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pBW829_pCAG-pExpr-LUT

Plasmid

#87554

Purpose

Encodes Boolean Look-up Table logic in response to Cre, Flp, PhiC31, Vika, B3, and Bxb1 recombinases

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pVH234-Tier1-PmPGK1-tTA

Plasmid

#169574

Purpose

Tier-1 vector encoding PmPGK1-driven tTA expression (PmPGK1-tTA-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled transactivator

Expression

Mammalian

Promoter

PPGK

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pBP-mTAZ-NFLAG

Plasmid

#31791

Depositor

Michael Yaffe

Article

Hong et al Science. 2005 Aug 12;309(5737):1074-8.

Insert

TAZ (Wwtr1 )

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
cmv 16 E7 del PTLHE

Plasmid

#13688

Depositor

Karl Munger

Article

Gonzalez et al J Virol. 2001 Aug . 75(16):7583-91.

Insert

HPV 16 E7 del PTLHE (E7 Human)

Expression

Mammalian

Mutation

del aa 6-10

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-ABEmax(TadA, TadA*E59A)

Plasmid

#125662

Purpose

pCMV-ABEmax(TadA, TadA*E59A) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A TadA* E59A)

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCGN R-Ras 41A

Plasmid

#14730

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

Insert

R-ras (RRAS Human)

Tags

HA

Expression

Mammalian

Mutation

41A

Available Since

Aug. 29, 2007

Availability

Academic Institutions and Nonprofits only
pMaCTag-27

Plasmid

#120006

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with 1xHA

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

1xHA

Use

Pcr template

Tags

1xHA

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTK101

Plasmid

#59561

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) mKate-G8-L7Ae-PEST 4xFF4

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) mKate-G8-L7Ae-PEST 4xFF4

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pGL2B -688/+64

Plasmid

#24944

Depositor

Stephen Smale

Article

Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

Insert

IL-10 promoter (Il10 Mouse)

Use

Luciferase

Tags

Luciferase

Available Since

Aug. 18, 2010

Availability

Academic Institutions and Nonprofits only
pGL2B -78/+64

Plasmid

#24947

Depositor

Stephen Smale

Article

Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

Insert

IL-10 promoter (Il10 Mouse)

Use

Luciferase

Tags

Luciferase

Available Since

Aug. 18, 2010

Availability

Academic Institutions and Nonprofits only
pST1374-Lb-Cpf1-NLS

Plasmid

#107680

Purpose

Expresses LbCpf1 in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

LbCpf1

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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IFP1.4-C1

Plasmid

#54782

Purpose

Localization: C1 Cloning Vector, Excitation: 684, Emission: 708

Depositor

Michael Davidson, Roger Tsien

Article

Shu et al Science. 2009 May 8;324(5928):804-7. doi

Type

Empty backbone

Tags

IFP1.4

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
MTK3b_004

Plasmid

#123785

Purpose

Encodes mAzamiGreen as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

mAzamiGreen

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-SYN-PIK3CD

Plasmid

#203730

Purpose

AAV vector plasmid expressing human phosphoinositide 3-kinase catalytic subunit delta (PIK3CD) under the human synapsin (SYN) promoter

Depositor

Richard Eva, Bart Nieuwenhuis

Insert

PIK3CD (PIK3CD Human)

Use

AAV

Expression

Mammalian

Promoter

Human synapsin promoter

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTagRFP675-actin-C1

Plasmid

#44277

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

Insert

TagRFP675

Tags

Actin

Expression

Mammalian

Mutation

M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

Promoter

CMV

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pJM410 ZF1x6-C mKate2 in TUPV1

Plasmid

#161526

Purpose

Inducible expression of mKate2 under the ZF1x6-C promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

mKate2

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF1x6-C

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAG149 NeuroD 3'UTR

Plasmid

#12007

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N14 3'UTR (NEUROD1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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IZ1181

Plasmid

#21049

Depositor

Tadahide Izumi

Article

Busso et al Oncogene. 2009 Apr 2. 28(13):1616-25.

Insert

human MDM2 (MDM2 Human)

Tags

no tag

Expression

Mammalian

Mutation

no mutation. This is the wild-type human MDM2.

Available Since

July 9, 2009

Availability

Academic Institutions and Nonprofits only
EB3-eGFP-2xC2-iLID

Plasmid

#114419

Purpose

anchor iLID domain to plasma membrane with slower diffusion

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

iLID domain

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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MTK2_013

Plasmid

#123705

Purpose

Encodes a spacer (for multicistronic) as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pSpacer multicistronic

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-IT10

Plasmid

#66871

Purpose

Inntag 10: a small self-structured epitope aimed at innocuous protein tagging

Depositor

Marti Aldea

Article

Georgieva et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

Insert

Inntag 10

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only
pAGM9121_pDHAS

Plasmid

#160920

Purpose

Dihydroxyacetone synthase (DHAS) promoter module for episomal P. pastoris promoter shuffling

Depositor

Sylvestre Marillonnet

Article

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

Dihydroxyacetone synthase promoter

Use

Synthetic Biology

Mutation

BsaI and BbsI domesticated

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_pFDH1

Plasmid

#160913

Purpose

Formate dehydrogenase (FDH1) promoter module for episomal P. pastoris promoter shuffling

Depositor

Sylvestre Marillonnet

Article

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

formate dehydrogenase promoter

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_pDAS1

Plasmid

#160916

Purpose

Dihydroxyacetone synthase 1 (DAS1) promoter module for episomal P. pastoris promoter shuffling

Depositor

Sylvestre Marillonnet

Article

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

Dihydroxyacetone synthase 1 promoter

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_pDAS2

Plasmid

#160917

Purpose

Dihydroxyacetone synthase 2 (DAS2) promoter module for episomal P. pastoris promoter shuffling

Depositor

Sylvestre Marillonnet

Article

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

Dihydroxyacetone synthase 2 promoter

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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TRPML2-CFP

Plasmid

#18831

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML2 (Mcoln2 Mouse)

Tags

CFP

Expression

Mammalian

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
pAGM9121_pAOX1

Plasmid

#160912

Purpose

Alcohol oxidase 1 (AOX1) promoter module for episomal P. pastoris promoter shuffling

Depositor

Sylvestre Marillonnet

Article

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

Alcohol oxidase 1 promoter

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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MXS_Tet

Plasmid

#62422

Purpose

MXS_chaining vector with Tet

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Tetracycline inducible promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MTK3_039

Plasmid

#123753

Purpose

Encodes mouse RelA as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

RelA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-28

Plasmid

#120007

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with 3xHA

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xHA

Use

Pcr template

Tags

3xHA

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge