We narrowed to 9,083 results for: mel

1. pHDR_NANOS3-T2A-mVenus-PuroTK

   Plasmid

   #222903

   Purpose

   Homology directed repair template for knocking in mVenus reporter to NANOS3.

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   mVenus
   

   Use

   CRISPR

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1a-double floxed-Aldh1a1-HA-WPRE-HGHpA

   Plasmid

   #184633

   Purpose

   Expresses Aldh1a1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

   Depositor

   Nicolas Tritsch

   Article

   Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

   Insert

   Aldh1a1 (Aldh1a1 Mouse)
   

   Use

   AAV

   Promoter

   EF1a

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. dmBACCS2VL-IRES-dOrai

   Plasmid

   #67629

   Purpose

   Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOrai

   Depositor

   Takao Nakata

   Article

   Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

   Insert

   dmBACCS2VL-IRES-dOrai
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. 1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]

   Plasmid

   #149426

   Purpose

   The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

   Depositor

   Omar Akbari

   Article

   Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

   Inserts

   U6.3-gRNAs[Sxl, bTub]
   Hsp70Bb-Cas9-T2A-eGFP-p10
   

   Use

   CRISPR

   Tags

   eGFP

   Expression

   Insect

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLL7.0-iRFP670-micro

   Plasmid

   #135954

   Purpose

   Expresses an iLID micro (SspB R73Q)-iRFP670 fusion in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   iLID micro (SspB R73Q)
   

   Use

   Lentiviral

   Tags

   iRFP670

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSB1C3_YPet-PCP (y-plasmid)

   Plasmid

   #127637

   Purpose

   Encodes a fluorecent protein with an RNA binding peptide: YPet-PCP.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme RiboJ and RBS BBa_B0034.

   Depositor

   Friedrich Simmel

   Article

   Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.

   Insert

   YPet
   

   Use

   Synthetic Biology

   Tags

   RNA binding peptide: PP7 coat protein (PCP)

   Expression

   Bacterial

   Promoter

   pJ21306

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. dmBACCS2NS-IRES-dOrai

   Plasmid

   #67628

   Purpose

   Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOrai

   Depositor

   Takao Nakata

   Article

   Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

   Insert

   dmBACCS2NS-IRES-dOrai
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJ207 Delta-cpc-KanR

   Plasmid

   #51468

   Purpose

   Deletes the cpc operon in Synechocystis and confers kanamycin resistance

   Depositor

   Anastasios Melis

   Article

   Kirst et al Biochim Biophys Acta. 2014 Oct;1837(10):

   Insert

   Replacing the cpc operon with kanamycin resistance
   

   Use

   Synthetic Biology

   Promoter

   Synechocystis endogenous cpc-operon promoter

   Available Since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRP_8xgRNA-FLuc

   Plasmid

   #135937

   Purpose

   Firefly luciferase reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   minCMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYO1124

    Plasmid

    #235725

    Purpose

    Expression of human UVRAG (1-464) fused to human ATG14L BATS (413-492) in mammalian cells

    Depositor

    Roger Williams

    Article

    Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

    Insert

    UVRAG(1-464) fused to ATG14L BATS domain (413-492)
    

    Expression

    Mammalian

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJB180

    Plasmid

    #235737

    Purpose

    Expression of mCherry- human RAB1A Q70L in mammalian cells

    Depositor

    Roger Williams

    Article

    Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

    Insert

    RAB1A (RAB1A Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Q70L

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYO1296

    Plasmid

    #235710

    Purpose

    Expression of mCherry-human RAB5A Q79L in mammalian cells

    Depositor

    Roger Williams

    Article

    Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

    Insert

    RAB5 (RAB5A Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Q79L, aa 2-215

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYO1262

    Plasmid

    #235727

    Purpose

    Protein expression of His6-RAB1A Q70L 1-203 in bacteria

    Depositor

    Roger Williams

    Article

    Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

    Insert

    RAB1A (RAB1A Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Q70L, aa 1-203

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHDR_NPM2-T2A-mGreenLantern-PuroTK

    Plasmid

    #222910

    Purpose

    Homology directed repair template for knocking in mGreenLantern reporter to NPM2.

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    mGreenLantern
    

    Use

    CRISPR

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHDR_FIGLA-T2A-mGreenLantern-PuroTK

    Plasmid

    #222905

    Purpose

    Homology directed repair template for knocking in mGreenLantern reporter to FIGLA.

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    mGreenLantern
    

    Use

    CRISPR

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHDR2_TFAP2C-T2A-mGreenLantern-Hygro

    Plasmid

    #222915

    Purpose

    Homology directed repair template for knocking in mGreenLantern reporter to TFAP2C.

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    mGreenLantern
    

    Use

    CRISPR

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPB-cT3G-cERP2-ZGLP1

    Plasmid

    #222926

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of ZGLP1

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    ZGLP1 (ZGLP1 Human)
    

    Expression

    Mammalian

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPB-cT3G-cERH-SOHLH1

    Plasmid

    #222922

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of SOHLH1

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    SOHLH1 (SOHLH1 Human)
    

    Expression

    Mammalian

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRISPRa_all-in-one_Bip gRNA1

    Plasmid

    #183696

    Purpose

    Expresses gRNA targeting chinise hamster Bip (Hspa5) with CRISPRa components and mCherry, blasticidin selective

    Depositor

    Edward Avezov

    Article

    Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

    Insert

    gRNA targeting chinese hamster Bip (Hspa5)
    

    Use

    CRISPR

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-RAPH1Δ536-587

    Plasmid

    #194865

    Purpose

    Express eGFP-RAPH1 lacking amino acids 536-587 in mammalian cells. This constructs does not localise to filopodia anymore.

    Depositor

    Guillaume Jacquemet

    Article

    Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

    Insert

    RAPH1 (RAPH1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    RAPH1 with amino acids 536-587 deleted

    Promoter

    CMV

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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