We narrowed to 5,494 results for: SPI

1. EYFP-FKBP-N-WASP

   Plasmid

   #210302

   Purpose

   targeted actin nucleation

   Depositor

   Takanari Inoue

   Article

   Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

   Insert

   EYFP-FKBP-N-WASP
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. L2383-FRB-GPA-NLuc

   Plasmid

   #222902

   Purpose

   Positive control receptor chain for Nanoluciferase signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-20GS-NanoLuc
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMCSG7-10xHis-ecRNHI*

   Plasmid

   #219523

   Purpose

   bacterial expression of cysteine-free variant of the E. coli ribonuclease HI gene

   Depositor

   Arthur Palmer

   Article

   Bhattacharya et al J Biomol NMR. 2024 Jun 10. doi: 10.1007/

   Insert

   cysteine-free variant of the E. coli ribonuclease HI gene
   

   Use

   Tags

   N terminal 10xHis Tag

   Expression

   Bacterial

   Mutation

   Cysteine free variant

   Promoter

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA 3.1-nsp7

   Plasmid

   #201026

   Purpose

   Expresses SARS-CoV-2 nsp7 in mammalian.

   Depositor

   Sharon Rozovsky

   Article

   Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

   Insert

   non-structural protein 7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMSCV-IRES-GFP-Mouse-caspase-1/11a

   Plasmid

   #183391

   Purpose

   Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   CARD domain of mouse caspase-1 fused to catalytic domain of mouse caspase-11 (Casp1 Synthetic, Mouse)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   MESV

   Available since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNIR-Fb21

   Plasmid

   #184679

   Purpose

   NIR-Fb to spike-Cov-19 RBD

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   NIR-Fb21 (S Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMSCV-IRES-GFP-redesigned-human-Caspase-4_ANR261ENP_D315H_M318K_T352K

   Plasmid

   #183357

   Purpose

   Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-4 with ICE activity (CASP4 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   ANR261ENP, D315H, M318K, T352K

   Promoter

   MESV

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMSCV-IRES-GFP-Hybrid-caspase_canine-CARDs+mouse-Caspase-11-catalytic domain

   Plasmid

   #183396

   Purpose

   Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   CARD domains of canine caspase-1/4 fused to catalytic domain of mouse caspase-11
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   MESV

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMSCV-IRES-GFP-Hybrid-caspase_canine-CARDs+human-Caspase-4-catalytic domain

   Plasmid

   #183395

   Purpose

   Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   CARD domains of canine caspase-1/4 fused to catalytic domain of human caspase-4
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   MESV

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMSCV-IRES-GFP-Mouse-caspase-1/11b

    Plasmid

    #183392

    Purpose

    Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    CARD domain of mouse caspase-1 fused to mouse caspase-11 (Casp1 Synthetic, Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTF

    Plasmid

    #162784

    Purpose

    Protein expression in CHO cells - C-terminal FLAG and 10xhis tags are coded

    Depositor

    Ivan Vorobiev

    Article

    Kolesov et al Diagnostics (Basel). 2022 Feb 3;12(2). p

    Type

    Empty backbone

    Use

    Tags

    10xHis and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CHO Elongation factor 1A

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2-zfCSF3b

    Plasmid

    #168202

    Purpose

    Express zebrafish Csf3b, mRNA synthesis

    Depositor

    Petr Bartunek

    Article

    Stachura et al Blood. 2013 Dec 5;122(24):3918-28. doi:

    Insert

    Csf3b (csf3b Zebrafish)
    

    Use

    Vertebrate expression (zebrafish, chicken, xenopu…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCS2-zfCSF3a

    Plasmid

    #168201

    Purpose

    Express zebrafish Csf3a, mRNA synthesis

    Depositor

    Petr Bartunek

    Article

    Stachura et al Blood. 2013 Dec 5;122(24):3918-28. doi:

    Insert

    Csf3a (csf3a Zebrafish)
    

    Use

    Vertebrate expression (zebrafish, chicken, xenopu…

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of A27

    Promoter

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCSN071

    Plasmid

    #166729

    Purpose

    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae

    Depositor

    Rene Verwaal

    Article

    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

    Insert

    dCas12a-Mxi1-NLS
    

    Use

    CRISPR

    Tags

    Mxi1 and SV40 NLS

    Expression

    Yeast

    Mutation

    E925A

    Promoter

    S. cerevisiae TEF1

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCSN072

    Plasmid

    #166730

    Purpose

    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae

    Depositor

    Rene Verwaal

    Article

    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

    Insert

    dCas12a-NLS-Mxi1-NLS
    

    Use

    CRISPR

    Tags

    Mxi1 and SV40 NLS

    Expression

    Yeast

    Mutation

    E925A

    Promoter

    S. cerevisiae TEF1

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGBW-m4046774

    Plasmid

    #145574

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pGBW-m4046846

    Plasmid

    #145575

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pJS249

    Plasmid

    #134146

    Purpose

    Bacterial expression of human Ska1 microtubule binding domain (MTBD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    pHis-Precission-human Ska1 MTBD (SKA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJS263

    Plasmid

    #134141

    Purpose

    Bacterial expression of C. elegans Ska1 microtubule binding domain (MTBD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    Ska1 MTBD-His (Y106G6H.15 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIW087

    Plasmid

    #134128

    Purpose

    Bacterial expression of GFP-Ska1 and Ska2

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    His-GFP-human Ska1 ; human Ska2
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    K183A K184A

    Promoter

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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