Addgene

We narrowed to 2,096 results for: AP1

Showing: 951 - 1000 of 2096 results

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p8180 pHAGE N-V5 PTPN14 ΔPPXY3/4

Plasmid

#221647

Purpose

Constitutive ectopic expression of human PTPN14 ΔPPXY3/4 mutant

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

PTPN14 ΔPPXY3/4 (PTPN14 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

deletion of the third and fourth PPXY motifs as d…

Promoter

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
p8189 pHAGE N-V5 PTPN14 C1121S

Plasmid

#221648

Purpose

Constitutive ectopic expression of human PTPN14 C1121S mutant

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

PTPN14 C1121S (PTPN14 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

C1121S mutation at the putative catalytic site

Promoter

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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p8391 LentiCRISPRv2 Neo sgPTPN14-1

Plasmid

#221651

Purpose

Expression of sgRNA targeting the locus of human PTPN14

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

sgPTPN14-1 (PTPN14 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
p8392 LentiCRISPRv2 Neo sgPTPN14-3

Plasmid

#221652

Purpose

Expression of sgRNA targeting the locus of human PTPN14

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

sgPTPN14-3 (PTPN14 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1-IFT22

Plasmid

#218720

Purpose

Expresses N-terminally EGFP-tagged IFT22 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

IFT22 (RABL5 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac-Dual-GST-TEV-SINTBAD-mCherry

Plasmid

#208874

Purpose

For expression in insect cells of SINTBAD-mCherry

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

SINTBAD (TBKBP1 Human)

Use

Tags

GST-TEV

Expression

Insect

Mutation

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
p3xFLAG-p62 var2 PB1mut

Plasmid

#204578

Purpose

Expresses 3xFLAG-p62 var2 PB1mut in mammalian cells

Depositor

Masaaki Komatsu

Article

Kageyama et al Mol Cell Biol. 2018 Jan 16. pii: MCB.006

Insert

p62 (Sqstm1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

K7A/D69A

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV puro PRDM14

Plasmid

#193677

Purpose

Constitutive retroviral expression of PRDM14

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

PRDM14 (PRDM14 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX304 PRDM14

Plasmid

#193688

Purpose

Constitutive lentiviral expression of PRDM14

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

PRDM14 (PRDM14 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-RanGAP

Plasmid

#175235

Purpose

Bacterial expression and purification, high affinity SUMOylation substrate

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

RANGAP1 (RANGAP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBG-TFII-I L30&37P

Plasmid

#22166

Depositor

Ananda Roy

Article

Sacristan et al J Biol Chem. 2004 Feb 20. 279(8):7147-58

Insert

TFII-I (GTF2I Human)

Use

Tags

GST and His

Expression

Mammalian

Mutation

Changed Leucine 30 to Proline and changed leucine…

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-MTOR-S2215Y

Plasmid

#69013

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR (MTOR Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S2215Y

Promoter

CMV

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PHB1-mCherry

Plasmid

#232784

Purpose

Encodes an mCherry-tagged Prohibitin1/PHB1

Depositor

Giulia Bertolin

Article

Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

Insert

PHB1 (PHB1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
PHB2-mCherry

Plasmid

#232785

Purpose

Encodes an mCherry-tagged Prohibitin2/PHB2

Depositor

Giulia Bertolin

Article

Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

Insert

PHB2 (PHB2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
PHB2-GFP

Plasmid

#232788

Purpose

Encodes a GFP-tagged Prohibitin2/PHB2

Depositor

Giulia Bertolin

Article

Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

Insert

PHB2 (PHB2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
TBX5-FLAG in pLX303

Plasmid

#42563

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

TBX5 (TBX5 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
shTBX5 # 2

Plasmid

#42550

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shTBX5 (TBX5 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
shTBX5 # 1

Plasmid

#42549

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shTBX5 (TBX5 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA3-Myc3-Nrf2

Plasmid

#21555

Purpose

Mammalian expression of human Nrf2 with Myc tag

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

Insert

nuclear factor (erythroid-derived 2)-like 2 (NFE2L2 Human)

Use

Tags

myc3

Expression

Mammalian

Mutation

Promoter

Available since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
AP-1 mu1-GFP

Plasmid

#173432

Purpose

Expression of AP-1 Mu1 subunit with a terminal GFP

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP1M1 (Ap1m1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAcGFP-Hyg-CTCF-N1

Plasmid

#131799

Purpose

Expresses human CTCF-GFP in mammalian cells through transient transfection

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

CTCF (CTCF Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28HIS-hAPE1

Plasmid

#70757

Purpose

bacterial expression of human APE1

Depositor

Primo Schaer

Article

Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

Insert

APEX1 (APEX1 Human)

Use

Tags

6-HIS

Expression

Bacterial

Mutation

Promoter

T7/lac operon

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
myc-KIF5C

Plasmid

#127618

Purpose

N-terminal myc-tagged mouse KIF5C

Depositor

Josef Kittler

Article

Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

Insert

KIF5C (Kif5c Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 HA Arf6 DN T27N

Plasmid

#10831

Depositor

Thomas Roberts

Article

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf6 T27N (ARF6 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

