We narrowed to 2,116 results for: dcas9

1. pTDH3-dCas9

   Plasmid

   #46920

   Purpose

   Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS controlled by TDH3 promoter

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH3

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSET-dCas9

   Plasmid

   #110183

   Purpose

   Expression of the dCas9 gene in Streptomyces

   Depositor

   Yinhua Lu

   Article

   Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

   Insert

   dCas9
   

   Use

   CRISPR; I

   Tags

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   ermE*p

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. DNMT3L-dCas9

   Plasmid

   #126587

   Purpose

   DNMT3L fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):

   Insert

   DNMT3L (DNMT3L Human)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-DNMT3L-dCas9-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. VP64-dCas9

   Plasmid

   #177171

   Purpose

   VP64 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s

   Insert

   VP64-dCas9(D10A, H840A)
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xFLag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAW91.dCas9

   Plasmid

   #104372

   Purpose

   Expresses dCas9

   Depositor

   Richard Young

   Article

   Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. dCas9-DNMT3L

   Plasmid

   #126588

   Purpose

   DNMT3L fused to C-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):

   Insert

   DNMT3L (DNMT3L Human)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-dCas9-DNMT3L-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXFokI-dCas9

   Plasmid

   #60901

   Purpose

   Dual Expression Vector for FokI-dCas9 and gRNA

   Depositor

   Bruce Conklin

   Article

   Miyaoka et al Sci Rep. 2016 Mar 31;6:23549. doi: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. dCas9-CRY2Clust

   Plasmid

   #200969

   Purpose

   induction of phase-separated condensate by CRY2Clust

   Depositor

   Jian Xu

   Article

   Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

   Insert

   CRY2Clust
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. dCas9-FCPF

   Plasmid

   #220131

   Purpose

   Expresses dCas9-FCPF in mammalian cells

   Depositor

   Xinlai Cheng

   Article

   Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

   Insert

   dCas9-FCPF (cas9 Synthetic, S. pyogenes)
   

   Use

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMRS-dCas9

    Plasmid

    #220193

    Purpose

    mini-Tn7 dCas9 expression vector

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Pseudomonas aeruginosa codon optimized

    Promoter

    promoterless

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. LLP774_dCas9-Spy-Snoop-Sunx5-Avi-Tag-BFP (dCas9-SSSavi-BFP)

    Plasmid

    #211767

    Purpose

    dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), with BFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    dCas9, Spy, Snoop, aGCN4, AviTags
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    dCas9 D10A and H840A

    Promoter

    pGK and pEF1a

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBbdCas9S

    Plasmid

    #149638

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, empty

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB_tre_dCas9_KRAB

    Plasmid

    #126030

    Purpose

    PiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusion

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Inserts

    dCas9 fusion with KRAB
    Hygromycin resistance
    

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    EF1 alpha and TRE

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB_tre_dCas9_VP160

    Plasmid

    #126031

    Purpose

    PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Inserts

    dCas9 fusion with VP160
    Hygromycin resistance
    

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    EF1 alpha and TRE

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pREDCas9

    Plasmid

    #71541

    Purpose

    Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

    Depositor

    Tao Chen

    Article

    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

    Inserts

    Cas9
    lambda red genes
    plasmid curing system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS42H_PTEF1_dCas9_TCYC1

    Plasmid

    #177155

    Purpose

    Expresses dCas9 in yeast cells

    Depositor

    Gautam Dantas

    Article

    Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

    Insert

    Nuclease-null Cas9
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 promoter

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEF_dCas9

    Plasmid

    #68416

    Purpose

    Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    human EF1[alpha]

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pdCas9

    Plasmid

    #46569

    Purpose

    Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.

    Depositor

    Luciano Marraffini

    Article

    Bikard et al Nucleic Acids Res. 2013 Jun 12.

    Inserts

    tracrRNA
    dcas9
    CRISPR array
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTPGI_dCas9_VP64

    Plasmid

    #49013

    Purpose

    encodes yeast-optimized dCas9_VP64 synthetic transcription factor

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    dCas9_VP64 (codon-optimized for expression in S. cerevisiae)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    pTPGI (galactose+aTc inducible)

    Available since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. EC2_4_dCas9_VPR_sgRNA

    Plasmid

    #163709

    Purpose

    Yeast low copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    dCas9
    VPR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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