372 results

1. NES-EGFP-PH-BTKx2

   Plasmid

   #183652

   Purpose

   PtdIns(3,4,5)P3 biosensor

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   BTK (BTK Human, Frog)
   

   Use

   Tags

   X. laevis map2k1.L(32-44)-EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 2-174 and 2-170

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Xenopus C-cadherin

   Plasmid

   #17023

   Depositor

   Barry Gumbiner

   Article

   Lee et al Dev Biol. 1995 Oct . 171(2):363-73.

   Insert

   C-cadherin (cdh3.S Frog)
   

   Use

   Xenopus expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pCS2 Geminin

   Plasmid

   #12174

   Depositor

   Marc Kirschner

   Article

   McGarry et al Cell. 1998 Jun 12. 93(6):1043-53.

   Insert

   Geminin (gmnn Frog)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 7, 2006

   Availability

   Academic Institutions and Nonprofits only

4. GSK3beta/pET29b

   Plasmid

   #16315

   Depositor

   Peter Klein

   Article

   Hedgepeth et al Dev Biol. 1997 May 1. 185(1):82-91.

   Insert

   GSK3beta (gsk3b.L Frog)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

5. M12 pCarGFP2

   Plasmid

   #17149

   Depositor

   Randall Moon

   Insert

   cardiac actin promoter
   

   Use

   Xenopus expression

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

6. NES-mCherry-PH-BTKx2

   Plasmid

   #183654

   Purpose

   PtdIns(3,4,5)P3 biosensor

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   BTK (BTK Human, Frog)
   

   Use

   Tags

   X. laevis map2k1.L(32-44)-mCherry

   Expression

   Mammalian

   Mutation

   Amino acids 2-174 and 2-170

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CDK1as_T2A_Zeo

   Plasmid

   #118596

   Purpose

   One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.

   Depositor

   William Earnshaw

   Article

   Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

   Insert

   CDK1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. XE246 Xenopus CK1 epsilon D to N CS2+

   Plasmid

   #16726

   Depositor

   Randall Moon

   Article

   Waxman et al Development. 2004 Dec. 131(23):5909-21.

   Insert

   casein kinase 1, epsilon (csnk1a1 Frog)
   

   Use

   Xenopus expression

   Tags

   Expression

   Mutation

   D128N

   Promoter

   Available since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

9. XE10 Xwnt-8 CS2+

   Plasmid

   #16865

   Depositor

   Randall Moon

   Insert

   Xwnt-8 (wnt8a.L Frog)
   

   Use

   Xenopus expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 13, 2008

   Availability

   Academic Institutions and Nonprofits only

10. C245

    Plasmid

    #174351

    Purpose

    Template for making mRNA for overexpressing frog RPL3 (C-HA) in frog oocytes

    Depositor

    David Bartel

    Article

    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

    Insert

    rpl3.L (rpl3.L Frog)
    

    Use

    In vitro transcription template

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. hE-cadherin/α-catenin fusion-pcDNA3

    Plasmid

    #45771

    Depositor

    Barry Gumbiner

    Article

    Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

    Insert

    hE-cadherin/Xe α-catenin fusion (CDH1, ctnna1 Human, Frog)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Human E-cadherin (aa 1-801) fused to Xenopus α-ca…

    Promoter

    CMV

    Available since

    June 14, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pKE12-ISceI-fabp10a:loxP-BFP-loxP-Xla.Ctnnb1

    Plasmid

    #198240

    Purpose

    For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter drives expression of activated β-catenin preceded by a blue fluorescent protein (BFP)-STOP cassette flanked by loxP sites

    Depositor

    Didier Stainier

    Article

    Kalasekar et al Biol Open. 2019 Oct 17;8(10):bio047829.

    Inserts

    Zebrafish fabp10a promoter:floxedBFP, Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
    Zebrafish cryaa promoter:mcherry fluorescent protein coding sequence (cryaa Zebrafish)
    

    Use

    Zebrafish transgenesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTol2_NBT:Cas9green; erbb2_gRNA171

    Plasmid

    #199337

    Purpose

    pDEL134; transgenic construct to express cell-specific Cas9 in neurons; ubiquitous erbb2 sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    NBT
    Cas9
    erbb2 sgRNA
    

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CaM/pIRES2-eGFP

    Plasmid

    #111499

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    calmodulin [Xenopus laevis] (calm2 Frog)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. H2BK120C

    Plasmid

    #75332

    Purpose

    Bacteria expression of H2BK120C

    Depositor

    Cynthia Wolberger

    Article

    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

    Insert

    H2BK120C (h2bc11.S Frog)
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    truncated; K120C

    Promoter

    T7

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCS2xVlk1-3xFlag

    Plasmid

    #172773

    Purpose

    mRNA synthesis for injection into embryos

    Depositor

    Edward De Robertis

    Article

    Ding et al Dev Biol. 2017 Jun 15;426(2):176-187. do

    Insert

    Vlk1 (pkdcc.1.S Frog)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. KSP_K560GFP

    Plasmid

    #129770

    Purpose

    Expresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged

    Depositor

    William Hancock

    Insert

    KSP (kif11.L Frog)
    

    Use

    Tags

    GFP and His6

    Expression

    Bacterial

    Mutation

    Truncation at 367, fused to kinesin-1 starting Al…

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCS2 xenopus sizzled

    Plasmid

    #24684

    Depositor

    Edward De Robertis

    Article

    Lee et al Cell. 2006 Jan 13. 124(1):147-59.

    Insert

    Sizzled (szl Frog)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pCS2-XTbx5-GR

    Plasmid

    #117248

    Purpose

    mRNA synthesis

    Depositor

    Gerald Thomsen

    Article

    Horb et al Development. 1999 Apr;126(8):1739-51.

    Insert

    Tbx5-GR (tbx5-b Frog)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    chimeric protein with human glucocorticoid recep…

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCS2 DN-GSK3-GFP

    Plasmid

    #29681

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    glycogen synthase kinase 3
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K85R and K86R

    Promoter

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only