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lentiCRISPRv2-puro-dHbegf

Plasmid

#173841

Purpose

A knockout vector for the dog Hbegf.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Hbegf gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-neo-dTgfa

Plasmid

#173842

Purpose

A knockout vector for the dog Tgfa.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Tgfa gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-hyg2-dEreg

Plasmid

#173843

Purpose

A knockout vector for the dog Ereg.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Ereg gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-puro-dEgfr

Plasmid

#173844

Purpose

A knockout vector for the dog Egfr.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Egfr gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pZA44

Plasmid

#158522

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V/L17E-eGFP. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-eGFP (ZAR1 Mustard Weed)

Tags

eGFP

Expression

Plant

Mutation

L17E/D489V

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pZA16

Plasmid

#158494

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V/L17E without STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Use

Golden gate entry vector

Mutation

L17E, D481V, no STOP codon

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pZA7

Plasmid

#158485

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Use

Golden gate entry vector

Mutation

L17E, STOP codon

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pZA15

Plasmid

#158493

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E without STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Use

Golden gate entry vector

Mutation

L17E, no STOP codon

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pZA18

Plasmid

#158496

Purpose

In planta gene expression of Golden gate compatibleA. thaliana ZAR1 D489V. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Expression

Plant

Mutation

D489V

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pZA36

Plasmid

#158514

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V/L17E-6xHA. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-6xHA (ZAR1 Mustard Weed)

Tags

6xHA

Expression

Plant

Mutation

L17E, D489V

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pZA6

Plasmid

#158484

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Use

Golden gate entry vector

Mutation

D481V, STOP codon

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA14

Plasmid

#158492

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V without STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Use

Golden gate entry vector

Mutation

D481V, no STOP codon

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA19

Plasmid

#158497

Purpose

In planta gene expression of Golden gate compatlible A. thaliana ZAR1 L17E. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Expression

Plant

Mutation

L17E

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pZA35

Plasmid

#158513

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1 L17E-6xHA. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-6xHA (ZAR1 Mustard Weed)

Tags

6xHA

Expression

Plant

Mutation

L17E

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pZA33

Plasmid

#158511

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1-6xHA. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-6xHA (ZAR1 Mustard Weed)

Tags

6xHA

Expression

Plant

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA20

Plasmid

#158498

Purpose

In planta gene expression of Golden gate complatible A. thaliana ZAR1 D498V/L17E. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Expression

Plant

Mutation

L17E, D489V

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA41

Plasmid

#158519

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1-eGFP. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-eGFP (ZAR1 Mustard Weed)

Tags

eGFP

Expression

Plant

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA43

Plasmid

#158521

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1 L17E-eGFP. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-eGFP (ZAR1 Mustard Weed)

Tags

eGFP

Expression

Plant

Mutation

L17E

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA42

Plasmid

#158520

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V-eGFP. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-eGFP (ZAR1 Mustard Weed)

Tags

eGFP

Expression

Plant

Mutation

D489V

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA34

Plasmid

#158512

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V-6xHA. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-6xHA (ZAR1 Mustard Weed)

Tags

6xHA

Expression

Plant

Mutation

D489V

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA28

Plasmid

#158506

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V/L17E-4xMyc. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-4xMyc (ZAR1 Mustard Weed)

Tags

4xMyc

Expression

Plant

Mutation

L17E, D489V

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA25

Plasmid

#158503

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1-4xMyc. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-4xMyc (ZAR1 Mustard Weed)

Tags

4xMyc

Expression

Plant

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-B2 (GB3374)

Plasmid

#160636

Purpose

pLX series: pBBR1-based T-DNA binary vector for plant transformation (alpha1)

Depositor

Juan Antonio García, Fabio Pasin

Article

Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

Type

Empty backbone

Use

Synthetic Biology

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-P3-IgKL-SpyCatcher003-mNeonGreen

