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Showing: 51 - 100 of 8299 results

  2. PANK3

    Plasmid
    #32872
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PANK3 (PANK3 Human)
    Use
    Tags
    His tag (with Thrombin cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 10-370 only
    Promoter
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. pAN2981

    Plasmid
    #127246
    Purpose
    Asymmetric degronSwitchA_t8 fused to mCherry with KeyA-BFP
    Depositor
    Hana El-Samad
    Article
    Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586
    Insert
    pPGK-Key_A_e18-TagBFP-P2A-mCherry-asym_degronSwitch_A_t8-WPRE
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127246-plasmid-map-sequence-id-261344

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  6. PANK1

    Plasmid
    #25517
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PANK1 (PANK1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. PANK1

    Plasmid
    #32871
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PANK1 (PANK1 Human)
    Use
    Tags
    His tag (with Thrombin cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 231-597 only
    Promoter
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. pANY6

    Plasmid
    #112198
    Purpose
    This plasmid is used for efficient cloning of DNA, efficient expression of protein in E. coli, and ELP-based protein purification.
    Depositor
    Yingfeng An
    Article
    Gao et al J Agric Food Chem. 2018 Jun 20;66(24):61
    Type
    Empty backbone
    Use
    Tags
    ELP-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    112198-plasmid-map-sequence-id-218116

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  14. PANK3

    Plasmid
    #25518
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PANK3 (PANK3 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. pAN1735

    Plasmid
    #127204
    Purpose
    Dual inducible expression of SynTF-mScarlet-degronSwitchA and KeyA-BFP-NLS
    Depositor
    Hana El-Samad
    Article
    Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586
    Insert
    pGal1-SynTF-mScarlet-degronSwitch_A_t12-tSSA1-pZ3-key_A_e18-mTagBFP2-NLS(SV40)-tENO1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    127204-plasmid-map-sequence-id-250425

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  16. pAN1779

    Plasmid
    #127165
    Purpose
    Vector for integrating the process (pGAL1 expression of Z3PM and mKate2 both fused to psd) and the circuit output (pZ3-Venus-cODC)
    Depositor
    Hana El-Samad
    Article
    Ng et al Nature. 2019 Jul 24. pii: 10.1038/s41586
    Insert
    pGAL1-Z3PM-psd-tADH1-pGAL1-mKate2-psd-tPGK1-pZ3-Venus-cODC-tTDH1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    127165-plasmid-map-sequence-id-250015

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  17. pANY3

    Plasmid
    #112197
    Purpose
    This plasmid is used for efficient cloning of DNA and temperature-induced expression of protein in E. coli.
    Depositor
    Yingfeng An
    Article
    Gao et al J Agric Food Chem. 2018 Jun 20;66(24):61
    Type
    Empty backbone
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112197-plasmid-map-sequence-id-218115

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  18. pAN1935

    Plasmid
    #127203
    Purpose
    Constitutive expression of Venus-degronSwitchA and mScarlet-degronSwitchC
    Depositor
    Hana El-Samad
    Article
    Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586
    Insert
    pTEF1-Venus-degronSwitch_A_t12-tENO2-pTEF2-mScarlet-Linker-degronSwitch_C-tPGK1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    127203-plasmid-map-sequence-id-250001

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  19. pAN1232

    Plasmid
    #127164
    Purpose
    Vector for integrating the controller (constitutive expression of GEM-degronSwitch and pZ3 expression of key)
    Depositor
    Hana El-Samad
    Article
    Ng et al Nature. 2019 Jul 24. pii: 10.1038/s41586
    Insert
    pRPL18B-GEM-degronSwitchA-tENO2-pZ3-keyA-mTurquoise2-NLS(SV40)-tTDH1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    127164-plasmid-map-sequence-id-250325

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  23. pAN68

    Plasmid
    #164525
    Purpose
    Retroviral expression of CENP-R uORF-1
    Depositor
    Iain Cheeseman
    Insert
    CENP-R uORF-1 (ITGB3BP Human)
    Use
    Retroviral
    Tags
    -6xhis-precission-GFP
    Expression
    Mutation
    Promoter
    Available since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    164525-plasmid-map-sequence-id-328004

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  25. pAN007

    Plasmid
    #220048
    Purpose
    Reporter plasmid that encodes for the GFP fused to an N-terminal flag-HA epitope. A stop codon is inserted between the epitope tag and the gfp sequence to terminate GFP translation.
    Depositor
    Blake Wiedenheft
    Article
    Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1
    Insert
    flag-HA-stop-GFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220048-plasmid-map-sequence-id-434404

