We narrowed to 2,854 results for: 623

1. pET-(-30)GFP-9xGGS-Cre-6xHis

   Plasmid

   #62372

   Purpose

   Expression of (-30)GFP-9xGGS-Cre-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   (-30)GFP-9xGGS-Cre-6xHis
   

   Use

   Tags

   (-30)GFP and 6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pACYC-GST

   Plasmid

   #62329

   Purpose

   E. coli pACYC based expression vector with N-terminal TEV cleavable GST-tag

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Use

   Tags

   Glutathione S-transferase (GST) tag and TEV prote…

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lac0

   Available since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFBOH-MHL

   Plasmid

   #62304

   Purpose

   It is a donor vector for generation of recombinant baculovirus by site-specific transposition in an E. coli host.

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Use

   Baculovirus expression

   Tags

   6xHis and TEV cleavage site

   Expression

   Mutation

   Promoter

   polyhedrin

   Available since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTRC-LIC

   Plasmid

   #62343

   Purpose

   Used for tac promoter regulated high-level expression of cloned, untagged genes in E. Coli.

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter from pKK233-2

   Available since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJZC588

   Plasmid

   #62315

   Purpose

   sgRNA with 2x MS2 (wt+f6) for yeast cells

   Depositor

   Stanley Qi, Wendell Lim

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Insert

   sgRNA + 2x MS2 (wt+f6) binding module
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBSKΔB-CAG-MCP-tdiRFP670-IRES-Neo

   Plasmid

   #62345

   Purpose

   Expresses MCP-tdiRFP670 in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

   Insert

   MCP-tdiRFP670
   

   Use

   Tags

   HA and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJW1507

   Plasmid

   #62361

   Purpose

   pFA6a -mVenus-BirA::HIS5

   Depositor

   Jonathan Weissman

   Article

   Jan et al Science. 2014 Nov 7;346(6210):1257521. d

   Insert

   c-terminal mVenus-BirA tagging
   

   Use

   Yeast c-terminal tagging vector

   Tags

   mVenus-BirA

   Expression

   Mutation

   L232H mutation compared to standard mVenus

   Promoter

   Available since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJZC603

   Plasmid

   #62317

   Purpose

   sgRNA with 2x PP7 for yeast cells

   Depositor

   Stanley Qi, Wendell Lim

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Insert

   sgRNA + 2x PP7 RNA binding module
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJZC33

   Plasmid

   #62330

   Purpose

   sgRNA + 2x MS2 with MCP-VP64 effector for mammalian cells

   Depositor

   Stanley Qi, Wendell Lim

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Inserts

   sgRNA + 2x MS2 binding module
   MCP-VP64
   

   Use

   Lentiviral

   Tags

   VP64

   Expression

   Mammalian

   Mutation

   Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

   Promoter

   CMV and U6

   Available since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Actin binding domain of Ezrin

    Plasmid

    #62381

    Purpose

    Expresses the actin binding domain of ezrin. Amino acids 552-586.

    Depositor

    Guillaume Charras

    Article

    Fritzsche et al Biophys J. 2014 Jan 21;106(2):343-53. do

    Insert

    Actin binding domain of ezrin (EZR Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains aa's 552-586

    Promoter

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAN-NOR

    Plasmid

    #62306

    Purpose

    synthetic circuit: NOR gate

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Inserts

    A2NT sgRNA
    A2NT sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PPhlF and pBAD

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAN-OR

    Plasmid

    #62307

    Purpose

    synthetic circuit: OR gate

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Inserts

    A2NT sgRNA
    A4NT sgRNA
    A2NT sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PA2, PPhlF, and pBAD

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET32a-LIC

    Plasmid

    #62310

    Purpose

    E. coli expression vector with N-terminal Trx-tag, 6X His-tag, thrombin site, S-tag, and TEV

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6xHis, S-tag, and Trx-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJZC545

    Plasmid

    #62313

    Purpose

    sgRNA with 1x MS2 for yeast cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + 1x MS2 binding module
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJZC48

    Plasmid

    #62336

    Purpose

    sgRNA + 1x COM with COM-VP64 effector for mammalian cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 1x COM binding module
    COM-VP64
    

    Use

    Lentiviral

    Tags

    VP64

    Expression

    Mammalian

    Mutation

    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

    Promoter

    CMV and U6

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p28BIOH-LIC

    Plasmid

    #62352

    Purpose

    E. coli expression vector with N-terminal AviTag and a C-terminal Hexa-HIS tag

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6xHis and AviTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTV-Oct4-TK-HMS

    Plasmid

    #62351

    Purpose

    Mouse Oct4 targeting vector

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

    Insert

    TK-Hyg-24xMS2
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJW1234

    Plasmid

    #62367

    Purpose

    pRS305 pPGK1-BirA::LEU2

    Depositor

    Jonathan Weissman

    Article

    Jan et al Science. 2014 Nov 7;346(6210):1257521. d

    Insert

    BirA (birA )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBSKΔB-TRE-dCas9-EGFP

    Plasmid

    #62347

    Purpose

    Plasmid for tet-inducible expression of dCas9-EGFP in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

    Insert

    dCas9-EGFP
    

    Use

    CRISPR

    Tags

    HA and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pENTR L1-vas2-Cre-L4

    Plasmid

    #62301

    Purpose

    Cre recombinase-expressing helper plasmid for Anopheles gene engineering, vasa promoter

    Depositor

    Eric Marois

    Article

    Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

    Insert

    Cre recombinase under control of vasa promoter and terminator
    

    Use

    Tags

    HA and SV40 NLS

    Expression

    Insect

    Mutation

    Promoter

    vasa

    Available since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge