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  13. pTXB1-xlH2B(1-114)_K114A

    Plasmid
    #118217
    Purpose
    Bacterial expression plasmid encoding X. laevis H2B residues 1-114(K114A) as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa.
    Depositor
    Cynthia Wolberger
    Article
    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do
    Insert
    xlH2B(1-114)
    Use
    Tags
    Mxe-GyrA intein fused to chitin binding domain
    Expression
    Bacterial
    Mutation
    Lysine 114 to alanine
    Promoter
    T7
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    118217-plasmid-map-sequence-id-226718

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  18. pEW108

    Plasmid
    #232823
    Purpose
    Expresses yeast compass complex in insect cells
    Depositor
    Cynthia Wolberger
    Article
    Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1
    Insert
    COMPASS (Cps60, Cps50, Cps35, Cps25, Cps15, Flag-Set1, Cps30, Twinstrep-Cps40) (BRE2, SWD1, SET1, SPP1, SWD3, SWD2, SDC1, SHG1 Budding Yeast)
    Use
    Tags
    His6-3xFLAG, Twinstrep
    Expression
    Insect
    Mutation
    Set1(762-1080)
    Promoter
    polyhedrin promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232823-plasmid-map-sequence-id-465728

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  19. pEW107

    Plasmid
    #232796
    Purpose
    COMPASS Fragment 2 (His-FLAG-ySet1, Cps30, Twinstrep-Cps40) in pBIG2ab
    Depositor
    Cynthia Wolberger
    Article
    Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1
    Insert
    COMPASS Fragment 2 (His-FLAG-ySet1, Cps30, Twinstrep-Cps40) (SET1, SPP1, SWD3 Budding Yeast)
    Use
    Tags
    His6-3xFLAG, Twinstrep
    Expression
    Insect
    Mutation
    ySet1(762-1080)
    Promoter
    polyhedrin promoter
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    232796-plasmid-map-sequence-id-465733

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Showing: 21 - 40 of 44 results