Addgene

We narrowed to 14,497 results for: pET

Showing: 1991 - 2000 of 14497 results

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pET21d-colEwt2/Imwt2

Plasmid

#107127

Purpose

plasmid for expression of WT colicin endonuclease and immunity pair 2

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEwt2/Imwt2

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-ART

Plasmid

#174810

Purpose

Bacterial expression of isolated PARP1 ART subdomain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-ART (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-BRCT

Plasmid

#174807

Purpose

Bacterial expression of isolated PARP1 BRCT domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-BRCT (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-WGR

Plasmid

#174808

Purpose

Bacterial expression of isolated PARP1 WGR domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-WGR (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-MinE-His

Plasmid

#133623

Purpose

Expression vector for the Escherichia coli MinE with an C-terminal fusion of a His-tag for purification.

Depositor

Petra Schwille

Article

Glock et al ACS Synth Biol. 2018 Dec 20. doi: 10.102

Insert

MinE-His (minE E. coli)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-29

Plasmid

#172753

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-29; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-29

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET15b I-SceI (Ctermhis -MH)

Plasmid

#195613

Purpose

Expresses the wild-type I-SceI homing endonuclease from the T7 promoter

Depositor

Frederick Gimble

Article

Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

Insert

I-SceI

Use

Tags

Expression

Bacterial

Mutation

None

Promoter

T7

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET28b-BE3∆UGI

Plasmid

#96943

Purpose

The plasmid encoding the His6-rAPOBEC1-XTEN-nCas9 protein (BE3∆UGI) was generated by site-directed mutagenesis using pET28b-BE1 (Addgene plasmid #73018).

Depositor

Daesik Kim

Article

Kim et al Nat Biotechnol. 2017 May;35(5):475-480.

Insert

BE3∆UGI

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pET-22b(+)-UTPase

Plasmid

#12667

Depositor

SeWon Suh

Article

Han et al Acta Crystallogr D Biol Crystallogr. 200

Insert

dUTP pyrophosphatase (DUT1 Budding Yeast)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pET28a His6Flag2AUF1(p37)

Plasmid

#135939

Purpose

Expresses AUF1p37 in E. coli

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Use

Tags

Flag2 and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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