We narrowed to 4,010 results for: SPL
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Plasmid#215545PurposeDonor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-outDepositorInsertB2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived puromycin-GFP selection cassette (B2M Human)
UseCRISPRMutationThe second base of the first intron of B2M (From …Available SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-NEDD1-gTBD
Plasmid#196878PurposeNeuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.DepositorInsertmOrange2-N-gTBD
UseCre/LoxExpressionMammalianPromoterQuimeric CAGAvailable SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-inv[Talpha1-Dre-pA]-lox-mOrange2-Cdk5rap2-CTD
Plasmid#196881PurposeNeuron-specific expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.DepositorInsertmOrange2-Cdk5rap2-CTD
UseCre/Lox; Dre/roxExpressionMammalianPromoterQuimeric CAGAvailable SinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-Syk-tSH2-FX
Plasmid#111272Purposeexpress murine Syk tandem SH2 domains (Ser 8 to Gln 264), with interdomain A (Phe 119 to His 162) substituted by a flexible 20-amino-acid linker (GGS)3GS(GGS)3, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk tandem SH2 domains (Ser 8 to Gln 264), with interdomain A (Phe 119 to His 162) substituted by a flexible 20aa linker (Syk Mouse)
ExpressionBacterialMutationinterdomain A (Phe 119 to His 162) substituted by…PromoterT7 promoterAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLHCX-XBP1 mNeonGreen NLS
Plasmid#115971PurposeER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen (retroviral vector backbone)DepositorInsertX-box binding protein 1 (XBP1 Synthetic, Human)
UseRetroviralTagsHA tag, c-myc NLS, and mNeonGreenExpressionMammalianPromoterCMVAvailable SinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-XBP1 mNeonGreen NLS
Plasmid#115968PurposeER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreenDepositorInsertX-box binding protein 1 (XBP1 Synthetic, Human)
UseLentiviralTagsHA tag, c-myc NLS, and mNeonGreenExpressionMammalianPromoterCMVAvailable SinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-Lyn11-mEGFP-cpHalo∆-(GGS)9-FKBP-P2A-Hpep3-(GGS)3-FRB-mScarlett
Plasmid#205703PurposeCMV driven co-expression of split HaloTag FKBP- and FRB-fusions (Lyn11-EGFP-FKBP-cpHalo∆ and Hpep1-FRB-mScarlett) in mammalian cell linesDepositorInsertLyn11-EGFP-FKBP-cpHalo∆-P2A-Hpep1-FRB-mScarlett
ExpressionMammalianPromoterCMVAvailable SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-KIF1B beta-FL
Plasmid#79108PurposeMammalian expression of GFP-KIF1B beta-FLDepositorInsertGFP-KIF1B beta full length (KIF1B Human)
TagsGFPExpressionMammalianMutationexon 25 missing (natural splice varient)PromoterCMVAvailable SinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
PE7_AAVS1_knockin_HDR_donor
Plasmid#223001PurposeHDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_armDepositorInsertPE7
UseHdr donorTagsSV40 bpNLS and c-Myc NLSExpressionMammalianPromoterEF1αAvailable SinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28.His.3C.86b.Tau2N4R
Plasmid#226399PurposeExpresses his-tagged, 3C-cleavable, 86b (split luciferase fragment)-tagged WT 2N4R tau for recombinant protein production in bacteriaDepositorAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE (Supernova)
Plasmid#69138PurposeFor the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivoDepositorInsertCAG-loxP-stop-loxP-RFP-ires-tTA-WPRE
TagsnoneExpressionMammalianAvailable SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-CCreV
Plasmid#140132Purposecan be used to generate AAV virus that will express fusion protein of split Cre (C-terminal) and fungal photoreceptor Vivid (VVD) monomerDepositorInsertCCreV
UseAAVPromoterEF1aAvailable SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-NCreV
Plasmid#140131Purposecan be used to generate AAV virus that will express fusion protein of split Cre (N-terminal) and fungal photoreceptor Vivid (VVD) monomerDepositorInsertNCreV
UseAAVPromoterEF1aAvailable SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
RFP-KIF1B beta-FL
Plasmid#79111PurposeMammalian expression of RFP-KIF1B beta-FLDepositorInsertRFP-KIF1B beta full length (KIF1B Human)
TagsRFPExpressionMammalianMutationexon 25 missing (natural splice varient)PromoterCMVAvailable SinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
lenti U6-sgRNA-acRNA SYN-dCas9-P2A-EGFP
Plasmid#159086PurposeExpresses dCas9-P2A-EGFP driven by human SYN promoter and empty CRISPR Display sgRNA/accessory RNA from U6 promoter.DepositorInsertempty crRNA-acRNA backbone, dCas9-P2A-EGFP
UseCRISPR and LentiviralTagsEGFP and FLAGExpressionMammalianPromoterhSYN, U6Available SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIRIGF-IKZF1-V2
Plasmid#69045Purposeexpression of IKZF1 fused to firefly luciferase with co-expression of Renilla luciferase, both under IRES control.DepositorInsertIKZF1 (IKZF1 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationsplice variant 2PromoterIRESAvailable SinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-CKAP4 S3E S17E S19E-WPRE-UbC-Emerald
Plasmid#225947PurposeLentiviral vector plasmid expressing human CKAP4 mutations S3E S17E S19E under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralExpressionMammalianMutationS3E S17E S19EPromoterSFFV and UbCAvailable SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-CKAP4 S3A S17A S19A-WPRE-UbC-Emerald
Plasmid#225948PurposeLentiviral vector plasmid expressing human CKAP4 mutations S3A S17A S19A under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralExpressionMammalianMutationS3A S17A S19APromoterSFFV and UbCAvailable SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
plenti-CAG-IKZF1-V1-Q146H-FLAG-IRES-GFP
Plasmid#69048Purposelentiviral expression of IKZF1 variant 1 with Q146H mutation and FLAG tagDepositorInsertIKZF1 (IKZF1 Human)
UseLentiviralTagsFLAG and IRES-GFPExpressionMammalianMutationsplice variant 1, Q146HPromoterCAGAvailable SinceOct. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irTN6kwh
Plasmid#44724DepositorInsertspCMV-D2i promoter
htetR::NLS
UseSynthetic Biology; Expression regulator/reporter;…TagsRabbit β-globin intron II and WPREExpressionMammalianMutationInitiator motif (Inr) displaced relative to pCMV-…PromoterpCMV-D2iAvailable SinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only