We narrowed to 1,322 results for: pET28a

1. pET28a-ec_bPAC

   Plasmid

   #28135

   Purpose

   Expression of photoactivated cyclase bPAC of Beggiatoa sp. optimized for E. coli

   Depositor

   Peter Hegemann

   Article

   Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

   Insert

   E.coli adapted photoactivated adenylyl cyclase
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a His6HuR

   Plasmid

   #135943

   Purpose

   Expresses HuR in E. coli

   Depositor

   Yimon Aye

   Article

   Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

   Insert

   ELAV like RNA binding protein 1 (ELAVL1 Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-SUPREM

   Plasmid

   #205023

   Purpose

   Expressing SUPer Rna Ecogii Methyltransferase (SUPREM)

   Depositor

   Paola Laurino

   Article

   Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

   Insert

   SUPer Rna Ecogii Methyltransferase
   

   Use

   Tags

   His6x tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a(+)-SUMO-A'

   Plasmid

   #209115

   Purpose

   Bacterial expression of CC-GEMS ligand SUMO-A'

   Depositor

   Tom de Greef

   Article

   Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

   Insert

   SUMO-A'
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-SpyCatcher

   Plasmid

   #223633

   Purpose

   Protein purification of SpyCatcher003 (S49C) with 6xHis tag from E. coli

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   SpyCatcher003 S49C
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-PTBP1CCtoSS

   Plasmid

   #89157

   Purpose

   Recombinant expression of PTBP1 C250S,C251S in E.coli

   Depositor

   Frédéric Allain

   Article

   Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

   Insert

   Polypyrimidine Tract Binding Protein 1 mutant Cys250Ser, Cys251Ser (PTBP1 Human)
   

   Use

   Tags

   hexa His

   Expression

   Bacterial

   Mutation

   mutations: Cys 250 to Ser, Cys 251 to Ser

   Promoter

   T7

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-HsSENP2

   Plasmid

   #71466

   Purpose

   Encodes human full length SENP2 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

   Depositor

   Jorge Eduardo Azevedo

   Article

   Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

   Insert

   SUMO1/sentrin specific peptidase 2 (SENP2 Human)
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Gene optimised for E. coli expression (Genscript)

   Promoter

   T7

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pET28a-MSP1D1deltaH4H5

   Plasmid

   #71716

   Purpose

   Membrane scaffold protein (MSP), helix 4+ helix 5 deletion

   Depositor

   Gerhard Wagner

   Article

   Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

   Insert

   Apolipoprotein A1 (APOA1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-SET

   Plasmid

   #234708

   Purpose

   expresses His-tagged human SET in E coli

   Depositor

   Lienhard Schmitz

   Article

   Seibert et al J Cell Biol. 2019 Apr 1;218(4):1164-1181

   Insert

   SET (SET Human)
   

   Use

   Tags

   (His)6

   Expression

   Bacterial

   Mutation

   Promoter

   lacI

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Nampt-PET28a

    Plasmid

    #25630

    Depositor

    Cynthia Wolberger

    Article

    Wang et al Nat Struct Mol Biol. 2006 Jul . 13(7):66

    Insert

    Nampt (Nampt Mouse)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 9, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pET28a-cMSP1E3D1

    Plasmid

    #199772

    Purpose

    Expresses split-intein fused MSP1E3D1 for circularization during expression (external His-Tag)

    Depositor

    Franz Hagn

    Article

    Daniilidis et al J Mol Biol. 2022 Dec 30;434(24):167861.

    Insert

    cMSP1E3D1
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    Extended cMSP1D1; external His-Tags flanking the …

    Promoter

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-Ec.coaA

    Plasmid

    #50386

    Purpose

    Heterologous expression of E. coli CoA biosynthetic enzyme, PanK (pantothenate kinase), induced with IPTG. Contain 6xHis-tag for nickel affinity purification.

    Depositor

    Erick Strauss

    Article

    Brand et al J Biol Chem. 2005 May 27;280(21):20185-8

    Insert

    PanK
    

    Use

    Tags

    6XHis-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-HsSENP3

    Plasmid

    #71467

    Purpose

    Encodes human full length SENP3. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 3 (SENP3 Human)
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Gene optimised for E. coli expression (Genscript)

    Promoter

    T7

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-CPDSalI

    Plasmid

    #38252

    Depositor

    Aimee Shen, Matthew Bogyo

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Type

    Empty backbone

    Use

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a(+)::NL3F10H

    Plasmid

    #141290

    Purpose

    NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Insert

    NLUC3F10H
    

    Use

    Luciferase

    Tags

    Flag (x3) and His (x10)

    Expression

    Bacterial

    Mutation

    Engineered for high stability (t1/2 = 11.5 days a…

    Promoter

    promoter for bacteriophage T7 RNA polymerase

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-FIP3

    Plasmid

    #74753

    Purpose

    Expressed sfGFP-tagged FIP3 in E. coli for protein production

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    Rab11 family interacting protein 3 (RAB11FIP3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-TRBP

    Plasmid

    #50351

    Purpose

    bacterial expression of TRBP

    Depositor

    Kumiko Ui-Tei

    Article

    Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

    Insert

    TRBP (TARBP2 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-SpySrtA

    Plasmid

    #51139

    Purpose

    expression plasmid for N-terminal his tagged S.pyogenes SrtA

    Depositor

    Hidde Ploegh

    Article

    Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

    Insert

    S.pyogenes SrtA
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-80

    Promoter

    T7

    Available Since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-PACT

    Plasmid

    #50352

    Purpose

    bacterial expression of PACT

    Depositor

    Kumiko Ui-Tei

    Article

    Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

    Insert

    PACT (PRKRA Human)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-AncMEcoGII291

    Plasmid

    #205024

    Purpose

    Expressing engineered M.EcoGII with higher thermostability and DNA methylation activity

    Depositor

    Paola Laurino

    Article

    Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

    Insert

    Ancestral M.EcoGII 291
    

    Use

    Tags

    His6x tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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