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pSEVA6213S

Plasmid

#122094

Purpose

constitutive expression of I-SceI for genome engineering in Pseudomonas

Depositor

Pablo Ivan Nikel

Article

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

I-SceI restriction endonuclease

Promoter

Pem7

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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HA Display H3_A/Hong Kong/1/1968

Plasmid

#206506

Purpose

Expresses H3_Hong Kong/1/1968 in the surface of mammalian cells

Depositor

IIya Finkelstein

Insert

H3_A/Hong Kong/1/1968

Tags

3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

Expression

Mammalian

Mutation

deleted amino acids 1651 to 1735

Promoter

CMV

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pSEVA1213S

Plasmid

#122095

Purpose

constitutive expression of I-SceI for genome engineering in Pseudomonas

Depositor

Pablo Ivan Nikel

Article

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

I-SceI restriction endonuclease

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAG504-frFAST

Plasmid

#160055

Purpose

Expresses frFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

frFAST

Expression

Mammalian

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBBR5pemIKpKan

Plasmid

#129271

Purpose

Broad host-range plasmid with toxin-antitoxin system for maintenance in the absence of antibiotic selection, and constitutive promoter

Depositor

Lindsey Burbank

Article

Burbank et al Microbiol Resour Announc. 2019 Sep 5;8(3

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pFGL889

Plasmid

#52321

Purpose

Introducing ectopic integration at the defined fungal ILV2-locus, based on SRR (Sulfonylurea Resistance Reconstitution).

Depositor

Naweed Naqvi

Article

Yang et al Fungal Genet Biol. 2014 Apr 24. pii: S10

Type

Empty backbone

Use

Agrobacterium

Expression

Bacterial

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pSEVA63_sgRNA-B1_M13ps

Plasmid

#231422

Purpose

M13 phagemid that expresses sgRNA-B1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ615.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-B1

Use

Synthetic Biology

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-2_M13ps

Plasmid

#231412

Purpose

M13 phagemid that expresses sgRNA-2 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ171.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-2

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-A1_M13ps

Plasmid

#231414

Purpose

M13 phagemid that expresses sgRNA-A1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ164.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-A1

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pBsaG4SPdX

Plasmid

#108203

Purpose

G4S-PdX

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

Putidaredoxin

Expression

Bacterial

Mutation

changed Cysteine 73 to Serine and Cysteine 85 to …

Promoter

lac promoter

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV/SB11

Plasmid

#26552

Depositor

Perry Hackett

Article

Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

Insert

Sleeping Beauty

Expression

Bacterial

Mutation

Methionine 243 to Glutamine Threonine 136 to Arg…

Available Since

Oct. 25, 2010

Availability

Academic Institutions and Nonprofits only

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pSY6

Plasmid

#90482

Purpose

Targeted gene disruption by use of a group II intron (targetron) vector in Clostridium acetobutylicum

Depositor

Sheng Yang

Article

Shao et al Cell Res. 2007 Nov;17(11):963-5. doi: 10

Insert

group II intron and ItrA

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pSBbi Bla mAmetrine

Plasmid

#183257

Purpose

Stable expression of blue fluorescent mAmetrine protein in a Sleeping Beauty transposon vector.

Depositor

Alexander McLellan

Article

He et al Int J Mol Sci. 2020 Dec 4;21(23). pii: i

Insert

mAmetrime

Expression

Mammalian

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV/SB10ΔDDE

Plasmid

#24552

Depositor

Perry Hackett

Article

Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

Insert

Sleeping Beauty delta DDE

Expression

Bacterial

Mutation

removes 322 bp of the transposase-coding region

Promoter

CMV

Available Since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pIMP1-Pthl-AbrB3647

Plasmid

#80981

Purpose

Used for abrB3647(CAC3647) overexpression in Clostridium acetobutylicum

Depositor

Wei-Hong Jiang

Article

Xue et al Appl Microbiol Biotechnol. 2016 Jun 8.

Insert

abrB3647(CAC3647)

Expression

Bacterial

Promoter

thl (cac2873)

Available Since

Sept. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pIMP1-Pthl-AbrB1941

Plasmid

#80980

Purpose

Used for abrB1941(CAC1941) overexpression in Clostridium acetobutylicum

Depositor

Wei-Hong Jiang

Article

Xue et al Appl Microbiol Biotechnol. 2016 Jun 8.

Insert

abrB1941(CAC1941)

Expression

Bacterial

Promoter

thl (cac2873)

Available Since

Sept. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pIMP1-Pthl-AbrB0310

Plasmid

#80979

Purpose

Used for abrB0310(CAC0310) overexpression in Clostridium acetobutylicum

Depositor

Wei-Hong Jiang

Article

Xue et al Appl Microbiol Biotechnol. 2016 Jun 8.

Insert

abrB0310(CAC0310)

Expression

Bacterial

Promoter

thl (cac2873)

Available Since

Sept. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pBBR1k-con-GFPuv

Plasmid

#106386

Purpose

Constitutive trc promoter expressing gfpuv on BBR1 origin

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Expression

Bacterial

Promoter

trc

Available Since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEG03

Plasmid

#154138

Purpose

This plasmid carries fucO and aldA under the control of P_gyrA promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.

Depositor

Kang Zhou

Article

Panda et al Biochemical Engineering Journal

Insert

fucO, aldA

Expression

Bacterial

Promoter

P_gyrA

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-puroeGFP

Plasmid

#177922

Purpose

Constitutively expresses eGFP with puromycin selection

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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