3,637 results
-
Plasmid#217510PurposeKD Recombinase dependent conditional smGdP-10XV5 cassetteDepositorInsertKDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5
TagssmGdP-10XV5ExpressionInsectAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHD-DsRed-attP-w+
Plasmid#80898PurposeVector for generating dsDNA donors for HDR. Contains an attP docking site, the visible marker 3xP3-DsRed and GMR-white for identifying backbone incorporation.DepositorInsertsattP
LoxP-3xP3-DsRed-LoxP
GMR-white
UseHigh copy, amp resistancePromoter3xP3 and GMR::hsp70Available SinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
13XLexAop2 IVS ChrimsonR mCherry_tr
Plasmid#111548PurposeOptogenetics in Drosophila: LexAop2-induced expression of ChrimsonR mCherryDepositorInsertChrimsonR_mCherry_trafficked
ExpressionInsectMutationwtPromoterhsp70Available SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv::NG
Plasmid#138953PurposeUsed to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila using Gal4DepositorArticleInsertkkv (NEWENTRY Fly)
UseUas transgene vectorTagsNeon Green fused to C terminus of KkvExpressionInsectAvailable SinceNov. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWalium-ChtVis-Tomato
Plasmid#67756PurposeExpresses a fluorescent chitin reporter under Gal4 control in Drosophila.DepositorInsertdyl - chitin binding domain - tdTomato - dyl
Tagstd-tomatoExpressionInsectAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
W45A mutant of H3K9me3 biosensor
Plasmid#120808PurposeFRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
ExpressionMammalianMutationTryptophan 45 is mutated to Alanine 45 on HP1 dom…PromoterCMVAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
K9L mutant of H3K9me3 biosensor
Plasmid#120807PurposeFRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
ExpressionMammalianMutationlysine 9 is mutated to leucine 9 on histone H3PromoterCMVAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
w+attB
Plasmid#30326DepositorInsertphiC31 attB, hsp70::w
UseDrosophila transgenesisAvailable SinceJuly 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
Ac5-STABLE2-RFP-NLS-CycB(1-266)_GFP-E2F1(1-230)_neo
Plasmid#73164Purposemulti-cistronic Vector for expression for Fly_FUCCI probes in insect cellsDepositorTagsmRFP1, GFPExpressionInsectMutationCyclin B Fragment aa 1-266 & E2F1 Fragment aa…Available SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP
Plasmid#154786PurposeExpresses mCherry fused to hyperactive ADAR catalytic domain, control plasmid for HyperTRIBEDepositorInsertAdenosine deaminse (Adar Fly, Synthetic)
UseLentiviralTagsGFP, V5, and mCherryExpressionMammalianMutationSynonymized serine to prevent autoediting of ADAR…PromoterUBCAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
20XUAS IVS CsChrimson tdtomato_tr
Plasmid#111544PurposeOptogenetics in Drosophila: UAS-induced expression of Chrimson tdTomatoDepositorInsertChrimson_tdtomato_trafficked
ExpressionInsectMutationwtPromoterhsp70Available SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUAST attB Orco (Y478A)
Plasmid#73928PurposeUAS induced expression of Drosophila melanogaster Orco cDNA with 478 Tyrosine to Alanine amino acid changeDepositorAvailable SinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
RP156 pUAST EGFP:Gr21a
Plasmid#59524PurposeN-terminal fusion of EGFP to Drosophila melanogaster Gr21aDepositorAvailable SinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
RP125 pUAST EGFP:Orco
Plasmid#53441PurposeN-terminal fusion of EGFP to Drosophila melanogaster OrcoDepositorAvailable SinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCFD4d-U6-1:white1-U6-3:white1
Plasmid#84005PurposepCFD4d expresses white sgRNA-1 from a U6:3 promoter and the same white sgRNA-1 from a U6:1 promoterDepositorInsertswhite sgRNA-1
white sgRNA-1
UseCRISPRPromoterU6:1, U6:3, and dU6-1; dU6-3Available SinceJan. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
SCP1-CMVenh-luc
Plasmid#22216DepositorInsertSCP1 (super core promoter 1)-CMVenh luciferase
UseLuciferaseExpressionMammalianAvailable SinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAC-dCLK
Plasmid#33153DepositorAvailable SinceJan. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
RP185 pUAST EGFP:Or47b
Plasmid#59525PurposeN-terminal fusion of GFP to Drosophila melanogaster Or47bDepositorAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
RP132 pUAST EGFP:Or43a
Plasmid#59523PurposeN-terminal fusion of EGFP to Drosophila melanogaster Or43aDepositorAvailable SinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-EGFP-Vasa 463-661 MUT (F504E) (HK196)
Plasmid#206434PurposeVasa 463-661 expression in Schneider cells for imagingDepositorAvailable SinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only