We narrowed to 26,374 results for: sta

1. pTP434

   Plasmid

   #104167

   Purpose

   Bacterial expression plasmid of anti-GFP nanobody with 3x Cysteines for maleimide labeling

   Depositor

   Dirk Görlich

   Article

   Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

   Insert

   Anti-GFP nanobody Enhancer PDB 3K1K (3x Cysteine)
   

   Use

   Tags

   14x Histidine tag and NEDD8 from Brachypodium dis…

   Expression

   Bacterial

   Mutation

   3x Cysteines located at bps 493-495, 520-522, and…

   Promoter

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. K785R Smarca4-V5 IRES-puro

   Plasmid

   #107059

   Purpose

   Expression of dominant-negative K785R Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.

   Depositor

   Jerry Crabtree, Courtney Hodges

   Article

   Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

   Insert

   SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
   

   Use

   Lentiviral

   Tags

   IRES-puroR and V5

   Expression

   Mammalian

   Mutation

   K785R

   Promoter

   CAG

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-eGFP-NLS-RNF168

   Plasmid

   #133977

   Purpose

   mammalian expression vector of eGFP tagged wild type RNF168

   Depositor

   Daniel Durocher

   Article

   Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

   Insert

   RNF168 (RNF168 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   siRNA resistant sequence: GAGGAGTCGTGTTTATTGA

   Promoter

   CMV

   Available since

   Nov. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pLAP-BUBR1

   Plasmid

   #114052

   Purpose

   transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

   Depositor

   Geert Kops

   Article

   Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

   Insert

   BUBR1 (BUB1B Human)
   

   Use

   Tags

   YFP-TEV-S- Human LAP

   Expression

   Mammalian

   Mutation

   C2823A and G2826A (siRNA-resistance)

   Promoter

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pD2sE_SC14

   Plasmid

   #219717

   Purpose

   DV2 stable E dimer

   Depositor

   Brian Kuhlman

   Article

   Kudlacek et al Sci Adv. 2021 Oct 15;7(42):eabg4084. doi

   Insert

   DV2 sE SC14
   

   Use

   Tags

   8xHis and human serum albumin (HSA)

   Expression

   Mammalian

   Mutation

   A259W, T262R, F279W, T280P

   Promoter

   Available since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti CMV Puro DHFR.cHSF1

   Plasmid

   #134739

   Purpose

   Lentiviral expression vector for constitutively active HSF1 fused to DHFR destabilized domain

   Depositor

   Matthew D. Shoulders

   Article

   Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

   Insert

   DHFR.cHSF1 (HSF1 Human)
   

   Use

   Lentiviral

   Tags

   DHFR

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 186-202

   Promoter

   CMV

   Available since

   Aug. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIgK-2.1-FLAG

   Plasmid

   #225829

   Purpose

   Mouse IgK chain specific for chicken gamma globulin (clone 2.1). Used to produce FLAG-tagged monoclonal antibodies for use as tag-specific ELISA standards.

   Depositor

   Gabriel Victora

   Article

   Schiepers et al Nature. 2023 Mar;615(7952):482-489. doi:

   Insert

   Mouse anti-CGG IgK chain, Flag-tagged
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIgK-2.1-StreptagII

   Plasmid

   #225830

   Purpose

   Mouse IgK chain specific for chicken gamma globulin (clone 2.1). Used to produce StreptagII-tagged monoclonal antibodies for use as tag-specific ELISA standards.

