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Plasmid#140148PurposeGAL4-DNA binding domain fusion protein with amino acids 159 to 329 of human KLF4 (repressor domain)DepositorInsertKLF4 aa 159-329 (KLF4 Human)
UseTagsGAL4dbdExpressionMammalianMutationDeleted amino acids 1 to 158 and 330 to 479PromoterEF1aAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
EW269 CMV-TO-Gal4-NZF[GS Linkers] (FLP-IN)
Plasmid#236122PurposePlasmid encoding Gal4-NZF transcription factor with GS linkers between the component domains of NZF, under control of CMV promoter with two TetR binding sitesDepositorInsertGal4-NZF (GS Linkers)
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
plx208-CMV-Gal4DBD-CapN-SsrA-CapC
Plasmid#213533PurposeExpresses one of the split transcription factor component in mammalian cellsDepositorInsertSspB-EGFP-VP16
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
EW152 CMV-TO-Gal4-NZF[Mutated Linkers] (FLP-IN)
Plasmid#236123PurposePlasmid encoding Gal4-NZF transcription factor with deimmunized linkers between the component domains of NZF, under control of CMV promoter with two TetR binding sitesDepositorInsertGal4-NZF (mutated linkers)
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-SspB-EGFP-VP16-P2A-FLAG-Gal4DBD-CapN-SsrA-CapC-HA
Plasmid#213536PurposeExpresses the split transcription factors in mouse liverDepositorInsertSspB-EGFP-VP16-P2A-FLAG-Gal4DBD-CapN-SsrA-CapC-HA-HA
UseAAVTagsEGFP and HAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACT2 V1H (P#663)
Plasmid#14665DepositorInsertV1H (ATP6V1H Human)
UseTagsGal4 ADExpressionYeastMutationPromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
35S-VP16-GWY/pAM
Plasmid#201234PurposeUsed to produce and express in planta C-terminal fusion proteins with the VP16 activation domainDepositorInsert35S-VP16-GWY
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
35S-GWY-VP16/pAM
Plasmid#201232PurposeUsed to produce and express in planta C-terminal fusion proteins with the VP16 activation domainDepositorInsert35S-GWY-VP16
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAS-hActin-luc2
Plasmid#48081PurposeLuciferase Reporter with Gal4 DNA binding site upstream of hACTB promoterDepositorInsertLuciferase
UseTagsExpressionMammalianMutationremoved hsp70 promoter, inserted amplified 615 bp…PromoterAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOAD
Plasmid#196825PurposeYeast two-hybrid ; Gal4 activation domainDepositorTypeEmpty backboneUseTagsGal4 Activation domainExpressionYeastMutationPromoterADHAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOBD2
Plasmid#196826PurposeYeast two-hybrid ; Gal4 DNA binding domainDepositorTypeEmpty backboneUseTagsGal4 DNA binding domainExpressionYeastMutationPromoterADHAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHES941
Plasmid#87942PurposeConstitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Gal4 binding sites.DepositorInsertGAL4 DBD - hPR LBD - MSN2 AD (PGR Human, Budding Yeast, Synthetic)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ths pUAST-HA
Plasmid#69769PurposeThisbe (FGF8-like-1) Ligand in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertThisbe (FGF8like-1)
UseP element-based puast vector for gal4-regulated e…TagsHA-TagExpressionInsectMutationEncodes amino acids 1-739 of Thisbe (NP_610701.2)…Promoterhsp70 promoterAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUAST-HA, Pbl-A
Plasmid#69792PurposePebble isoform A in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertPebble - Drosophila guanine nucleotide exchange factor
UseP element-based puast vector for gal4-regulated e…TagsHA tagExpressionInsectMutationEncodes Pbl (NP_729306.1) lacking last 9 amino ac…Promoterhsp70 promoterAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHES839
Plasmid#87941PurposeConstitutively expresses estradiol-inducible synthetic transcriptional regulator in yeast. Transcriptional regulator activates transcription of promoters containing Gal4 binding sites.DepositorInsertGAL4 DBD - hER LBD - MSN2 AD (ESR1 Human, Budding Yeast, Synthetic)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterADH1 (crippled)Available sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
Pyr pUAST-HA
Plasmid#69768PurposePyramus (FGF8-like-2) Ligand in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertPyramus (FGF8like-2) (pyr Fly)
UseP element-based puast vector for gal4-regulated e…TagsHA-TagExpressionInsectMutation241T amino acid insertion compared to reference s…Promoterhsp70 promoterAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUAST-HA, Pbl-A C-Term
Plasmid#69795PurposePebble isoform A, C-Term, in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertPebble C-Term domain (Drosophila guanine nucleotide exchange factor)
UseP element-based puast vector for gal4-regulated e…TagsHA-TagExpressionInsectMutationJust the C-Term domain of Pebble (pbl)Promoterhsp70 promoterAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBKCg
Plasmid#20162DepositorTypeEmpty backboneUseTagsGal4 DBDExpressionYeastMutationPromoterAvailable sinceMay 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGADCg
Plasmid#20161DepositorTypeEmpty backboneUseTagsGal4 AD and SV40 NLSExpressionYeastMutationPromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -