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pMSCV_PIG_3XHA-Ago1

Plasmid

#170916

Purpose

Expresses 3X-HA-AGO1

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

Ago1 (Ago1 Mouse)

Use

Tags

3X-HA

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHA

Plasmid

#136659

Purpose

Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA

Depositor

Yannick Doyon

Article

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD9:TH1477 chimera
sgRNA for St1Cas9
APOBEC-1 (APOBEC1 Human)
UGI
HA tag

Use

CRISPR and Synthetic Biology

Tags

2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

Expression

Mammalian

Mutation

Promoter

CAG and U6

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLV_PGK_3xHA-LTB4R

Plasmid

#208091

Purpose

Mammalian expression of the wild-type human LTB4 receptor LTB4R with an N-terminal 3x HA tag.

Depositor

Sean R Collins

Article

Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

Insert

3xHA-LTB4R (LTB4R Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

none

Promoter

PGK

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO_ORF18_TurboID_3xHA

Plasmid

#200017

Purpose

Expresses KSHV protein ORF18 fused at the C-terminal to TurboID-3xHA

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF18

Use

Tags

TurboID-3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO_ORF30_TurboID_3xHA

Plasmid

#200018

Purpose

Expresses KSHV protein ORF30 fused at the C-terminal to TurboID-3xHA

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF30

Use

Tags

TurboID-3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO_ORF31_TurboID_3xHA

Plasmid

#200019

Purpose

Expresses KSHV protein ORF31 fused at the C-terminal to TurboID-3xHA

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF31

Use

Tags

TurboID-3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO_ORF66_TurboID_3xHA

Plasmid

#200020

Purpose

Expresses KSHV protein ORF66 fused at the C-terminal to TurboID-3xHA

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF66

Use

Tags

TurboID-3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO_3xHA_TurboID_ORF34

Plasmid

#200021

Purpose

Expresses KSHV protein ORF34 fused at the N-terminal to 3xHA-TurboID

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF34

Use

Tags

3xHA-TurboID

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMG18311_2xUGI_3xHA

Plasmid

#136657

Purpose

Expresses St1BE4max-LMG18311 in mammalian cells along with its U6-driven sgRNA

Depositor

Yannick Doyon

Article

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD9:LMG18311 chimera
sgRNA for St1Cas9
APOBEC-1 (APOBEC1 Human)
UGI
HA tag

Use

CRISPR and Synthetic Biology

Tags

2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

Expression

Mammalian

Mutation

Promoter

CAG and U6

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_CNRZ1066_2xUGI_3xHA

Plasmid

#136654

Purpose

Expresses St1BE4max-CNRZ1066 in mammalian cells along with its U6-driven sgRNA

Depositor

Yannick Doyon

Article

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD9:CNRZ1066 chimera
sgRNA for St1Cas9
APOBEC-1 (APOBEC1 Human)
UGI
HA tag

Use

CRISPR and Synthetic Biology

Tags

2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

Expression

Mammalian

Mutation

Promoter

CAG and U6

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLJC5-Tmem192-3xHA

Plasmid

#102930

Purpose

Express a lysosomal Tag

Depositor

David Sabatini

Article

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

Tmem192 (TMEM192 Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

ubc

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pLJC6-3XHA-TMEM192

Plasmid

#104434

Purpose

Expresses 3XHA-TMEM192 in mammalian cells

Depositor

David Sabatini

Article

Wyant et al Cell. 2017 Oct 19;171(3):642-654.e12. do

Insert

TMEM192 (TMEM192 Human)

Use

Lentiviral

Tags

3XHA

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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pLV_3XHA-ATP6V1B2-mNeonGreen

Plasmid

#228865

Purpose

Stable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA and C-terminal mNeonGreen

Depositor

Wilhelm Palm

Article

Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

Insert

ATP6V1B2 (ATP6V1B2 Human)

Use

Lentiviral

Tags

3xHA and mNeonGreen

Expression

Mutation

Promoter

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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3xHA-miniTurbo-NLS_pCDNA3

Plasmid

#107172

Purpose

expresses 3xHA-tagged miniTurbo in the mammalian nucleus

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Use

Tags

Expression

Mammalian

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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3xHA-TurboID pRetrox

Plasmid

#155203

Purpose

For doxycycline-inducible expression of 3xHA-TurboID on N-terminus of bait protein

Depositor

Kyle Roux

Article

May et al Cells. 2020 Apr 25;9(5). pii: cells90510

Insert

3xHA-TurboID

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

tight TRE promotor

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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3xHA-TurboID-NLS_pCDNA3

Plasmid

#107171

Purpose

expresses 3xHA-tagged TurboID in the mammalian nucleus

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Use

Tags

Expression

Mammalian

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3XHA-p53

Plasmid

#196267

Purpose

Mammalian expression of 3xHA tagged wild-type p53

Depositor

Laura Attardi

Article

Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

Insert

p53 (Trp53 Mouse)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA-3xHA-hTERT

Plasmid

#51637

Purpose

Mammalian expression of 3xHA tagged human TERT, N-terminal.

Depositor

Steven Artandi

Article

Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

Insert

TERT (TERT Human)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 16, 2014

Availability

Academic Institutions and Nonprofits only

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DRD2-3xHA/pCDNA3.1

Plasmid

#196940

Purpose

Plasmid expressing an antigen targeting the D2 dopamine receptor

Depositor

James Trimmer

Insert

DRD2 (DRD2 Human)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-C-3xHA-Puro

Plasmid

#207729

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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