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PpyCAG-NANOG-IB

Plasmid

#60440

Purpose

Transient human NANOG expression

Depositor

Austin Smith

Article

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Insert

NANOG (NANOG Human)

Expression

Mammalian

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

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tdp43-EGFP construct6

Plasmid

#28199

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa1-314 only

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
tdp43-EGFP construct2

Plasmid

#28195

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa86-414 only

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
pBItetDT12nGFP

Plasmid

#80420

Purpose

tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 12 CTG repeats in exon 15 located

Depositor

Thomas Cooper

Article

Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

Insert

EGFP and human DMPK exons 11-15 with 12 CTG repeats (DMPK Human)

Expression

Mammalian

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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EK0493 SFFV-mCherry-SGc(GAPDH.s1)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#191156

Purpose

Expression of a sensor for GAPDH 3' UTR in mammalian cells; mCherry marker, EGFP output, three MS2 sequences in 3' UTR

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:GAPDH.s1:EGFP-3xMS2

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pEF-ManII-GFP

Plasmid

#160905

Purpose

Expresses the Golgi marker ManII-GFP in mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

MAN2A1 (MAN2A1 Human)

Tags

GFP

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pACT2-CAS9c

Plasmid

#51055

Purpose

Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.

Depositor

Yunde Zhao

Article

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

Cas9c

Use

CRISPR

Tags

SV40 NLS

Expression

Bacterial and Yeast

Promoter

yeast ADH1 promoter

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pTPH413

Plasmid

#185728

Purpose

Bacterial PAM profiling of adenine base editor variants

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Inserts

ecTadA(8e)-neNme2-C
Nme2Cas9 sgRNA

Use

CRISPR

Expression

Bacterial

Mutation

Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe Angptl4 puro

Plasmid

#19156

Depositor

Joan Massague

Article

Padua et al Cell. 2008 Apr 4. 133(1):66-77.

Insert

angiopoietin-like 4 (ANGPTL4 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only
tdp43-EGFP construct7

Plasmid

#28200

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa1-274 only

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
p2T-cmv-SpCas9-TadDE-BlastR

Plasmid

#193850

Purpose

Express TadDE in mammalian cells with BlastR selection, Tol2 integration

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadDE

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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tdp43-EGFP construct5

Plasmid

#28198

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa257-414 only

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-EFS-fDIO-GRAB_eCB2.0

Plasmid

#164610

Purpose

Express the endocannabinoid sensor GRAB_eCB2.0 in FLP positive cells

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid sensor GRAB_eCB2.0

Use

AAV

Mutation

S383T

Promoter

EFS

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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Flag Intersectin Long (human)

Plasmid

#47393

Depositor

Peter McPherson

Article

Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

Insert

intersectin long (ITSN1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO V5 DNAJB6a

Plasmid

#19529

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB6a (DNAJB6 Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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tdp43-EGFP construct8

Plasmid

#28201

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa275-313 deleted

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-DIO-GRAB_eCB2.0mut

Plasmid

#164607

Purpose

Express the endocannabinoid non-responsive sensor GRAB_eCB2.0 in Cre positive neurons

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid non-responsive sensor GRAB_eCBmut

Use

AAV

Mutation

S383T, F177A

Promoter

hSyn

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLUEF1 Luc2-IRES-GFP

Plasmid

#224467

Purpose

Ef1a downstream of a ubiquitous chromatin opening element drives expression of Luciferase2 and IRES GFP

Depositor

Robert Kerbel

Article

Khan et al Cancer Res. 2025 Apr 14. doi: 10.1158/00

Insert

Luciferase

Use

Lentiviral

Promoter

EF1a

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pJT396 PB ITR and Cp36 attD pEF Puro-2a-tagBFP2 donor

Plasmid

#184952

Purpose

Donor for both PiggyBac and Cp36 Recombinase

Depositor

Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

PuroR, P2A, mTagBFP2

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Cas12f-GE ver4.0

Plasmid

#176543

Purpose

Expresses Cas12f-GE in mammalian cells

Depositor

Yong-Sam Kim

Article

Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

Inserts

Cas12f-GE ver4.0
Cas12f-GE ver4.0

Expression

Mammalian

Promoter

U6 and chicken β-actin

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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