We narrowed to 10,932 results for: AGA

1. L4440_BioBrick-SCR-sgRNA-B

   Plasmid

   #177812

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   Scrambled sgRNA targeting sequences
   

   Expression

   Bacterial

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDIV270

   Plasmid

   #226181

   Purpose

   Expresses Cas9 and gRNA targeting KU70 gene in K. phaffii.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

   Insert

   tRNA-sgRNA-tRNA
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Promoter

   TEF1p (Komagataella phaffii)

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCRIS

   Plasmid

   #120424

   Purpose

   This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3, IS5, and IS150.

   Depositor

   Tamás Fehér

   Article

   Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:

   Insert

   CRISPR spacers targeting IS1, IS5, IS3, IS150
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   constitutive

   Available Since

   Jan. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SuperNova/pRSETB

   Plasmid

   #53234

   Purpose

   monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation

   Depositor

   Takeharu Nagai

   Article

   Takemoto et al Sci Rep. 2013;3:2629. doi: 10.1038/srep0

   Insert

   monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation
   

   Tags

   6xHis, T7 tag, and Xpress tag

   Expression

   Bacterial

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. His-AgdNK

   Plasmid

   #203226

   Purpose

   For protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

   Depositor

   Vern Schramm

   Article

   Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

   Insert

   deoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)
   

   Tags

   N-terminal TEV cleavable 6xHis tag

   Expression

   Bacterial

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. shCDK4/6(1)

   Plasmid

   #73554

   Purpose

   vector encoding both shRNA against CDK4(1) and shRNA against CDK6(1)

   Depositor

   Gerardo Ferbeyre

   Article

   Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

   Insert

   shRNA against CDK4 + shRNA against CDK6
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pR1G108CP20X

   Plasmid

   #85086

   Purpose

   Expresses PCNA1 variant (G108C) fused to putidaredoxin reductase and putidaredoxin in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   The G108C variant of PCNA1 fused to putidaredoxin reductase and putidaredoxin
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   G108C in PCNA1

   Promoter

   T7 promoter

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PGL3-U6-SDHB_pegRNA-Csy4RS-nick_sgRNA-EGFP

   Plasmid

   #172670

   Purpose

   For expression of transcript containing pegRNA and nick-sgRNA targeting human SDHB gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

   Depositor

   Xiaolong Wang

   Article

   Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

   Insert

   SDHB pegRNA and SDHB_nick-sgRNA
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LEP-shMLL-AF9.1039

   Plasmid

   #105566

   Purpose

   retrovirally express MLL-AF9 shRNA with puro resistance and GFP marker

   Depositor

   Christopher Vakoc

   Article

   Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

   Insert

   shRNA targeting MLL part of MLL-AF9 fusion
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Promoter

   MSCV-LTR

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PGL3-U6-ALDOB_pegRNA-Csy4RS-nick_sgRNA-EGFP

    Plasmid

    #172668

    Purpose

    For expression of transcript containing pegRNA and nick-sgRNA targeting human ALDOB gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

    Insert

    ALDOB pegRNA and ALDOB_nick-sgRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PGL3-U6-RNF2_pegRNA-Csy4RS-nick_sgRNA-EGFP

    Plasmid

    #172669

    Purpose

    For expression of transcript containing pegRNA and nick-sgRNA targeting human RNF2 gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

    Insert

    RNF2 pegRNA and RNF2_nick-sgRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCF821-sgCIDE-44_U6-sgRNA-EF1a-mNeonGreen

    Plasmid

    #211684

    Purpose

    U6-sgRNA-EF1a-mNeonGreen

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    SpyCas9 and sgCIDE-44 guide RNA vector
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCF821-sgCIDE-41_U6-sgRNA-EF1a-mNeonGreen

    Plasmid

    #211681

    Purpose

    U6-sgRNA-EF1a-mNeonGreen

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    SpyCas9 and sgCIDE-41 guide RNA vector
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TRIN-E-shTAF12.364

    Plasmid

    #105572

    Purpose

    Dox-inducible mir30 MYB shRNA/dsRED expression with GFP marker and neo resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    TAF12 shRNA
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LENC-shTAF12.364

    Plasmid

    #105579

    Purpose

    retrovirally express TAF12 shRNA with Neo resistance and mCherry marker

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    TAF12 shRNA #364
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Promoter

    MSCV-LTR

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pC3R112C/T180C

    Plasmid

    #85088

    Purpose

    Expresses PCNA3 variant (R112C/T180C) fused to P450cam (CYP101A1) in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    The R112C/T180C variant of PCNA3 fused to P450cam
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C in PCNA3

    Promoter

    T7 promoter

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGS-3R112C/T180C

    Plasmid

    #85095

    Purpose

    Host vector to fuse a gene at the N-terminus of PCNA3 variant (R112C/T180C)

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    DNA polymerase sliding clamp 3
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    changed Arginine 112 to Cysteine and Threonine 18…

    Promoter

    T7 promoter

    Available Since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-HA-VprBP

    Plasmid

    #133586

    Purpose

    Expresses human VprBP wildtype in mammalian cells

    Depositor

    Yue Xiong

    Article

    Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

    Insert

    Vpr (HIV-1) binding protein (DCAF1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Wildtype human VprBP (Dcaf1)

    Available Since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p3R112C/T180CBM3

    Plasmid

    #85090

    Purpose

    Expresses PCNA3 variant (R112C/T180C) fused to P450 BM3 variant (A74G/C773S/C810S) in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    The R112C/T180C variant of PCNA3 fused to P450 BM3
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C in PCNA3, A74G/C773S/C810S in P450BM3

    Promoter

    T7 promoter

    Available Since

    Dec. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMSCV-GSDMD-Myc

    Plasmid

    #201166

    Purpose

    Expression of GSDMD gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

    Insert

    Gasdermin D (Gsdmd Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Promoter

    MESV

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge