We narrowed to 6,266 results for: wus

1. 3HA-ERHBD-Hoxb8

   Plasmid

   #212932

   Purpose

   Expresses an estrogen-regulated form of Hoxb8 to generate conditionally immortalized hematopoietic progenitor cell lines

   Depositor

   Hans Haecker

   Article

   Redecke et al Nat Methods. 2013 Aug;10(8):795-803. doi

   Insert

   3HA-ERHBD-Hoxb8 (ESR1, Hoxb8 Mouse, Human)
   

   Use

   Retroviral

   Tags

   Triple HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV LTR

   Available Since

   Jan. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-EF1a-fDIO-Cre

   Plasmid

   #121675

   Purpose

   Expresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)

   Depositor

   Alexander Nectow, Esteban Engel

   Article

   Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

   Has Service

   AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

   Insert

   NLS-CRE-HA
   

   Use

   AAV

   Tags

   3xHA and SV40-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHR-SNAP-CD86iso1

   Plasmid

   #223601

   Purpose

   Lentiviral expression of human CD86 isoform 1 with SNAP tag in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   CD86 isotype1 (CD86 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   SNAP

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMIR-21 Reporter

   Plasmid

   #71870

   Purpose

   Mammalian expression vector for the analysis of miR-21 activity

   Depositor

   Mien-Chie Hung

   Article

   Shen et al Nature. 2013 May 16;497(7449):383-7. doi

   Insert

   Reverse complementary sequence of miR-21
   

   Use

   Tags

   firefly luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTMB352_ZFcharm_Kv1_split-inteins

   Plasmid

   #220848

   Purpose

   Lentiviral vector expressing ZFcharm Kv2 fused to ZF3 and MmZFP_81187 via split Npu inteins; perfect match ZF3 binding site located upstream of pEFS

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   ZFcharm Kv2
   

   Use

   Lentiviral

   Tags

   P2A-mCherry

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   EFS

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA

   Plasmid

   #61593

   Purpose

   A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

   Depositor

   Feng Zhang

   Article

   Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

   Inserts

   hSaCas9
   Chimeric guide for SaCas9
   

   Use

   AAV and CRISPR

   Tags

   HA, NLS, and OLLAS tag

   Expression

   Mammalian

   Mutation

   K175R and K736R (deUb mutations)

   Promoter

   Available Since

   Feb. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-CAG-SV40NLSf-GFP-3xmiR122-WPRE-HGHpA

   Plasmid

   #183775

   Purpose

   AAV genome with a CAG driven eGFP reporter with mR122 target sequence repeats to reduce transgene expression in hepatocytes.

   Depositor

   Ben Deverman

   Article

   Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

   Insert

   NLS-GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   CAG

   Available Since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MSCV-Tox-IRES-eGFP

   Plasmid

   #165427

   Purpose

   Expresses TOX in mammalian cells

   Depositor

   Anjana Rao, Patrick Hogan

   Article

   Seo et al Proc Natl Acad Sci U S A. 2019 Jun 18;11

   Insert

   TOX (Tox Mouse)
   

   Use

   Tags

   3xflag 5' of Tox

   Expression

   Mammalian

   Mutation

   Promoter

   5' LTR

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MSCV-Tox2-IRES-eGFP

   Plasmid

   #165428

   Purpose

   Expresses TOX2 in mammalian cells

   Depositor

   Anjana Rao, Patrick Hogan

   Article

   Seo et al Proc Natl Acad Sci U S A. 2019 Jun 18;11

   Insert

   TOX2 (Tox2 Mouse)
   

   Use

   Tags

   3xflag 5' of Tox2

   Expression

   Mammalian

   Mutation

   Promoter

   5' LTR

   Available Since

   March 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-K-GECO1

    Plasmid

    #105865

    Purpose

    Mammalian cell expression plasmid for red fluorescent Ca2+ indicator K-GECO1

    Depositor

    Robert Campbell

    Article

    Shen et al BMC Biol. 2018 Jan 16;16(1):9. doi: 10.1

    Insert

    K-GECO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Flag-ZDHHC21

    Plasmid

    #231669

    Purpose

    Expresses human ZDHHC21protein

    Depositor

    Chunhong Ma

    Article

    Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

    Insert

    ZDHHC21 (ZDHHC21 Human)
    

    Use

    Tags

    3×Flag Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTMB301_ZFcharm_Kv1_Prnp_SPM

    Plasmid

    #220840

    Purpose

    AAV genome expressing ZFcharm Kv1 targeting Prnp; single perfect match self-silencing binding site

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    ZFcharm Kv1
    

    Use

    AAV

    Tags

    HAtag

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EFS

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FLAG-PRMT6

    Plasmid

    #164697

    Purpose

    Expression of PRMT6 with an N-terminal FLAG tag

    Depositor

    Cheryl Arrowsmith

    Article

    Eram et al ACS Chem Biol. 2016 Mar 18;11(3):772-781

    Insert

    PRMT6 (PRMT6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHR-Spy-CD86

    Plasmid

    #223598

    Purpose

    Lentiviral expression of human CD86 with SpyTag003 in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD86 (CD86 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    Spytag003

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pENTR1A-TAZ S89A

    Plasmid

    #179911

    Purpose

    Entry vector of TAZ S89A for gateway coloning.

    Depositor

    Xin Chen

    Article

    Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(

    Insert

    WW Domain Containing Transcription Regulator 1 (WWTR1 Human)
    

    Use

    Entry vector

    Tags

    3X Flag-tag

    Expression

    Mutation

    Serine 89 mutated to Alanine

    Promoter

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHR-SNAP-PDL1

    Plasmid

    #223602

    Purpose

    Lentiviral expression of human PD-L1 with SNAP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    PD-L1 (CD274 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    SNAP

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-His6-ERK2-MEK1_R4F_coexpression

    Plasmid

    #39212

    Depositor

    Melanie Cobb

    Article

    Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

    Inserts

    Mek1 R4F (MAP2K1 Human)
    Erk2 (Mapk1 Rat)
    

    Use

    Tags

    His6 and RBS

    Expression

    Bacterial

    Mutation

    MEK1(R4F) = S218E, S222D and deletion of AA32-51

    Promoter

    T7 and T7 via RBS

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-CAG-Cre-3xmiR122-WPRE-HGHpA

    Plasmid

    #183776

    Purpose

    AAV genome with a CAG driven Cre recombinase with mR122 target sequence repeats to reduce transgene expression in hepatocytes.

    Depositor

    Ben Deverman

    Article

    Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

    Insert

    Cre
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    CAG

    Available Since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRK5-HA-Ubiquitin-K11

    Plasmid

    #121152

    Purpose

    Mammalian expression of HA tagged ubiquitin with K11 only, other lysines mutated to arginines

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All Ks apart from K11 mutated to Rs

    Promoter

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti6/V5-DEST-NCAPH

    Plasmid

    #31214

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    NCAPH (NCAPH Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge