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We narrowed to 15,864 results for: cas9

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Showing: 3941 - 3960 of 15864 results

  1. pCMV-T7-SpCas9(RRTIMR)-P2A-EGFP (AHK149)

    Plasmid
    #223071
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with RRTIMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(RRTIMR)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(RRTIMR)=D1135R, S1136R, G1218T, E1219I, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223071-plasmid-map-sequence-id-447687

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  2. pCMV-T7-SpCas9(IRAVQL)-P2A-EGFP (RTW3820)

    Plasmid
    #223073
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with IRAVQL amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(IRAVQL)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(IRAVQL)=D1135I, S1136R, G1218A, E1219V, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223073-plasmid-map-sequence-id-447648

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  3. pCMV-T7-SpCas9(SRSCMV)-P2A-EGFP (RTW3732)

    Plasmid
    #223074
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with SRSCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(SRSCMV)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(SRSCMV)=D1135S, S1136R, G1218S, E1219C, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223074-plasmid-map-sequence-id-447690

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  4. pCMV-T7-SpCas9(MWNVML)-P2A-EGFP (AHK156)

    Plasmid
    #223076
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MWNVML amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(MWNVML)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(MWNVML)=D1135M, S1136W, G1218N, E1219V, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223076-plasmid-map-sequence-id-447684

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  5. pCMV-T7-SpCas9(MKKCMN)-P2A-EGFP (AHK129)

    Plasmid
    #223077
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MKKCMN amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(MKKCMN)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(MKKCMN)=D1135M, S1136K, G1218K, E1219C, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223077-plasmid-map-sequence-id-447665

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  6. pCMV-T7-SpCas9(KKKCMN)-P2A-EGFP (AHK131)

    Plasmid
    #223078
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KKKCMN amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(KKKCMN)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(KKKCMN)=D1135K, S1136K, G1218K, E1219C, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223078-plasmid-map-sequence-id-447649

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  7. pCMV-T7-SpCas9(ARGIMR)-P2A-EGFP (AHK147)

    Plasmid
    #223069
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with ARGIMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(ARGIMR)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(ARGIMR)=D1135A, S1136R, G1218G, E1219I, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223069-plasmid-map-sequence-id-447645

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  8. pCMV-T7-SpCas9(ARTWGR)-P2A-EGFP (RAS2995)

    Plasmid
    #223070
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with ARTWGR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(ARTWGR)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(ARTWGR)=D1135A, S1136R, G1218T, E1219W, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223070-plasmid-map-sequence-id-447646

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  11. dCas9-GCN5 FL

    Plasmid
    #179552
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Publication
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 (KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    179552-plasmid-map-sequence-id-354751

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  14. pLenti_dCas9-2xAM_hIRF-1

    Plasmid
    #92221
    Purpose
    Lentiviral plasmid expressing dCas9-2xAM and gRNA for human IRF-1 promoter
    Depositor
    Hodaka Fujii
    Publication
    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do
    Inserts
    Sp-dCas9-2xAM tag
    gRNA targeting human IRF-1 promoter
    Use
    CRISPR and Lentiviral
    Tags
    2xAM tag
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    CBh and U6
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    92221-plasmid-map-sequence-id-181086

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  19. SpyCas9 PE2*-3HA

    Plasmid
    #169854
    Purpose
    Mammalian Expression, SpyCas9 PE2* prime editor with 3HA-tag
    Depositor
    Scot Wolfe
    Publication
    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-H840A-star-M-MLV-3HA
    Tags
    3XHA tag, bpSV40 NLS and SV40 NLS, and cmyc-NLS a…
    Expression
    Mammalian
    Promoter
    cmv
    Available since
    June 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    169854-plasmid-map-sequence-id-332900

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Showing: 3941 - 3960 of 15864 results