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pMonAID_dCas9-PmCDA_Hyg_ALS

Plasmid

#91692

Purpose

Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls

Depositor

Akihiko Kondo

Publication

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A and H840A for dead Cas9

Promoter

2x35S

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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G-GECO1-Orai1

Plasmid

#73561

Purpose

Green fluorescent reporter of Orai1-associated calcium influx

Depositor

Michael Cahalan

Publication

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NM_032790.3 (ORAI1 Human, Synthetic)

Tags

G-GECO1

Expression

Mammalian

Promoter

CMV Immediate Early

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLSLGFP

Plasmid

#51494

Purpose

pLSL with GFP followed by the us:NPTII repair template, Rep is not included on this plasmid

Depositor

Daniel Voytas

Publication

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

GFP
us:NPTII repair template

Use

Plant t-dna plasmid

Tags

NLS and flag

Promoter

virion-sense LIR and 2x35S

Available since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LT1_36_QUEEN-2m

Plasmid

#177708

Purpose

Plasmid for genome integration in X2 site expressing QUEEN-2m (ATP biosensor)

Depositor

Lisbeth Olsson

Publication

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

pTEF1-QUEEN2m

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Flp-DOG

Plasmid

#80084

Purpose

Flp recombinase dependent on GFP (Flp-DOG) for expression in mammalian cells

Depositor

Connie Cepko

Publication

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Insert

Flp-DOG

Expression

Mammalian

Promoter

CAG

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pShuttle-FEN1DA

Plasmid

#35028

Depositor

Sheila Stewart

Publication

Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

Insert

FEN1DA (FEN1 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

D181A--NUCLEASE DEAD POINT MUTANT

Promoter

CMV

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-PRKAR1B

Plasmid

#23376

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKAR1B (PRKAR1B Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PRKG2

Plasmid

#23435

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKG2 (PRKG2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-PSDΔ1,2-FRBx3

Plasmid

#225827

Purpose

A component of SYNCit-K (Synapse-targeted chemically induced translocation of Kalirin-7)

Depositor

Haruo Kasai

Publication

Sawada et al Science. 2024 Sep 27;385(6716):1459-1465

Insert

PSDΔ1,2-FRBx3

Use

AAV

Expression

Mammalian

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pFlp-Tc

Plasmid

#222336

Purpose

Expresses Flp and tetracycline to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-TetAR-pBBR1-ELT3

Depositor

Philip Poole

Publication

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

sacB (sacB )
flp (flp )
cI (cI )

Promoter

cl, flp, and sacB

Available since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSH824-ssr

Plasmid

#198027

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Publication

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

T0 terminator - Apr resistance cassette

Expression

Bacterial

Promoter

AprR promoter

Available since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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P4 cosmid

Plasmid

#196335

Purpose

P4 cosmid with CRISPR-Cas9 system

Depositor

Baojun Wang

Publication

Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

Insert

genes from phage P4, CRISPR-Cas9 system

Use

CRISPR

Expression

Bacterial

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRM006

Plasmid

#98922

Purpose

Express Histag-SUMO-FtsZ for purification in E coli

Depositor

Jie Xiao

Publication

Yang et al Science. 2017 Feb 17;355(6326):744-747.

Insert

FtsZ

Tags

Histag-SUMO

Expression

Bacterial

Promoter

T7

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV Puro-CCSP:GFP

Plasmid

#68487

Purpose

Retrovirus expressing GFP driven by CCSP promoter

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Puromycin Resistance
GFP

Use

Retroviral

Expression

Mammalian

Promoter

CCSP and LTR

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pVRb20_992

Plasmid

#49714

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pecf20_992

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCS6-mPSD95a-TS2-HA

Plasmid

#42240

Depositor

Roger Tsien

Publication

Butko et al Nat Neurosci. 2012 Nov;15(12):1742-51. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PSD95

Tags

TS2-HA

Expression

Mammalian

Available since

March 13, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR223-MAP4K2

Plasmid

#23644

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP4K2 (MAP4K2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pLPC MYC hTel2

Plasmid

#22802

Depositor

Titia de Lange

Publication

Takai et al Cell. 2007 Dec 28. 131(7):1248-59.

Insert

Telomere Maintenance 2 (TELO2 Human)

Use

Retroviral

Tags

MYC

Expression

Mammalian

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pAAV-CMV-BDNF-Myc

Plasmid

#124279

Purpose

Express Myc-tagged brain-derived neurotrophic factor (BDNF)

Depositor

Baoji Xu

Publication

Xie et al Cell Metab. 2019 Jan 11. pii: S1550-4131

Insert

BDNF (Bdnf Mouse)

Use

AAV

Tags

Myc

Available since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pPSSUB-101

Plasmid

#26580

Depositor

Ron Weiss

Publication

Basu et al Proc Natl Acad Sci U S A. 2004 Apr 27. 1

Insert

destabilized GFP

Expression

Bacterial

Mutation

S2R, C-terminal extension RPAANDENYA(LVA)

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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