul Mech. 20

   Insert

   DROSHA (DROSHA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   a Drosha mutant stopping at E419

   Promoter

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCK-Drosha-FLAG(delta6)

   Plasmid

   #171532

   Purpose

   Encodes mutant human Drosha protein

   Depositor

   Yan Zeng

   Article

   Zhang et al Biochim Biophys Acta Gene Regul Mech. 20

   Insert

   DROSHA (DROSHA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   small deletions of Drosha_1271A to 1276G_

   Promoter

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-4T-1-Dicer-dsRBD (+6)

   Plasmid

   #171544

   Purpose

   Encodes a Dicer fragment extending from from R1841 to the C-terminus

   Depositor

   Yan Zeng

   Article

   Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

   Insert

   DICER1 (DICER1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   a six amino acid_TRLTEG_insertion in the L1 region

   Promoter

   Available since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-EF1a-HaloSFPQY527A

   Plasmid

   #166949

   Purpose

   Lentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from the EF1a promoter

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   SFPQ-Y527A (SFPQ Human)
   

   Use

   Lentiviral

   Tags

   Halo

   Expression

   Mutation

   SFPQ-Y527A

   Promoter

   eF1a

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. UbC-Halo-SFPQY527A

   Plasmid

   #166947

   Purpose

   Lentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from the UbC promoter

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   SFPQ-Y527A (SFPQ Human)
   

   Use

   Lentiviral

   Tags

   Halo

   Expression

   Mutation

   SFPQ-Y527A

   Promoter

   UbC

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. CBSH3- shRNA2

   Plasmid

   #160210

   Purpose

   Knock Down MTLSH3- Collybistin isoforms

   Depositor

   Angel de Blas

   Article

   George et al J Neurochem. 2021 May;157(4):1032-1051.

   Insert

   ARHGEF9 (Arhgef9 Human, Rat)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   mouse U6 promoter

   Available since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. CBSH3- shRNA3

   Plasmid

   #160212

   Purpose

   Knock Down MYDSH3- Collybistin isoforms

   Depositor

   Angel de Blas

   Article

   George et al J Neurochem. 2021 May;157(4):1032-1051.

   Insert

   ARHGEF9 (Arhgef9 Human, Rat)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   mouse U6 promoter

   Available since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. CBSH3- shRNA1

   Plasmid

   #160208

   Purpose

   Knock Down MQWSH3- Collybistin isoforms

   Depositor

   Angel de Blas

   Article

   George et al J Neurochem. 2021 May;157(4):1032-1051.

   Insert

   ARHGEF9 (Arhgef9 Human, Rat)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   mouse U6 promoter

   Available since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP(1-233)-mApple(2-236)-HRas(2-189)

    Plasmid

    #133855

    Purpose

    Mammalian expression of HRas N-terminally tagged with EGFP and mApple

    Depositor

    Dave Piston

    Article

    Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

    Insert

    HRas (HRAS Human)
    

    Use

    Tags

    EGFP and mApple

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJC20cdc8.27

    Plasmid

    #99362

    Purpose

    Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.27.

    Depositor

    Dan Mulvihill

    Article

    Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

    Insert

    cdc8 (cdc8 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Glutamic acid 129 to Lysine

    Promoter

    T7

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKI_ΩBBMVP16&SRDXWUSm1

    Plasmid

    #220349

    Purpose

    Expresses BBM-VP16 and SRDX-WUSm1 in plant cells

    Depositor

    Tomoko Igawa

    Article

    Sato et al Front Plant Sci. 2024 Apr 3;15:1308417.

    Inserts

    BABY BOOM (BBM Mustard Weed)
    WUSCHEL (WUS Mustard Weed)
    

    Use

    Tags

    Superman Repression Domain X (SRDX) and VP16 tran…

    Expression

    Plant

    Mutation

    two amino acid substitution in WUS-box: L255A L25…

    Promoter

    CaMV 35S and RPS5A

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastbac1-Flag-FANCD2

    Plasmid

    #134904

    Purpose

    codon optimized expression and purification of human FANCD2 in insect cells using baculovirus

    Depositor

    Andrew Deans

    Article

    Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

    Insert

    FANCD2 (FANCD2 Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    fully synthetic, codon optimized for insect expre…

    Promoter

    polyhedron

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Cre-IRES-PuroR

    Plasmid

    #30205

    Purpose

    Lentiviral coexpression of Cre and puromycin from the human EF1a promoter

    Depositor

    Darrell Kotton

    Article

    Somers et al Stem Cells. 2010 Oct . 28(10):1728-40.

    Insert

    Cre recombinase (cre Enterobacteria phage P1)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    April 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. LentiGuide Blast

    Plasmid

    #199622

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and blasticidin resistance from EF-1a promoter.

    Depositor

    Danwei Huangfu

    Article

    Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. p37-2iDMD-LR

    Plasmid

    #88892

    Purpose

    37°C full-length DMD luc-red plasmid. Co-expresses human dystrophin, firefly luciferase and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites and can be grown at 37°C.

    Depositor

    Michele Calos

    Article

    Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/

    Inserts

    Dystrophin (DMD Human)
    luciferase
    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3 AIF 1-90 mCherry FRB

    Plasmid

    #67530

    Purpose

    For expression of mCherry fused FRB to the mitochondrial innermembrane

    Depositor

    Stephen Tait

    Article

    Ichim et al Mol Cell. 2015 Mar 5;57(5):860-72. doi:

    Inserts

    AIF a.a. 1 - 90
    mCherry
    FRB
    

    Use

    Tags

    AIF 1 - 90, FRB, and mCherry

    Expression

    Mammalian

    Mutation

    T->L

    Promoter

    CMV

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pStreptagIIx2 Alfatag 3C InsR EABR

    Plasmid

    #234996

    Purpose

    For production of Extracellular Vesicles (EVs), with the Insulin Receptor Strep-tag II labeled on their surface

    Depositor

    Daniel Leahy, Monica Gonzalez-Magaldi

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    InsR-EABR (INSR Human)
    

    Use

    Tags

    2xStreptag-II, Alfatag, HRV 3C site

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRRL_SRSF2_P95H_mCherry

    Plasmid

    #84023

    Purpose

    expression of SRSF2 mutant in mammalian cells

    Depositor

    Robert Bradley

    Article

    Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

    Insert

    SRSF2 (SRSF2 Human)
    

    Use

    Lentiviral

    Tags

    2TA-mCherry and Flag

    Expression

    Mammalian

    Mutation

    P95H

    Promoter

    PGK

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV GFAP jGCaMP7b

    Plasmid

    #171118

    Purpose

    Astrocyte AAV-mediated expression of bright ultrasensitive protein calcium sensor under the GFAP promoter

    Depositor

    Thomas Oertner

    Article

    Lohr et al Front Cell Neurosci. 2021 Jun 11;15:6901

    Insert

    jGCaMP7b
    

    Use

    AAV

    Tags

    6 His Tag, T7 Tag, and Xpress Tag

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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