Addgene

We narrowed to 23,156 results for: Nov

Showing: 9851 - 9900 of 23156 results

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B2-1

Plasmid

#111468

Purpose

Ile-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Ile-tRNA synthetase

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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B5-1

Plasmid

#111480

Purpose

Leu-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Leu-tRNA synthetase

Use

Tags

6xHis

Expression

Bacterial

Mutation

I10K

Promoter

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEB1-mVenus JBC

Plasmid

#103987

Purpose

Plasmid encoding mVenus JBC

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mVenus JBC

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F46L, F64L, …

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-SCFP1

Plasmid

#103969

Purpose

Plasmid encoding SCFP1

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

SCFP1

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, S65T, …

Promoter

proC

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pVITRO1-mCherry-CAAX

Plasmid

#186347

Purpose

Plasma membrane-targeted mCherry expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

mCherry-CAAX

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pVITRO1-mCherry-NLS

Plasmid

#186346

Purpose

Nucleus-targeted mCherry expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

mCherry-3xNLS

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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BiDi-[mp-mCherry2]mp-mEGFP

Plasmid

#182871

Purpose

Bi-directional expression vector of monomers with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al Biophys J. 2021 Dec 21;120(24):5478-5490

Insert

myr-palm-mCherry2, myr-palm-mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pVITRO1-iRFP702-NLS

Plasmid

#184454

Purpose

Nucleus-targeted iRFP702 expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

iRFP702-3xNLS

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pVITRO1-iRFP702-CAAX

Plasmid

#184455

Purpose

Plasma membrane-targeted iRFP702 expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

iRFP702-CAAX

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pKM498

Plasmid

#134215

Purpose

Baculovirus expression of CENP-T and CENP-W

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

his-CENPT + CENPW

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac211-msGFP2-STE13

Plasmid

#177106

Purpose

Expresses msGFP2-Ste13 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

STE13 (STE13 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pVITRO1-iRFP702-NES

Plasmid

#184457

Purpose

Cytosolic iRFP702 expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

iRFP702-NES

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-18

Plasmid

#79933

Purpose

PH-4GS-I3-01-myc-Late1

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-18

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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Mouse NCKX6 (pcDNA3.1+)

Plasmid

#75215

Purpose

Mammalian expression of SLC8B1

Depositor

Jonathan Lytton

Article

Cai et al J Biol Chem. 2004 Feb 13;279(7):5867-76.

Insert

SLC8B1 (Slc8b1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pAWH-Rluc-let-7-A114-N40-HhR

Plasmid

#50555

Purpose

Expression of luciferase reporter mRNA in S2 cell

Depositor

Yukihide Tomari

Article

Fukaya et al Mol Cell. 2012 Dec 28;48(6):825-36. doi:

Insert

Luciferase-let7-A114-N40-HhR

Use

Luciferase

Tags

Expression

Insect

Mutation

Promoter

Actin5C

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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YIplac211-STV1-iGFP

Plasmid

#177111

Purpose

Expresses Stv1-iGFP in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

STV1 (STV1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a(+)-HpDsbG

Plasmid

#39329

Depositor

SeWon Suh

Article

Yoon et al FEBS Lett. 2011 Dec 15;585(24):3862-7. E

Insert

oxidoreductase

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
pKM610

Plasmid

#134218

Purpose

Baculovirus expression of CENP-R

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-R untagged

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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prLCA-Citrine

Plasmid

#40271

Depositor

Martin Offterdinger

Article

Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

Insert

Clta (Clta Rat)

Use

Tags

mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only

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p1177-X1-8

Plasmid

#38150

Depositor

David Waugh

Article

Austin et al Protein Expr Purif. 2011 May;77(1):53-61

Insert

Carboxypeptidase A

Use

Tags

HHHHHHRR

Expression

Insect

Mutation

Promoter

polyhedron

Available since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only

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a4 ecto pcDNA3.1 hygro(-)

Plasmid

#113406

Purpose

expression of human alpha 4 ectodomain with purification tags and C-terminal clasp

Depositor

Timothy Springer

Article

Li et al J Cell Biol. 2018 Jan 2;217(1):397-412.

Insert

human alpha 4 ectodomain

Use

Tags

Strep II tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAtLCYbSK

Plasmid

#53588

Purpose

Produces an active Arabidopsis thaliana lycopene beta-cyclase enzyme in E. coli. Also known as pAt9SK.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Eukaryot Cell. 2007 Mar;6(3):533-45. Epu

Insert

lcyB (LYC Mustard Weed)

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pUG-UBC2

Plasmid

#104667

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBC2 (UBC2 Mustard Weed)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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MS2-ecDHFR

Plasmid

#65859

Purpose

Expresses RNA-binding protein MS2 with eDHFR fusion tag

Depositor

Aaron Hoskins

Article

Carrocci et al Analyst. 2014 Jan 7;139(1):44-7. doi: 10

Inserts

MS2
E. coli Dihydrofolate Reductase (folA )

Use

Tags

HA and NLS

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pAWH-Rluc-let-7-Poly(A)

Plasmid

#50556

Purpose

Expression of luciferase reporter mRNA in S2 cell

Depositor

Yukihide Tomari

Article

Fukaya et al Mol Cell. 2012 Dec 28;48(6):825-36. doi:

Insert

Luciferase-let7-Poly(A)

Use

Luciferase

Tags

Expression

Insect

Mutation

Promoter

Actin5C

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pTlpAreverse-Wasabi

Plasmid

#86114

Purpose

mWasabi reporter gene driven by TlpA promoter in reverse orientation

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA
mWasabi

Use

Tags

6xHis

Expression

Bacterial

Mutation

D341A

Promoter

TlpA (in reverse orientation)

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pHES553 [prGal1-RAS2(KAN) pnh604]

Plasmid

#103846

Purpose

Yeast Expression Plasmid

Depositor

Hana El-Samad

Article

Stewart-Ornstein et al Mol Biol Cell. 2017 Jan 1;28(1):221-227.