T27N. Defective in GTP binding. Dominant negati…

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 HA Arf1 ActQ71L

Plasmid

#10832

Depositor

Thomas Roberts

Article

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf1 Q71L (ARF1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Q71L. Defective in GTP hydrolysis. Constitutive…

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 HA Arf1 DN T31N

Plasmid

#10833

Depositor

Thomas Roberts

Article

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf1 T31N (ARF1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

T31N. Defective in GTP binding. Dominant negati…

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pmiRGLO Dual-Luciferase-HNRNPAB-3'UTR

Plasmid

#182249

Purpose

Human HNRNPAB 3` UTR region containing 2x binding sites for miR-150 cloned downstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

Insert

3' UTR region of HNRNPAB gene

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pmiRGLO Dual-Luciferase-MYB-3'UTR

Plasmid

#182250

Purpose

Human MYB 3` UTR region containing 3x binding sites for miR-150 cloned downstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

Insert

3' UTR region of MYB gene

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKH3-TRIM28

Plasmid

#45569

Depositor

Fanxiu Zhu

Article

Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

Insert

TRIM28 (TRIM28 Human)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 29, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
1432 pcDNA3 flag MAGI1c

Plasmid

#10714

Depositor

William Sellers

Article

Vazquez et al J Biol Chem. 2001 Dec 28. 276(52):48627-

Insert

MAGI-1 (Magi1 Mouse)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 28, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-TRIM28

Plasmid

#45568

Depositor

Fanxiu Zhu

Article

Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

Insert

TRIM28 (TRIM28 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

G34S mutation in EGFP

Promoter

CMV

Available since

May 29, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGEX-5X-TRIM28

Plasmid

#45570

Depositor

Fanxiu Zhu

Article

Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

Insert

TRIM28 (TRIM28 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Ptac

Available since

May 29, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pMSCV mSin1.2 myc

Plasmid

#12577

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Aug 15. ():.

Insert

mSin1.2 (MAPKAP1 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pMSCV mSin1.5 myc

Plasmid

#12579

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Aug 15. ():.

Insert

mSin1.5 (MAPKAP1 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pMSCV mSin1.4 myc

Plasmid

#12578

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Aug 15. ():.

Insert

mSin1.4 (MAPKAP1 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3 HA Arf6 ActQ67L

Plasmid

#10835

Depositor

Thomas Roberts

Article

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf6 Q67L (ARF6 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Q67L. Defective in GTP hydrolysis. Constitutive…

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG-MTOR-V2006I

Plasmid

#69012

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR (MTOR Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

V2006I

Promoter

CMV

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX317 SLC2A1

Plasmid

#193685

Purpose

Constitutive lentiviral expression of SLC2A1

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

SLC2A1 (SLC2A1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
sgTrack-BFP

Plasmid

#164273

Purpose

Empty sgRNA expression vector with co-expression of BFP reporter

Depositor

Charles Rudin

Article

Wohlhieter et al Cell Rep. 2020 Dec 1;33(9):108444. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 and EFS

Available since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
myc-KIF5B

Plasmid

#127617

Purpose

N-terminal myc-tagged mouse KIF5B

Depositor

Josef Kittler

Article

Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

Insert

KIF5B (Kif5b Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1-Cdc42BPB

Plasmid

#50759

Purpose

expression of EGFP tagged Cdc42BPB

Depositor

Naoki Mochizuki

Article

Ando et al J Cell Biol. 2013 Sep 16;202(6):901-16.

Insert

Cdc42BPB (CDC42BPB Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
shTCF7L2 # 1

Plasmid

#42557

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shTCF7L2 (TCF7L2 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only
pmiRGLO Dual-Luciferase-PIK3R1-3'UTR

Plasmid

#182252

Purpose

Human PIK3R1 3` UTR region containing 1x binding site for miR-150 cloned downstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

Insert

3' UTR region of PIK3R1 gene

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV mSin1.1 myc

Plasmid

#12576

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Aug 15. ():.

Insert

mSin1.1 (MAPKAP1 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pLL5.0 hCortKD EGFP

Plasmid

#187267

Purpose

Lentiviral expression of shRNA targeting Cortactin

Depositor

Alpha Yap

Article

Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

Insert

shRNA for Cortactin (CTTN Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

U6

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1-IFT54

Plasmid

#218726

Purpose

Expresses N-terminally EGFP-tagged IFT54 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Hiyamizu et al J Cell Sci. 2023 Mar 1;136(5):jcs260462.

Insert

IFT54 (TRAF3IP1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX304 SLC2A1

Plasmid

#193691

Purpose

Constitutive lentiviral expression of SLC2A1

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

SLC2A1 (SLC2A1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
ELK4_pet28a

Plasmid

#131648

Purpose

Bacterial expression of ETS gene ELK4

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELK4 (ELK4 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
shTCF7L2 # 2

Plasmid

#42558

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shTCF7L2 (TCF7L2 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3flag-RanGAPdeltaN

Plasmid

#25104

Depositor

Guy Salvesen

Article

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

RanGAP1 deleted N-term domain (RANGAP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Deleted amino acids 1-203.

Promoter

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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