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  26. pAN013

    Plasmid
    #220050
    Purpose
    Reporter plasmid that expresses GFP and firefly luciferase linked with a viral 2A peptide. A stop codon is inserted at the 3'-end of the gfp gene, which permits GFP, but not luciferase expression.
    Depositor
    Blake Wiedenheft
    Article
    Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1
    Insert
    GFP-stop-fLuc
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220050-plasmid-map-sequence-id-434403

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  28. pAN057

    Plasmid
    #220053
    Purpose
    NMD-reporter plasmid that expresses a a firefly luciferase gene fused to exons 22-27 of the CFTR gene and a synthetic intron (c.3846G>A mutation; W1282X).
    Depositor
    Blake Wiedenheft
    Article
    Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1
    Insert
    fLuc-CFTR(W1282X, exons 22-27) (CFTR Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    c.3846G>A mutation
    Promoter
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220053-plasmid-map-sequence-id-434400

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  32. pAN052

    Plasmid
    #220046
    Purpose
    Mammalian expression of type III-A Csm complex from Streptococcus thermophilus without NLS tags
    Depositor
    Blake Wiedenheft
    Article
    Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1
    Insert
    FLAG-Csm1; FLAG-Csm2; FLAG-Csm3; FLAG-Csm4; FLAG-Csm5; FLAG-Cas6; crRNA; GFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220046-plasmid-map-sequence-id-434396

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  34. pANG03

    Plasmid
    #178085
    Purpose
    Tetracycline resistant backbone for in vivo Gateway reaction. Plasmid contains gateway cassette with restriction sites to add promoters or C and N Terminal tags. Compatible with pANG02.
    Depositor
    Sören Abel
    Article
    Gillman et al Plasmid. 2021 Nov;118:102608. doi: 10.10
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    NA
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    178085-plasmid-map-sequence-id-349913

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  35. pANG01

    Plasmid
    #178083
    Purpose
    Carbenicillin resistant backbone for in vivo Gateway reaction. Plasmid contains gateway cassette with restriction sites to add promoters or C and N Terminal tags. Compatible with pANG02.
    Depositor
    Sören Abel
    Article
    Gillman et al Plasmid. 2021 Nov;118:102608. doi: 10.10
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    NA
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    178083-plasmid-map-sequence-id-349911

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  36. pANG02

    Plasmid
    #178084
    Purpose
    Gentamycin resistant backbone for in vivo Gateway reaction. Plasmid contains gateway cassette with restriction sites to add promoters or C and N Terminal tags. Compatible with pANG01 or pANG03.
    Depositor
    Sören Abel
    Article
    Gillman et al Plasmid. 2021 Nov;118:102608. doi: 10.10
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    NA
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    178084-plasmid-map-sequence-id-349910

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  38. pANY8

    Plasmid
    #164900
    Purpose
    Containing His-ELP-intein multifunctional tag for protein purification by combining Ni-NTA-based and ELP-based strategy with a specially designed purification protocol.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Use
    Tags
    His-ELP-Intein-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    164900-plasmid-map-sequence-id-330483

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  39. pANY4

    Plasmid
    #164897
    Purpose
    Two valuable constructs for either IPTG-induced or temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    164897-plasmid-map-sequence-id-327986

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  44. pAN1646

    Plasmid
    #127205
    Purpose
    pTet expression of Venus, Dual inducible expression of dCas9-VP64-mScarlet-degronSwitchA and KeyA-BFP-NLS
    Depositor
    Hana El-Samad
    Article
    Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586
    Insert
    p7x_tet-Venus-tADH1-pGal1-dCas9-VP64-Linker-mScarlet-Linker-degronSwitch_A_t12-tPGK1-pZ3-key_A_e18-mTagBFP2-NLS(SV40)-tSSA1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    127205-plasmid-map-sequence-id-250026

    Enlarge

  45. pAN1777

    Plasmid
    #127206
    Purpose
    Dual inducible expression of SynTF-mScarlet-nesSwitchA and KeyA-BFP-NLS
    Depositor
    Hana El-Samad
    Article
    Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586
    Insert
    pGal1-NLS(activity4)-SynTF-mScarlet-1_nesSwitch_A_t9-tSSA1-pZ3-key_A_e18-mTagBFP2-tENO1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    127206-plasmid-map-sequence-id-249999

    Enlarge

  46. pAN289

    Plasmid
    #164524
    Purpose
    Retroviral expression of CENP-R uORF-1
    Depositor
    Iain Cheeseman
    Insert
    CENP-R uORF-1 (ITGB3BP Human)
    Use
    Retroviral
    Tags
    eGFP-Tev-S
    Expression
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    164524-plasmid-map-sequence-id-328003

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Showing: 51 - 100 of 8299 results