Insert

pGal1-Ras2(KAN)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
EF2451_pET21a(+)

Plasmid

#13840

Depositor

SeWon Suh

Article

Kang et al Acta Crystallograph Sect F Struct Biol C

Insert

PPAT

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

June 29, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
B3-4

Plasmid

#111474

Purpose

translational release factor 2

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational release factor 2

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcpmAmetrine

Plasmid

#42170

Depositor

Robert Campbell

Article

Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

Insert

cpmAmetrine173

Use

Tags

6His

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only
pUG-UBC27

Plasmid

#104692

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBC27 (UBC27 Mustard Weed)

Use

Synthetic Biology

Tags

Expression

Mutation

BpiI/BsaI sites removed by point-mutation

Promoter

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKM568

Plasmid

#134217

Purpose

Baculovirus expression of CENP-C

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

GST-CENP-C

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pARiBo1

Plasmid

#98892

Purpose

Expresses ARiBo-fused RNA in vitro with T7 RNA polymerase

Depositor

Pascale Legault

Article

Di Tomasso et al Nucleic Acids Res. 2011 Feb;39(3):e18. d

Insert

ARiBo1

Use

In vitro transcription vector

Tags

Expression

Mutation

Promoter

T7 promoter

Available since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-EPN-03

Plasmid

#79918

Purpose

Myr2-12GS-I3-01-myc-Late1

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-03

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pTlpAsp-Wasabi

Plasmid

#86113

Purpose

mWasabi reporter gene driven by TlpA promoter with scrambled Pribnow box

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Insert

mWasabi

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

pTlpA (scrambled Pribnow box)

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-EPN-07

Plasmid

#79922

Purpose

Palm1-12GS-I3-01-myc-Late1

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-07

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pCMV-EPN-20

Plasmid

#79935

Purpose

Late2-4GS-I3-01-myc-PH

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-20

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pCMV-EPN-01

Plasmid

#79916

Purpose

Myr1-12GS-I3-01-myc-Late1

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pRGM10

Plasmid

#48790

Purpose

I-SceI DSB repair reporter cassette

Depositor

Michael Glickman

Article

Gupta et al Mol Microbiol. 2011 Jan;79(2):316-30. do

Inserts

lacZ(I-SceI)
lacZ-deltaN

Use

Dsb repair reporter

Tags

Expression

Mutation

Promoter

Available since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
Tg-M71(161 mHD+ O/E,176)-LacZpA

Plasmid

#15606

Depositor

Peter Mombaerts

Article

Vassalli et al Mol Cell Neurosci. 2011 Feb;46(2):381-96

Insert

Tg-M71(161 mHD+ O/E,176)-LacZpA

Use

High copy number

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pTcINDEX-GW

Plasmid

#234713

Purpose

Used to express any CDS in T. cruzi in a tetracycline regulatable manner. Modified from Taylor et al. (2006) for easy cloning using Gateway recombination cassette.

Depositor

Esteban Serra

Article

Alonso et al Mem Inst Oswaldo Cruz. 2014 Dec;109(8):1

Type

Empty backbone

Use

Trypanosomatid expression

Tags

Expression

Mutation

Promoter

Available since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGlab592

Plasmid

#236554

Purpose

240 basepair upstream sequence of KSHV ORF68 driving HaloTag expression

Depositor

Britt Glaunsinger

Article

Morgens et al Mol Syst Biol. 2025 Jan;21(1):30-58. doi

Insert

KSHV ORF68 upstream 240bp

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTYB1-T4gp46

Plasmid

#228211

Purpose

Expression in BL21(DE3) of T4 SbcC-like subunit of palindrome specific endonuclease

Depositor

Stephen Benkovic

Article

Herdendorf et al J Biol Chem. 2011 Jan 28;286(4):2382-92.

Insert

46

Use

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
lvl1-S2S3 [backbone]

Plasmid

#229195

Purpose

lvl 1 backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
lvl1-S4S5 [backbone]

Plasmid

#229197

Purpose

lvl 1 backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
lvl1-S3S4 [backbone]

Plasmid

#229196

Purpose

lvl 1 backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
lvl2-[landingPad-backbone]

Plasmid

#229210

Purpose

lvl 1 backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
lvl2-[piggyBac-backbone]

Plasmid

#229209

Purpose

lvl 1 backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
lvl1-S1S2 [rev-backbone]

Plasmid

#229199

Purpose

lvl 1 backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
lvl1-S1S2 [backbone]

Plasmid

#229194

Purpose

lvl 1 backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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