Plasmid

#234543

Purpose

to express a green fluorescent protein tagged with SpyCatcher in the liver

Depositor

Hajime Hirase

Article

Vittani et al bioRxiv 2025.03.18.643859

Insert

IgKL-SpyCatcher003-mNeonGreen

Use

AAV

Mutation

None

Promoter

P3

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-P3-IgKL-SpyCatcher003-SEP

Plasmid

#234541

Purpose

to express a pH sensor tagged with SpyCatcher in the liver

Depositor

Hajime Hirase

Article

Vittani et al bioRxiv 2025.03.18.643859

Insert

IgKL-SpyCatcher003-SEP

Use

AAV

Mutation

None

Promoter

P3

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pNG1256

Plasmid

#149710

Purpose

Expresses RpoA, RpoB, RpoC-9His and RpoZ from Bacillus subtilis to produce core RNAP complex

Depositor

Peter Lewis

Article

Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

Insert

rpoZ (rpoZ )

Expression

Bacterial

Promoter

T7

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-SARS-CoV-2-Nsp3PLpro

Plasmid

#204208

Purpose

Mammalian expression of SARS-CoV-2 Nsp3

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )

Tags

FLAGx1

Expression

Mammalian

Mutation

PLpro of Nsp3

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PMXS-SLC1A3

Plasmid

#72873

Purpose

The retroviral SLC1A3 vector was generated by cloning an human SLC1A3 PCR fragment (https://www.addgene.org/32813/)into the pMXS-ires-blast vector by Gibson Assembly

Depositor

David Sabatini

Article

Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

Insert

SLC1A3 (SLC1A3 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pLX-Z4 (GB3375)

Plasmid

#160648

Purpose

pLX series: RK2-based T-DNA binary vector for plant transformation (alpha1)

Depositor

Juan Antonio García, Fabio Pasin

Article

Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

Type

Empty backbone

Use

Synthetic Biology

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-B3omega1 (GB1694)

Plasmid

#160643

Purpose

pLX series: pBBR1-based T-DNA binary vector for plant transformation (omega1)

Depositor

Juan Antonio García, Fabio Pasin

Article

Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

Type

Empty backbone

Use

Synthetic Biology

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSG4K5

Plasmid

#74492

Purpose

For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.

Depositor

Xiao Wang

Article

Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2

Insert

sgRNA

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFBP-2_6.sensor

Plasmid

#162800

Purpose

Yeast expression of the RNA-based sensor for fructose-1,6-bisphosphate #2_6

Depositor

Matthias Heinemann

Article

Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

Insert

2_6_RNA-device

Expression

Yeast

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV5.1-SARS-CoV-2 Nsp3

Plasmid

#204205

Purpose

Mammalian expression of SARS-CoV-2 Nsp3

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )

Tags

FLAGx3

Expression

Mammalian

Mutation

none, from obtained clone

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pG2J

Plasmid

#102882

Purpose

Expresses OsTIR1 via the ADH1 promoter, and mEGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.

Depositor

Matthias Heinemann

Article

Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

Inserts

OsTIR1
mEGFP-AID

Tags

3xMyc and truncated AID (71-114)

Expression

Yeast

Promoter

ADH1 and TEF1

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPRv2puro-Ptgs2_B6

Plasmid

#170300

Purpose

A knock-out vector for the mouse Ptgs2 in C57BL6

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A gRNA targeting the mouse tyrosinase gene.

Use

CRISPR and Lentiviral

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPRv2hyg-Ptgs1

Plasmid

#170299

Purpose

A knock-out vector for the mouse Ptgs1

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A gRNA targeting the mouse Ptgs1 gene.

Use

CRISPR and Lentiviral

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-SARS-CoV-2 Nsp3TM

Plasmid

#204206

Purpose

Mammalian expression of SARS-CoV-2 Nsp3

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )

Tags

FLAGx1

Expression

Mammalian

Mutation

none, from obtained clone

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPRv2puro-Tbxas1

Plasmid

#170305

Purpose

A knock-out vector for the mouse Tbxas1

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A gRNA targeting the mouse Tbxas1 gene.

Use

CRISPR and Lentiviral

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPRv2puro-Tbxa2r

Plasmid

#170304

Purpose

A knock-out vector for the mouse Tbxa2r

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A gRNA targeting the mouse Tbxa2r gene.

Use

CRISPR and Lentiviral

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFBP-4_1.sensor

Plasmid

#162844

Purpose

Yeast expression of the RNA-based sensor for fructose-1,6-bisphosphate #4_1

Depositor

Matthias Heinemann

Article

Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

Insert

4_1_RNA-device

Expression

Yeast

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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