We narrowed to 22,187 results for: ESC

1. pcDNA-RCC

   Plasmid

   #190635

   Purpose

   Mammalian expression of genetically encoded calcium indicator RCC

   Depositor

   Robert Campbell

   Article

   Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

   Insert

   RCC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA5/FRT/TO_H2B-STf

   Plasmid

   #181998

   Purpose

   CMV overexpression of SNAP-tag fast fusion to H2B for nuclear localization in mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   H2B-STf
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUC19-sg-1

   Plasmid

   #190607

   Purpose

   Expresses a gRNA for base editing the EGFP Kozak sequence of pWPT-/mEGFP-1T-IRES-mCherry

   Depositor

   Alessandro Quattrone

   Article

   Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

   Insert

   gRNA sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUC19-sgNCF1

   Plasmid

   #190193

   Purpose

   Expresses a gRNA for base editing the Kozak sequence of the human NCF1 gene

   Depositor

   Alessandro Quattrone

   Article

   Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

   Insert

   gRNA sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDNA-SCcpV

   Plasmid

   #190636

   Purpose

   Mammalian expression of genetically encoded calcium indicator SCcpV

   Depositor

   Robert Campbell

   Article

   Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

   Insert

   SCcpV
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNCS-Clover4-LUMABS-HIV

   Plasmid

   #183485

   Purpose

   To express the Clover4-LUMABS-HIV sensor for Acquired Immunodeficiency Virus antibody dection.

   Depositor

   Zongwen Jin, Jun Chu

   Article

   Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

   Insert

   Clover4-LUMABS-HIV
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFastBac1-Clover4-LUMABS-S1 RBD

   Plasmid

   #183491

   Purpose

   To express the Clover4-LUMABS-S1 RBD sensor for SARS-CoV-2 antibody dection.

   Depositor

   Zongwen Jin, Jun Chu

   Article

   Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

   Insert

   Clover4-LUMABS-S1 RBD
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNCS-Clover4-LUMABS-EBNA1

   Plasmid

   #183488

   Purpose

   To express the Clover4-LUMABS-EBNA1 sensor for Epstein-barr Virus antibody dection.

   Depositor

   Zongwen Jin, Jun Chu

   Article

   Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

   Insert

   Clover4-LUMABS-EBNA1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNCS-Clover4-LUMABS-VCA

   Plasmid

   #183489

   Purpose

   To express the Clover4-LUMABS-VCA sensor for Epstein-barr Virus antibody dection.

   Depositor

   Zongwen Jin, Jun Chu

   Article

   Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

   Insert

   Clover4-LUMABS-VCA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCML 313

    Plasmid

    #98586

    Purpose

    pNYFP + CsrB-2MS2BD. Expresses NYFP control fusion and RNA-2MS2BD fusion in E. coli for TriFC assay (ampR).

    Depositor

    Lydia Contreras

    Article

    Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

    Insert

    csrB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLacO

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRSETa-Amrose v2

    Plasmid

    #79538

    Purpose

    pRSETa (Invitrogen) plasmid expressing Amrose variant 2

    Depositor

    Randolph Caldwell

    Article

    Schoetz et al PLoS One. 2014 Sep 5;9(9):e107069. doi:

    Insert

    Amrose v2
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNB786

    Plasmid

    #60151

    Purpose

    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    LEU1pr

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNB812

    Plasmid

    #60160

    Purpose

    Yeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by RNR1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    yEVenus-PEST
    

    Use

    Tags

    PEST degron

    Expression

    Yeast

    Mutation

    Promoter

    RNR1pr

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mTFP1-SiT-N-15

    Plasmid

    #55508

    Purpose

    Localization: Golgi TGN, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    SiT (ST6GAL1 Human)
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    aa 1-45 of SiT

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

15. mTFP1-Talin-ABM-10

    Plasmid

    #55510

    Purpose

    Localization: Talin Actin Binding Domain, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    Talin ABM
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Actin Binding Module; I/LWEQ = 197 amino acids fr…

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-CAG-LiLac

    Plasmid

    #184570

    Purpose

    Fluorescent reporter for lactate (with lifetime and intensity response); expresses in mammalian cells and can be packaged as AAV

    Depositor

    Gary Yellen

    Article

    Koveal et al Nat Commun. 2022 May 25;13(1):2919. doi:

    Insert

    LiLac fluorescent sensor of lactate
    

    Use

    AAV

    Tags

    His7

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EGFP-Actin-7

    Plasmid

    #56421

    Purpose

    Localization: Actin, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Actin (ACTB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 23, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. PGK-H2BeGFP

    Plasmid

    #21210

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    Phosphoglycerate kinase promoter (PGK1 Human)
    

    Use

    Lentiviral

    Tags

    H2B-eGFP

    Expression

    Mutation

    Promoter

    Available Since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNCS-Antares

    Plasmid

    #74279

    Purpose

    Constitutive expression of Antares in bacteria

    Depositor

    Michael Lin

    Article

    Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    Antares
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mCherry-Actin-C-18

    Plasmid

    #54967

    Purpose

    Localization: Actin, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Actin (ACTB Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. GFP-C1-PKCgamma-C1A

    Plasmid

    #21205

    Depositor

    Tobias Meyer

    Article

    Oancea et al J Cell Biol. 1998 Feb 9. 140(3):485-98.

    Insert

    PKCgamma-C1A (Prkcg Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PKCgamma-C1A domain. (Lab plasmid WC 0018)

    Promoter

    Available Since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PGK-H2BmCherry

    Plasmid

    #21217

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    Phosphoglycerate kinase promoter (PGK1 Human)
    

    Use

    Lentiviral

    Tags

    H2B-mCherry

    Expression

    Mutation

    Promoter

    Available Since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEF-GalNAc-T2-msGFP2

    Plasmid

    #140164

    Purpose

    Expresses the Golgi marker GalnacT2-msGFP2

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    GalNAc-T2-msGFP2 (GALNT2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. lenti-CDDR

    Plasmid

    #149355

    Purpose

    CRISPR/Cas9-based dual-fluorescent DSB repair reporter

    Depositor

    Tarek Abbas

    Article

    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

    Inserts

    CDDR Reporter
    Blasticidin resistance
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a and hPGK

    Available Since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pC5Kan-P2A

    Plasmid

    #51814

    Purpose

    P2A peptide from porcine teschovirus-1 flanked by restriction sites in shuttle vector for easy cloning of multiple separate proteins to be expressed from single message

    Depositor

    Barry Ganetzky

    Article

    Daniels et al PLoS One. 2014 Jun 19;9(6):e100637. doi:

    Insert

    P2A peptide (PTV1gp1 Synthetic, porcine teschovirus-1)
    

    Use

    Shuttle vector for insect expression vectors

    Tags

    Expression

    Mutation

    codons altered for Drosophila and to eliminate re…

    Promoter

    Available Since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CMV-FlicR1

    Plasmid

    #74142

    Purpose

    Mammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicator

    Depositor

    Robert Campbell

    Article

    Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

    Insert

    FlicR1
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCF1148

    Plasmid

    #222330

    Purpose

    Fluorescent reporter plasmid for Veillonella

    Depositor

    J Christopher Fenno

    Article

    Goetting-Minesky et al Lett Appl Microbiol. 2024 Aug 5;77(8):ov

    Insert

    bs2 gene with Veillonella parvula SKV38 mdh promoter
    

    Use

    E. coli-veillonella shuttle plasmid

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Veillonella parvula SKV38 mdh promoter

    Available Since

    Oct. 31, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pSW003-Pc-mNeonGreen

    Plasmid

    #205018

    Purpose

    Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mNeonGreen (codon optimized for P. fluorescens)

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mNeonGreen is codon optimized for expression in P…

    Promoter

    Pc

    Available Since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. T/Brachyury-eGFP Rex Neo

    Plasmid

    #21222

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    Brachyury promoter
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available Since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GFP-pcDNA3-PKCgamma-cys1Acys1B

    Plasmid

    #21190

    Depositor

    Tobias Meyer

    Article

    Oancea et al J Cell Biol. 1998 Feb 9. 140(3):485-98.

    Insert

    PKCgamma-cys1Acys1B (Prkcg Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PKCgamma-cys1Acys1B (aa35 to aa152 of rat PKCgamm…

    Promoter

    Available Since

    July 15, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pNCS-CyOFP

    Plasmid

    #74278

    Purpose

    Constitutive expression of CyOFP in bacteria

    Depositor

    Michael Lin

    Article

    Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    CyOFP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMK1254

    Plasmid

    #133057

    Purpose

    Lentiviral vector with EF1A promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mRuby2 fusion protein.

    Depositor

    Martin Kampmann

    Article

    Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

    Insert

    Tau.K18(LM)
    

    Use

    Lentiviral

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSF-p15A-trc- YFP

    Plasmid

    #186569

    Purpose

    p15A ori, lacI, Ptrc promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

    Depositor

    Teresa de Diego Puente

    Article

    Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

    Insert

    YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc promoter

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMK1253

    Plasmid

    #133058

    Purpose

    Lentiviral vector with EF1A promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-Clover2 fusion protein.

    Depositor

    Martin Kampmann

    Article

    Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

    Insert

    Tau.K18(LM)
    

    Use

    Lentiviral

    Tags

    mClover2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGWKS9

    Plasmid

    #139416

    Purpose

    Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mTurquoise2 marker.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRSET-pr-Kaede (Kaede-A69T)

    Plasmid

    #99228

    Purpose

    Bacterial expression of the primed conversion capable protein pr-Kaede (Kaede-A69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-Kaede
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A69T for primed conversion applications

    Promoter

    T7

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. YFP-S100A10

    Plasmid

    #107200

    Purpose

    Expresses YFP-human S100A10 fusion protein for live cell imaging

    Depositor

    Ursula Rescher, Volker Gerke

    Article

    Zobiack et al J Cell Sci. 2002 Jan 1;115(Pt 1):91-8.

    Insert

    S100A10 (S100A10 Human)
    

    Use

    Tags

    Yellow Fluorescent Protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSW002-Pc-mNeonGreen

    Plasmid

    #205016

    Purpose

    Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mNeonGreen (codon optimized for P. fluorescens)

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mNeonGreen is codon optimized for expression in P…

    Promoter

    Pc

    Available Since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pQE32-pr-mEosFP (mEosFP-V69T)

    Plasmid

    #99213

    Purpose

    Bacterial expression of the primed conversion capable protein pr-mEosFP (mEosFP-V69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mEosFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    monomeric variant with mutation V69T for primed c…

    Promoter

    T5

    Available Since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSF-p15A-YFP

    Plasmid

    #186563

    Purpose

    p15A ori, promotorless, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

    Depositor

    Teresa de Diego Puente

    Article

    Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

    Insert

    YFP
    

    Use

    Tags

    None

    Expression

    Bacterial and Mammalian

    Mutation

    None

    Promoter

    Promotorless

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pRSET-pr-mEos3.2 (mEosFP3.2-A69T)

    Plasmid

    #99224

    Purpose

    Bacterial expression of the primed conversion capable protein pr-mEos3.2 (mEos3.2-A69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mEos3.2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A69T for primed conversion applications

    Promoter

    T7

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. mCyRFP1-RhoA

    Plasmid

    #84358

    Purpose

    fusion protein of RhoA, FRET donor

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mCyRFP1-RhoA (RHOA Human, Synthetic)
    

    Use

    Tags

    mCyRFP1 N terminal fusion to RhoA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pAAV EFIa DIO LMO11 (GeNL_SS-ChRmine)

    Plasmid

    #219426

    Purpose

    Cre-dependent expression of luciferase opsin fusion protein (luminopsin) for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Ikefuama et al iScience. 2025 Feb 13;28(3):112022. doi:

    Insert

    mNeonGreen, ISN-IE, ChRmine
    

    Use

    AAV

    Tags

    Gaussia luciferase signal sequence

    Expression

    Mutation

    Promoter

    EF-1alpha

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pEF-ManII-GFP

    Plasmid

    #160905

    Purpose

    Expresses the Golgi marker ManII-GFP in mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    MAN2A1 (MAN2A1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. EYFP-Mito-7

    Plasmid

    #56596

    Purpose

    Localization: Mitochondria, Excitation: 513, Emission: 527

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

46. pDL14

    Plasmid

    #106901

    Purpose

    pDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent manner

    Depositor

    Sergey Sineoky

    Article

    Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

    Inserts

    gam
    bet
    mutS
    lacI
    rhaR
    rhaS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lysine 622 was replaced by Alanine

    Promoter

    Available Since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. Fireworks GFP[PTC-] (AVA2598)

    Plasmid

    #85445

    Purpose

    Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.

    Depositor

    Joan Steitz

    Article

    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

    Insert

    tDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
    

    Use

    Minimal backbone fragment for low-copy bacterial …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pFlc245

    Plasmid

    #101021

    Purpose

    fluorescent, functional, glutamate receptor subunit

    Depositor

    Thomas Hughes

    Article

    Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

    Insert

    GluR 1 (Gria1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    fluorescent protein inserted into subunit

    Promoter

    CMV

    Available Since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. Fireworks RFP[PTC-] (AVA2515)

    Plasmid

    #85443

    Purpose

    Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.

    Depositor

    Joan Steitz

    Article

    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

    Insert

    tDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-HygromycinR
    

    Use

    Minimal backbone fragment for low-copy bacterial …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pBAD-SPW-RHH

    Plasmid

    #190630

    Purpose

    Bacterial expression of genetically encoded protease indicator SPW-RHH

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SPW-RHH
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge