We narrowed to 9,586 results for: Coli
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Plasmid#185049PurposeJ23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region H6A mutation, trrnBDepositorInsertL31p
ExpressionBacterialMutationH6A amino acid mutaitonsAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR114
Plasmid#185050PurposeJ23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region E8A mutation, trrnBDepositorInsertL31p
ExpressionBacterialMutationE8A amino acid mutationAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR089-L36p
Plasmid#185042PurposeJ23108(spel), pHP14 stability hairpin, strong RBS, L36p coding region, trrnBDepositorInsertL36p
ExpressionBacterialAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR090-L36
Plasmid#185043PurposeJ23108(spel), pHP14 stability hairpin, strong RBS, L36 coding region, trrnBDepositorInsertL36
ExpressionBacterialAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR091-L31p
Plasmid#185044PurposeJ23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region, trrnBDepositorInsertL31p
ExpressionBacterialAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7-10xHis-ecRNHI*
Plasmid#219523Purposebacterial expression of cysteine-free variant of the E. coli ribonuclease HI geneDepositorInsertcysteine-free variant of the E. coli ribonuclease HI gene
TagsN terminal 10xHis TagExpressionBacterialMutationCysteine free variantAvailable SinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBN095
Plasmid#173765Purposeexpress E. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncationDepositorInsertE. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncation
Tagshexa-histidine tagExpressionBacterialMutationdelete motif 3PromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN215
Plasmid#167962Purposeexpress E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718EDepositorInsertE. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E
Tagshexa-histidine tagExpressionBacterialMutationY715E, Y717E, Y718EPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN216
Plasmid#167963Purposeexpress E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718EDepositorInsertE. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E
Tagshexa-histidine tagExpressionBacterialMutationY713E, Y715E, Y717E, Y718EPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN217
Plasmid#167964Purposeexpress E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718EDepositorInsertE. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E
Tagshexa-histidine tagExpressionBacterialMutationY708E, Y713E, Y715E, Y717E, Y718EPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN261
Plasmid#167966Purposeexpress E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717EDepositorInsertE. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E
Tagshexa-histidine tagExpressionBacterialMutationY713E, Y715E, Y717EPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN262
Plasmid#167967Purposeexpress E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717EDepositorInsertE. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E
Tagshexa-histidine tagExpressionBacterialMutationY708E, Y713E, Y715E, Y717EPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBRC901
Plasmid#167948Purposeexpress wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)DepositorInsertwild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)
Tagshexa-histidine tagExpressionBacterialPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN008
Plasmid#167949Purposeexpress E. coli E69 (O9a:K30) Wzc with K540M mutationDepositorInsertbacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30), K540M nutant
Tagshexa-histidine tagExpressionBacterialMutationK540MPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN202
Plasmid#167950Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutationsDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations
Tagshexa-histidine tagExpressionBacterialMutationK540M, Y713E, Y715E, Y717E, Y718EPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN093
Plasmid#167951Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncationDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation
Tagshexa-histidine tagExpressionBacterialMutationK540M, delete motif 3PromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
AlkB-D135G
Plasmid#160434PurposeA plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to GlyDepositorInsertE. coli AlkB
TagsHis-tagExpressionBacterialMutationChanged Aspartic acid 135 to Glycine; deleted the…PromoterT7Available SinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVR
Plasmid#84718PurposeE. coli RecA promoter followed by preQ1 riboswitch from the 5' UTR of the queC gene from B. subtilisDepositorInsertsE. coli RecA promoter
5' UTR of queC gene
ExpressionBacterialAvailable SinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCML 314
Plasmid#98584PurposepCsrA-NYFP + CsrB-2MS2BD. Expresses protein-NYFP and RNA-2MS2BD fusions in E. coli for TriFC assay (ampR).DepositorInsertsCsrA
csrB
ExpressionBacterialPromoterpLacOAvailable SinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMART-Ala1
Plasmid#65814PurposeNADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli ExpressionDepositorInsertNADPH Alanine Dehydrogenase (ald Synthetic)
ExpressionBacterialMutationD196A, L197RPromoterInsulated waaHp from E. coliAvailable SinceJuly 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLRD01
Plasmid#208257PurposeProduction of terpentetriene in E. coli MEV20DepositorInsertstHMGR
cyc1
cyc2
ExpressionBacterialAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad-HPII
Plasmid#105839Purposeexpresses E. coli HPII catalase with N-terminal 6x his tagDepositorArticleInserthydroperoxidase II catalase
Tags6x his tagExpressionBacterialPromoterpBad/arabinoseAvailable SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAT5
