We narrowed to 621 results for: 1302
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Plasmid#1302DepositorInsertHsp104 F130W (HSP104 Budding Yeast)
Tags10X His tagExpressionBacterialMutationChanged Phe 130 to TrpAvailable SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only
These results may also be relevant.
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pJPF0190
Plasmid#130268PurposePiggybac vector for the expression of mRuby2-fused human PRKACADepositorAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNH12 (pmyo-2::MacQ::mCitrine)
Plasmid#130274PurposeExpresses the eFRET-based voltage sensor MacQ::mCitrine in the pharynx of C. elegans.DepositorInsertMacQ::mCitrine
TagsmCitrineExpressionWormAvailable SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
G31, Cdc20-AID tagging
Plasmid#130269PurposeTagging the Saccharomyces cerevisiae Cdc20 gene with a auxin-degron tag, via homologous recombinationDepositorInsertCdc20/AID(71-114)-tCYC1-NatMX (CDC20 Synthetic, Budding Yeast)
TagsAID(71-114)ExpressionYeastPromoterNative promoterAvailable SinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-Act5c
Plasmid#130278PurposeDrosophila expression of sgRNA that targets Actin5c CDS close to stop codon. Positive control sgRNA for CRISPaint knock-ins in cultured cells.DepositorAvailable SinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK_Tol2_TRE_Zeo
Plasmid#130263PurposeTol2 doxycycline inducible expression vectorDepositorTypeEmpty backboneUseTol2 transposon, doxycycline inducibleExpressionMammalianPromoterTRE PromoterAvailable SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTV001
Plasmid#130265PurposeEatA expression plasmid containing 8His encoding region in permissive site of eatA geneDepositorInserteatA::His8
Tagspolyhistidine (internal)ExpressionBacterialMutation8His polyhistidine encoding region and XhoI site …PromoteraraBADAvailable SinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Donor/Reporter-COL4A5
Plasmid#130280PurposeCOL4A5 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A5 donor templateDepositorInsertmCherry-eGFP
UseAAVPromoterCMVAvailable SinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-EMMI-007 Esp3I//(A)-AarI-SacB-AarI-(Z)//Esp3I
Plasmid#130215PurposeEMMA Kit # 1000000119 extensionDepositorInsertSacB
UseSynthetic BiologyAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
G30, Cdc20-mCherry-AID tagging
Plasmid#130270PurposeTagging the Saccharomyces cerevisiae Cdc20 gene with a mCherry and auxin-degron tag, via homologous recombinationDepositorInsertCdc20/mCherry-AID(71-114)-tCYC1-NatMX (CDC20 Synthetic, Budding Yeast)
TagsAID(71-114) and mCherryExpressionYeastPromoterNative promoterAvailable SinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-029 Vimentin-mVenus//BsaI
Plasmid#130210PurposeEMMA Kit # 1000000119 extensionDepositorInsertVimentin-mVenus
UseSynthetic BiologyAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-EMMI-008 Esp3I//(A)-BsaI-SacB-BsaI-(Z)//Esp3I
Plasmid#130216PurposeEMMA Kit # 1000000119 extensionDepositorInsertSacB
UseSynthetic BiologyAvailable SinceFeb. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Donor/Reporter-COL4A3
Plasmid#130279PurposeCOL4A3 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A3 donor templateDepositorInsertmCherry-eGFP
UseAAVPromoterCMVAvailable SinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV_COL4A3_self-cleavingCas9
Plasmid#130281PurposeExpresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A3 sgRNA targets to induce its self-cleaving and inactivationDepositorInsertspCas9
UseAAVPromoterCMVAvailable SinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV_COL4A5_self-cleavingCas9
Plasmid#130282PurposeExpresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A5 sgRNA targets to induce its self-cleaving and inactivationDepositorInsertspCas9
UseAAVPromoterCMVAvailable SinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-EMMI-021 IRES-D[C333G](H-I)//Esp3I
Plasmid#130223PurposeEMMA Kit # 1000000119 extensionDepositorInsertIRES-D[C333G]
UseSynthetic BiologyMutationC333GAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-EMMI-023 IRES-F[C336A](H-I)//Esp3I
Plasmid#130225PurposeEMMA Kit # 1000000119 extensionDepositorInsertIRES-F[C336A]
UseSynthetic BiologyMutationC336AAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-EMMI-024 Connector(D-G)//Esp3I
Plasmid#130226PurposeEMMA Kit # 1000000119 extensionDepositorInsertartificial connector
UseSynthetic BiologyAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-EMMI-025 eGFP(I-J)//Esp3I
Plasmid#130227PurposeEMMA Kit # 1000000119 extensionDepositorInserteGFP
UseSynthetic BiologyAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-EMMI-026 mCherry(I-J)//Esp3I
Plasmid#130228PurposeEMMA Kit # 1000000119 extensionDepositorInsertmCherry
UseSynthetic BiologyAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only