Addgene

We narrowed to 9,565 results for: Ada;

Showing: 3941 - 3960 of 9565 results

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pBabe hygro NFS1 CD

Plasmid

#102978

Purpose

Expression of NFS1 C381A mutant

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

NFS1 C381A (NFS1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

C381A catalytic site mutant, Silent mutations to …

Promoter

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-FLAG-GFP-Ubl4A-C(90-157)

Plasmid

#86923

Purpose

To express Ubl4A (90-157 residues) tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Use

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Residues from 1-89 are deleted

Promoter

CMV

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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spa-GCaMP6m

Plasmid

#67557

Purpose

photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6m

Use

Tags

HIS-tag

Expression

Mammalian

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Promoter

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-PPARgamma2 2KR

Plasmid

#78772

Purpose

To overexpress PPARgamma2 2KR in Mammalian Cells

Depositor

Jaewhan Song

Article

Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

Insert

PPAR_2 (Ppargc1a Mouse)

Use

Tags

Expression

Mammalian

Mutation

K184A, K185A

Promoter

CMV

Available since

June 10, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-RIPK3 V460P

Plasmid

#78814

Purpose

To overexpress RIPK3 V460P in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

V460P

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-STAG2-Q735Stop

Plasmid

#31977

Depositor

Todd Waldman

Article

Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

Insert

human STAG2 cDNA (STAG2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

changed Glutamine 735 to Stop codon

Promoter

Available since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
Luc-Nanog-3'UTRmut

Plasmid

#63894

Purpose

Luciferase reporter of mouse Nanog 3'UTR with the miR-34 target site mutated

Depositor

Lin He

Article

Choi et al Nat Cell Biol. 2011 Oct 23;13(11):1353-6

Insert

Nanog homeobox (Nanog Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2015

Availability

Academic Institutions and Nonprofits only
sspa-GCaMP6m

Plasmid

#67560

Purpose

short photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

sspa-GCaMP6m

Use

Tags

Expression

Mammalian

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Promoter

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA 5/FR/TO-Ubl4A K48R

Plasmid

#61811

Purpose

express C-terminal Ubl4A K48R, for making the stable cell line

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

UBL4A (UBL4A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

K48R

Promoter

CMV

Available since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only
EC.SurA.pTYB1.a21-281&390-428.L239R

Plasmid

#15959

Depositor

David McKay

Article

Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

Insert

Escherichia coli surA delta PPIase domain 2

Use

Tags

intein

Expression

Bacterial

Mutation

residues 282-389 deleted; L239R mutation

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
3187 pSFFV-neo Bad cDNA ORF

Plasmid

#8756

Depositor

Stanley Korsmeyer

Article

Zha et al Cell 1996 Nov 15;87(4):619-28.

Insert

BAD (Bad Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only
NHA-Ago3m

Plasmid

#21532

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago3 (AGO3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

missing aa757-823, the C-termianl 66aa of PIWI do…

Promoter

Available since

Sept. 4, 2009

Availability

Academic Institutions and Nonprofits only
TAL3016

Plasmid

#41190

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-adam15-Left (LOC795199 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
TAL3017

Plasmid

#41191

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-adam15-Right (LOC795199 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
mycUb-25aa

Plasmid

#20256

Depositor

Nico Dantuma

Article

Verhoef et al FASEB J. 2009 Jan . 23(1):123-33.

Insert

Ubiquitin-25 amino acid extension (RBX1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Ubiquitin fused to N-terminus of GFP. Glycine 76 …

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pEGFP-STAG2-D299A

Plasmid

#31975

Depositor

Todd Waldman

Article

Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

Insert

human STAG2 cDNA (STAG2 Human)

Use

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

changed Aspartic acid 299 to Alanine

Promoter

Available since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
pBABE FLAG HA p100

Plasmid

#236468

Purpose

stable expression of p100 using virus infection

Depositor

Michele Pagano

Article

Busino et al Nat Cell Biol. 2012 Mar 4;14(4):375-85.

Insert

p100 (NFKB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 2xFLAG-2xSTREP TFDP2

Plasmid

#236445

Purpose

transient overexpression of TFDP2 in mammalian cells

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

TFDP2 (TFDP2 Human)

Use

Tags

FLAG-FLAG-STREP-STREP

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pUC18 HA PTPL1

Plasmid

#237335

Purpose

expression in bacterial cells for protein purification

Depositor

Michele Pagano

Article

Kuchay et al Nat Cell Biol. 2013 May;15(5):472-80. do

Insert

PTPL1 (PTPN13 Human)

Use

Tags

HA

Expression

Bacterial

Mutation

Promoter

Available since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA FLAG p85beta

Plasmid

#237336

Purpose

transient overexpression in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nat Cell Biol. 2013 May;15(5):472-80. do

Insert

p85beta (PIK3R2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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