Skip to main content
Addgene
Showing: 21 - 40 of 13586 results

  1. pET-21a(+)-BMTU UDG-6xHis

    Plasmid
    #172197
    Purpose
    expresses His-tagged uracil-DNA-glycosylase enzyme from marine bacterium BMTU 3346 for the purpose of cross contamination prevention in nucleic acid amplification reactions
    Depositor
    Andrea Pauli
    Insert
    BMTU UDG
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    172197-plasmid-map-sequence-id-345439

    Enlarge

  2. pUb-tdTomato-LP-Neo-LP

    Plasmid
    #231509
    Purpose
    Fluorescent reporter for tdTomato expression in Acanthamoeba via the Cre/loxP system
    Depositor
    Yeonchul Hong
    Insert
    tdTomato fluorescent protein
    Use
    Unspecified; Acanthamoeba castellanii neff strain
    Tags
    Expression
    Mutation
    Promoter
    Acanthamoeba polyubiquitin
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    231509-plasmid-map-sequence-id-462659

    Enlarge

  3. pGAPDH-Cre-HYG

    Plasmid
    #231510
    Purpose
    Expresses Cre recombinase in Acanthamoeba
    Depositor
    Yeonchul Hong
    Insert
    Cre recombinase
    Use
    Unspecified; Acanthamoeba castellanii neff strain
    Tags
    EGFP and SV40 NLS
    Expression
    Mutation
    Promoter
    Acanthamoeba GAPDH
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    231510-plasmid-map-sequence-id-462631

    Enlarge

  4. FB027

    Plasmid
    #119712
    Purpose
    Multipartite assembly obtained by combining FB parts FB026+FB003 into pDGB3omega1. Used for fluorescent tagging of fungi (Hyg resistance +YFP) according to FungalBraid modular DNA assembly for ATMT
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    PgpdA::YFP::TtrpC(←)-PtrpC::hph::Ttub (→)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    119712-plasmid-map-sequence-id-236968

    Enlarge

  5. FB012

    Plasmid
    #119709
    Purpose
    Complete TU for hygromicin resistance in fungi, domesticated into pUPD2 with AATG/GCTT barcodes. Used for gene KO with dual selection, according to FungalBraid modular DNA assembly for ATMT
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    PtrpC:hph:Ttub
    Use
    Synthetic Biology; Domestication of dna parts for…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119709-plasmid-map-sequence-id-237149

    Enlarge

  6. FB039

    Plasmid
    #119715
    Purpose
    Multipartite assembly obtained by combining FBparts FB026+FB009 into pDGB3omega1. Used for fluorescent tagging of fungi (G418 resistance + YFP), according to FungalBraid modular DNA assembly for ATMT.
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    PgpdA::YFP::TtrpC(←)-PtrpC:nptII::Ttub (→)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119715-plasmid-map-sequence-id-245416

    Enlarge

  7. FB010

    Plasmid
    #119708
    Purpose
    Transcriptional Unit (TU) for hygromicin resistance in fungi obtained by combining FB001+GB0211+FB002 into pDGB3alpha1. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    PtrpC:hph:Ttub
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    119708-plasmid-map-sequence-id-236964

    Enlarge

  8. FB003

    Plasmid
    #119677
    Purpose
    Transcriptional Unit (TU) for hygromicin resistance in fungi obtained by combining FB001+GB0211+FB002 into pDGB3alpha2. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    PtrpC:hph:Ttub
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    119677-plasmid-map-sequence-id-238439

    Enlarge

  9. FB009

    Plasmid
    #119707
    Purpose
    Transcriptional Unit (TU) for geneticin (G418) resistance in fungi obtained by combining FB001+FB005+FB002 into pDGB3alpha2. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    PtrpC::nptII::Ttub
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    119707-plasmid-map-sequence-id-236967

    Enlarge

  10. MLM3636

    Plasmid
    #43860
    Purpose
    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoter
    Depositor
    Keith Joung
    Insert
    None
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    March 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    43860-plasmid-map-sequence-id-194368

    Enlarge

  11. FB007

    Plasmid
    #119705
    Purpose
    Promoter gpdA from Aspergillus nidulans domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Promoter gpdA
    Use
    Synthetic Biology; Domestication of dna parts for…
    Tags
    Expression
    Mutation
    Promoter
    gpdA
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119705-plasmid-map-sequence-id-237147

    Enlarge

  12. FB029

    Plasmid
    #119713
    Purpose
    Promoter paf from Penicillium chrysogenum domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Promoter paf
    Use
    Synthetic Biology; Domestication of dna parts for…
    Tags
    Expression
    Mutation
    Promoter
    Promoter paf
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119713-plasmid-map-sequence-id-237611

    Enlarge

  13. FB030

    Plasmid
    #119714
    Purpose
    Terminator paf from P. chrysogenum domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Terminator paf
    Use
    Synthetic Biology; Domestication of dna parts for…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119714-plasmid-map-sequence-id-237150

    Enlarge

  14. FB005

    Plasmid
    #119678
    Purpose
    Coding sequence nptII from E. coli domesticated into pUPD2 with AATG/GCTT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    geneticin resistance marker
    Use
    Synthetic Biology; Domestication of dna parts for…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119678-plasmid-map-sequence-id-237143

    Enlarge

  15. FB001

    Plasmid
    #119057
    Purpose
    Promoter trpC from Aspergillus nidulans domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Promoter trpC
    Use
    Synthetic Biology; Domestication of dna parts for…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119057-plasmid-map-sequence-id-237151

    Enlarge

  16. FB002

    Plasmid
    #119676
    Purpose
    Terminator tub from Neurospora crassa domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Terminator tub
    Use
    Synthetic Biology; Domestication of dna parts for…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119676-plasmid-map-sequence-id-237306

    Enlarge

  17. FB008

    Plasmid
    #119706
    Purpose
    Terminator trpC from Aspergillus nidulans domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Terminator trpC
    Use
    Synthetic Biology; Domestication of dna parts for…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119706-plasmid-map-sequence-id-237146

    Enlarge

  18. FB026

    Plasmid
    #119711
    Purpose
    Transcriptional Unit (TU) for YFP expression, obtained by combining FB/GBparts FB007+GB0053+FB008 into pDGB3alpha1R. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Promoter gpdA:YFP coding sequence:Terminator trpC
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119711-plasmid-map-sequence-id-236966

    Enlarge

  19. pLM-AMA15.0 Cas9

    Plasmid
    #138944
    Purpose
    Fungal AMA1 plasmid with sp-Cas9, ribozymes based "plug-and-play" sgRNA transcription unit, Terbinafine and Phleomycin selection markers
    Depositor
    Arnold Driessen
    Article
    Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10
    Insert
    p40S:sp_Cas9_2xNLS; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
    Use
    CRISPR and Synthetic Biology; Ama1 autonomously r…
    Tags
    NLS
    Expression
    Mutation
    Promoter
    p40S (AN0465) Aspergillus nidulans
    Available since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    138944-plasmid-map-sequence-id-364383

    Enlarge

  20. FB013

    Plasmid
    #119710
    Purpose
    TU of the HSVtk negative marker for F2dU domesticated into pUPD2 with GGAG/TACT barcodes (reverse orientation). Used for gene KO with dual selec. According to FungalBraid modular DNA assembly for ATMT
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Negative selection marker HSVtk (Pgpda:HSVtk:Ttub)
    Use
    Synthetic Biology; Domestication of dna parts for…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119710-plasmid-map-sequence-id-237145

    Enlarge

Showing: 21 - 40 of 13586 results