We narrowed to 5,545 results for: donor

1. pR6K-Donor(PmcCAST)_KanR

   Plasmid

   #224928

   Purpose

   Transposition assay

   Depositor

   Leifu Chang

   Article

   Wang et al Methods Enzymol. 2025;712:55-79. doi: 10

   Insert

   LE_cargo_RE
   

   Expression

   Bacterial

   Available Since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSL3936 (pDonor(AAVS1)_Pse)

   Plasmid

   #200903

   Purpose

   Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a SA-T2A-PuroR-T2A-eGFP cargo. Total transposon size = 2,024 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   Mini Tn7016 (AAVS1 NGS)
   

   Expression

   Mammalian

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. CTCF-mAC donor (Hygro)

   Plasmid

   #140646

   Purpose

   CTCF tagging with mAID-clover

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   CTCF (CTCF Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. IVT-donor_opt(R2Tg)-GFP

   Plasmid

   #223246

   Purpose

   In vitro transcription of the optimized R2Tg RNA donor

   Depositor

   Wei Li

   Article

   Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

   Insert

   R2Tg donor_opt
   

   Use

   In vitro transcription

   Available Since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Bxb1-GT_AIO-TetOn_Divergent Donor

   Plasmid

   #229784

   Purpose

   Bxb1-GT donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   LacZ; rtTA-T2A-mTagBFP2
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE and CAG

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. Bxb1-GA_AIO-TetOn_Divergent Donor

   Plasmid

   #229786

   Purpose

   Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   LacZ; rtTA-T2A-mTagBFP2
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE and CAG

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Intron-Tagging-EGFP-Donor

   Plasmid

   #159740

   Purpose

   Contains EGFP flanked by a splice acceptor and a splice donor. Together with other intron tagging plasmids, it can be used to place the EGFP tag as a synthetic exon into introns of target genes.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

   Insert

   EGFP
   

   Use

   Intron tagging donor

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. ires-eGFP(+HAs) donor-1

   Plasmid

   #87865

   Purpose

   Express eGFP reporter for HDR-induced knock-in

   Depositor

   Bo Feng

   Article

   He et al Nucleic Acids Res. 2016 May 19;44(9):e85

   Insert

   eGFP
   

   Use

   Homologous recombination for gapdh loci

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDonor14-AcHELIX-KanR (CJT96)

   Plasmid

   #181794

   Purpose

   pDonor for AcHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   KanR, R6K origin of replication
   

   Expression

   Bacterial

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJMC-L1-Reverse-Donor-Esp3I

    Plasmid

    #197845

    Purpose

    Donor vector to restore L1 destination via Esp3I (reverse orientation)

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    None
    

    Use

    Unspecified

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS Donor mClover-LMNA

    Plasmid

    #98970

    Purpose

    Donor for endogenous N-ter tagging of hLMNA with mClover. Cas9 Homologous Recombination Reporter

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA5'UTR-mClover-LMNA5'exon1 (LMNA Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. SMC2-mAC donor (Hygro)

    Plasmid

    #140648

    Purpose

    SMC2 tagging with mAID-Clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    SMC2 (SMC2 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. mini-donor - SOX17-EGFP

    Plasmid

    #206048

    Purpose

    this mini-donor knockin vector can be used to knock EGFP into SOX17 locus

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

    Insert

    nuclear EGFP
    

    Use

    Knockin donor vector

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDonor-tBFP-NLS-Neo

    Plasmid

    #80766

    Purpose

    Customizable donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    TagBFP2-3×NLS

    Expression

    Mammalian

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. mini-donor - NKX6.1-nEGFP

    Plasmid

    #206047

    Purpose

    this mini-donor knockin vector can be used to knock nuclear EGFP into NKX6.1 locus

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

    Insert

    nuclear EGFP
    

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCVL SFFV d14GFP Donor

    Plasmid

    #31475

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV d14GFP
    

    Use

    Lentiviral

    Mutation

    Deleted 14 amino acids on the c terminus of eGFP

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHiUGE HA-epitope donor ORF1

    Plasmid

    #200372

    Purpose

    Tagging endogenously expressed proteins with HA epitope, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Insert

    HA epitope
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. mAC-POLR2A donor (Hygro)

    Plasmid

    #124496

    Purpose

    A donor plasmid for tagging the N-terminus of human POLR2A with mAID-mClover

    Depositor

    Masato Kanemaki

    Article

    Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

    Insert

    POLR2A homology arms and Hygro-P2A-mAID-mClover (POLR2A Human)
    

    Use

    CRISPR

    Tags

    mAID-mClover

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSL3927 (pDonor(plasmid)_Pse)

    Plasmid

    #200905

    Purpose

    Encodes a mini-transposon derived from Pseudoalteromonas Tn7016,with a CmrR cargo, as well as a primer binding site for deep sequencing purposes at pTarget. Total transposon size = 1,087 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    Mini Tn7016 (pTarget NGS)
    

    Expression

    Mammalian

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAF0688 Kp03_attP-mCherry donor

    Plasmid

    #193467

    Purpose

    Promoterless mcherry donor for Kp03

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Kp03_attP
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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