Plasmid#112581PurposeUsed for expression of protein in E. coli as non-fusions without initiation ATGDepositorTypeEmpty backboneExpressionBacterialAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLRD26
Plasmid#208273PurposeProduction of levopimaradiene in E. coli MEV20DepositorInsertstHMGR
LPS
ExpressionBacterialAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHL 233
Plasmid#37754DepositorInsertsptet_ompC_gfp
pLac_st7 TetR_mCherry
UseSynthetic Biology; E. coliTagsgfp and mCherryPromoterpLac and ptetAvailable SinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLRD25
Plasmid#208272PurposeProduction of cis-abienol in E. coli MEV20DepositorInsertstHMGR
abCAS
ExpressionBacterialAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a_thyA_R166Q_untagged
Plasmid#191307PurposeBacterial expression of E. coli thyA (thymidylate synthase)DepositorInsertthyA
ExpressionBacterialMutationR166Q TYMSPromoterT7Available SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a_thyA_Q33S_untagged
Plasmid#191308PurposeBacterial expression of E. coli thyA (thymidylate synthase)DepositorInsertthyA
ExpressionBacterialMutationQ33S TYMSPromoterT7Available SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a_thyA_R127A_untagged
Plasmid#191309PurposeBacterial expression of E. coli thyA (thymidylate synthase)DepositorInsertthyA
ExpressionBacterialMutationR127A TYMSPromoterT7Available SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a_thyA_WT_untagged
Plasmid#191306PurposeBacterial expression of E. coli thyA (thymidylate synthase)DepositorInsertthyA
ExpressionBacterialMutationWT TYMSPromoterT7Available SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-fabI-gltA1-gltA2-udhA-zwf
Plasmid#87148PurposefabI-gltA1-gltA2-udhA-zwf targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertfabI-gltA1-gltA2-udhA-zwf guide array
ExpressionBacterialPromoterE . coli ugpB gene promoter, low phosphate induct…Available SinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-fabI-gltA1-gltA2-udhA-gapAP1
Plasmid#87154PurposefabI-gltA1-gltA2-udhA-gapAP1 targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertfabI-gltA1-gltA2-udhA-GapAP1 guide array
ExpressionBacterialPromoterE . coli ugpB gene promoter, low phosphate induct…Available SinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHL 363
Plasmid#37758DepositorInsertspLar_rpoS_gfp
pLtet_dsrA_pLac_araC
UseSynthetic Biology; E. coliTagsgfp and nonePromoterPtet and pLac and pLarAvailable SinceAug. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHL 279
Plasmid#37757DepositorInsertsptet_ompC_gfp
pLac_st3 TetR_mCherry
UseSynthetic Biology; E. coliTagsgfp and mCherryPromoterpLac and ptetAvailable SinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKP1392
Plasmid#167649PurposeExpress ExbD T42C proteinDepositorInsertexbD T42C
ExpressionBacterialMutationexbD with T42C mutationAvailable SinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDest-544
Plasmid#11519DepositorTypeEmpty backboneUseGateway destination vectorTagsHis6-NusAExpressionBacterialAvailable SinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGLU_67
Plasmid#225770PurposeThis is the native promoter from the aph(3')-I kanamycin resistance cassette.DepositorInsertPaph(3')-I_[abr]
UseSynthetic BiologyMutationN/AAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTJK368
Plasmid#138006PurposeBirA* expression. Vector also expresses GFP under control of hUBC promoterDepositorInsertBirA*
UseLentiviralMutationNoneAvailable SinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSG 25
Plasmid#63867PurposeExpression of beta-galactosidase at high levels in E. coliDepositorAvailable SinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRAD1
Plasmid#47697PurposeGeneral cloning vector for use in E. coli or D. radioduransDepositorTypeEmpty backboneExpressionBacterialPromoterrepUAvailable SinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHAT2
Plasmid#112583PurposeUsed for expression of protein in E. coli as non-cleavable minimal N-terminal 6His-tag fusionsDepositorTypeEmpty backboneTags6HisExpressionBacterialAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM6
Plasmid#49795PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneTagsS-tagExpressionBacterialPromoterT7Available SinceJan. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRADZ1
Plasmid#47698PurposeGeneral cloning vector for use in E. coli or D. radiodurans with lacZDepositorTypeEmpty backboneExpressionBacterialPromoterrepUAvailable SinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pACM4
Plasmid#49797PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneTagsS-tagExpressionBacterialPromoterT7Available SinceJan. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDM4
Plasmid#49796PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneTagsS-tagExpressionBacterialPromoterT7Available SinceJan. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSM3
Plasmid#49799PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneTagsS-tagExpressionBacterialPromoterT7Available SinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLRD11
Plasmid#208263PurposeProduction of sclareol in E. coli MEV20DepositorInsertstHMGR
abCAS
ssSS
ExpressionBacterialMutationabCAS(D537A)Available SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ec dnaN-pHK/P
Plasmid#237239Purposeover-express E. coli hk/P-dnaN (Pol III beta sliding clamp subunit, N-terminal cleveable his-kinase tag)DepositorInsertdnaN (beta), N-terminal his-kinase cleavable tag (dnaN E. coli)
Tagshis-10+PKA kinase motif+PreScission Protease clea…ExpressionBacterialAvailable SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLRD14
Plasmid#208266PurposeProduction of syn-pimara-7,15-diene in E. coli MEV20DepositorInsertstHMGR
osCPS4
osKSL4
ExpressionBacterialAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLRD18
Plasmid#208270PurposeProduction of ent-isokaurene in E. coli MEV20DepositorInsertsptmT2
osKSL6
ExpressionBacterialAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only