   Depositor

   Gabriel Victora

   Article

   Schiepers et al Nature. 2023 Mar;615(7952):482-489. doi:

   Insert

   Mouse anti-CGG IgK chain, Streptag-II-tagged
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. RpH-ILV-FRB

   Plasmid

   #225137

   Purpose

   RpH-ILV probe strongly colocalises with lysosomal markers with FRB

   Depositor

   Massimiliano Stagi

   Article

   Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

   Insert

   TMEM192 (TMEM192 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. px330-GAPDH

    Plasmid

    #136940

    Purpose

    NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human GAPDH; induce CD4+ deletion rearrangement by pairing w/ px330-CD4

    Depositor

    Jeremy Stark

    Article

    Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

    Insert

    sgRNA targeting GAPDH (GAPDH Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. DN2-Gli3 in AINGpCAGGS

    Plasmid

    #121969

    Purpose

    strong Gli3 transcriptional activator

    Depositor

    James Briscoe

    Article

    Stamataki et al Genes Dev. 2005 Mar 1;19(5):626-41. doi:

    Insert

    DN2Gli3 (GLI3 Human)
    

    Use

    Chick electroporation

    Tags

    5xmyc

    Expression

    Mammalian

    Mutation

    N terminus deletion (nt1-1401)

    Promoter

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1_hE2F7mg

    Plasmid

    #73073

    Purpose

    human E2F7 minigene (exons 11,12,13/introns cassette)

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    E2F7 (E2F7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    partial gene, exons 11,12,13 spaced by introns

    Promoter

    CMV

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPBbsr2-Necl-5-ScNeo

    Plasmid

    #170283

    Purpose

    To monitor the status of Necl-5, the plasmid encodes a recombinant Necl-5 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

    Insert

    a recombinant mouse Necl-5 fused to the mScarlet and to the mNeonGreen fluorescent protein (Pvr Synthetic, Mouse)
    

    Use

    Tags

    mScarlet and mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV14_NHP2L1(15.5kD)

    Plasmid

    #73065

    Purpose

    expression clone for human NHP2L1 (15.5kD) with C-terminal 3xFLAG-tag

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    NHP2L1 (15.5kD) (SNU13 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pD2sE_SC10

    Plasmid

    #219716

    Purpose

    DV2 stable E dimer with fusion loop mutation

    Depositor

    Brian Kuhlman

    Article

    Kudlacek et al Sci Adv. 2021 Oct 15;7(42):eabg4084. doi

    Insert

    DV2 sE SC10
    

    Use

    Tags

    8xHis and human serum albumin (HSA)

    Expression

    Mammalian

    Mutation

    G106D, A259W, T262R, F279W, T280P

    Promoter

    Available since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONOR223 R2pH-LAMP1-3xFLAG

    Plasmid

    #157941

    Purpose

    gateway donor vector encoding ratiometric sensor of lysosomal lumenal pH

    Depositor

    Massimiliano Stagi

    Article

    Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

    Insert

    R2pH-LAMP1-3xFLAG (Lamp1 Mouse)
    

    Use

    Gateway system

    Tags

    3XFLAG, mCherry, and pHluorin

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. ePB-PURO-FLAG-METTL3-APPA

    Plasmid

    #160253

    Purpose

    For stable integration of catalytic inactive FLAG-METTL3 in human cells with trasposase

    Depositor

    Alessandro Fatica

    Article

    Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

    Insert

    methyltransferase like 3 (METTL3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changes in aa 395–398, DPPW → APPA

    Promoter

    tight TRE promoter

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSAVED-CHAT_PCaspase_PCi_PC-σ

    Plasmid

    #214040

    Purpose

    Bacterial expression plasmid for SAVED-CHAT, PCaspase, PCi, and PC-σ from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT-Pcaspase-Pci-PC-σ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    lacUV5 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL3-B-161;+80pCalb2

    Plasmid

    #66745

    Purpose

    luciferase reporter for Calb2 promoter (-161to +80, WT)

    Depositor

    Emanuela Felley-Bosco, Rolf Stahel

    Article

    Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

    Insert

    Calb2 promoter (-161bp+80) (CALB2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Calb2

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3-B-264;+80pCalb2

    Plasmid

    #66746

    Purpose

    luciferase reporter for Calb2 promoter (-264 to +80, WT)

    Depositor

    Emanuela Felley-Bosco, Rolf Stahel

    Article

    Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

    Insert

    Calb2 promoter (-264bp+80) (CALB2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Calb2

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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