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Showing: 41 - 80 of 358 results

  1. pCD/NL-BH∆1

    Plasmid
    #41791
    Purpose
    Packaging construct contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding Rev
    Depositor
    Jakob Reiser
    Article
    Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13
    Insert
    HIV-1 Gag/Pol and RRE sequences
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    41791-plasmid-map-sequence-id-171188

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  3. pCPRDEnv

    Plasmid
    #1732
    Purpose
    This is the FIV PPR based second generation packaging vector, part of the FELIX vector system from Nolan lab
    Depositor
    Garry Nolan
    Insert
    Gag-Pol and Rev
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 24, 2005
    Availability
    Academic Institutions and Nonprofits only
    1732-plasmid-map-sequence-id-326991

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  4. HIV-1-GFP ΔEnv

    Plasmid
    #217437
    Purpose
    HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu.
    Depositor
    Jarrod Johnson
    Article
    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.
    Insert
    gag/pol
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    217437-plasmid-map-sequence-id-426708

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  5. pCMVR8.74

    Plasmid
    #22036
    Purpose
    2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)
    Depositor
    Didier Trono
    Insert
    gag pol tat rev
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    22036-plasmid-map-sequence-id-339901

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  6. pfNL43-dGPE-EGFP

    Plasmid
    #36866
    Depositor
    Mario Chin
    Insert
    NL43-dGPE-EGFP
    Use
    Lentiviral and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    parts of Gag and Pol were deleted and EGFP insert…
    Promoter
    Available since
    Aug. 20, 2012
    Availability
    Academic Institutions and Nonprofits only
    36866-plasmid-map-sequence-id-46081

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  7. PJD1223

    Plasmid
    #160390
    Purpose
    Encodes L-A cDNA for curing L-A virus and Parent for pJD1223-NAT
    Depositor
    John McCusker
    Insert
    L-A Gag-Pol
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TRP1
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    160390-plasmid-map-sequence-id-314529

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  8. pCMV delta R8.2

    Plasmid
    #12263
    Purpose
    First-generation packaging plasmid. This plasmid contains HIV accessory genes. Please reach out to your biosafety officer for guidance.
    Depositor
    Didier Trono
    Insert
    HIV-1 GAG/POL, Tat and Rev
    Use
    Lentiviral; Packaging
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12263-plasmid-map-sequence-id-394974

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  9. HIV-1-GFP ΔEnv CA E45A

    Plasmid
    #217438
    Purpose
    HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with E45A mutation in CA).
    Depositor
    Jarrod Johnson
    Article
    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.
    Insert
    gag/pol
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    CA E45A
    Promoter
    Available since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    217438-plasmid-map-sequence-id-426692

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  10. HIV-1-GFP ΔEnv CA Q63/67A

    Plasmid
    #217439
    Purpose
    HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with Q63A,Q67A mutations in CA).
    Depositor
    Jarrod Johnson
    Article
    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.
    Insert
    gag/pol
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    CA Q63A,Q67A
    Promoter
    Available since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    217439-plasmid-map-sequence-id-426684

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  11. HIV-1-GFP ΔEnv CA M66I

    Plasmid
    #217440
    Purpose
    HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with M66I mutation in CA).
    Depositor
    Jarrod Johnson
    Article
    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.
    Insert
    gag/pol
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    CA M66I
    Promoter
    Available since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    217440-plasmid-map-sequence-id-426705

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  12. HIV-1-GFP ΔEnv CA Q67H/N74D

    Plasmid
    #217441
    Purpose
    HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef. Contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with Q67H,N74D mutations in CA).
    Depositor
    Jarrod Johnson
    Article
    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.
    Insert
    gag/pol
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    CA Q67H,N74D
    Promoter
    Available since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    217441-plasmid-map-sequence-id-426707

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  23. pLKO.1-TRC.mKO2_shHuR CDS

    Plasmid
    #110426
    Purpose
    TRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene and express monomeric Kusabira-Orange2.
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAVL1 HuR
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 (RNA Pol III)
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110426-plasmid-map-sequence-id-213399

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  26. pCD/NL-BH*deltavpu/RT-

    Plasmid
    #136985
    Purpose
    Second-generation lentiviral packaging plasmid lacking reverse transcriptase activity
    Depositor
    Jakob Reiser
    Article
    Zhang et al J Virol. 2004 Feb . 78(3):1219-29.
    Insert
    HIV-1 Gag, Pol (Integrase), Tat, Rev, Vpr, Vif
    Use
    Tags
    Expression
    Mammalian
    Mutation
    carries D110E mutation in the reverse transcripta…
    Promoter
    Available since
    Feb. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    136985-plasmid-map-sequence-id-271274

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  27. pcDNA3-CTE-Luc

    Plasmid
    #60881
    Purpose
    Reporter construct to study unspliced RNA export. Coding sequence of luciferase gene was sandwiched between 5' and 3' splice site sequences originating from the HIV gag/pol gene followed by CTE
    Depositor
    Vinay Nandicoori
    Article
    Rajanala et al PLoS One. 2012;7(1):e29921. doi: 10.1371
    Insert
    Luc-CTE
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    60881-plasmid-map-sequence-id-98231

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  29. HIV-1 LTR-gfp

    Plasmid
    #115809
    Purpose
    HIV-1 LTR driven reporter vector that retains complete LTRs, tat, and rev, but has a frameshift mutation in env, an ngfr reporter gene in place of nef, and gfp in place of gag, pol, vif, and vpr
    Depositor
    Jeremy Luban
    Article
    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.
    Insert
    gfp and ngfr
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    HIV1 LTR
    Available since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    115809-plasmid-map-sequence-id-232377

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  30. pUDP123

    Plasmid
    #107269
    Purpose
    pUDP123 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes OpADE2 and OpNIAD and Spcas9D147Y P411T in O. parapolymorpha (HH-gRNAOpADE2-HDV-linker-HH-gRNAOpNIAD-HDV)
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Insert
    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting OpADE2 and NIAD in O. parapolymorpha
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    107269-plasmid-map-sequence-id-208617

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  33. pLKO.1-blast shGAC

    Plasmid
    #110410
    Purpose
    shGAC (Target CCTCTGTTCTGTCAGAGTT), silence glutaminase isoform GAC, blasticidin selection.
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GLS glutaminase (GLS Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 (RNA Pol III)
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    110410-plasmid-map-sequence-id-218291

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  35. pUDP013

    Plasmid
    #103873
    Purpose
    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Ogataea (para)polymorpha
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Insert
    HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    103873-plasmid-map-sequence-id-199239

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  36. pCfb13217

    Plasmid
    #219892
    Purpose
    The base plasmid of TUNEYALI for TF27
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF27 (YALI1_E22758g) and homologous arm matching TF27
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    219892-plasmid-map-sequence-id-435519

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  37. pCfb13246

    Plasmid
    #219917
    Purpose
    The base plasmid of TUNEYALI for TF56
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF56 (YALI1_B06110g) and homologous arm matching TF56
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    219917-plasmid-map-sequence-id-435427

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  38. pUDP044

    Plasmid
    #101168
    Purpose
    pUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)
    Depositor
    Jean-Marc Daran
    Article
    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.
    Insert
    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101168-plasmid-map-sequence-id-199309

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  39. GFP-A96 ELP

    Plasmid
    #67856
    Purpose
    GFP Fluorescent Elastin-like Polypeptide(VPGAG) with 96 ELP repeats containing Alanine guest residue
    Depositor
    Andrew MacKay
    Insert
    GFP fused to ELP (VPGAG) repeated 96 times
    Use
    Tags
    GFP fused to N-terminal of ELP
    Expression
    Mammalian
    Mutation
    Promoter
    T7
    Available since
    Oct. 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    67856-plasmid-map-sequence-id-117813

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Showing: 41 - 80 of 358 results