Addgene

We narrowed to 8,073 results for: gnal

Showing: 5971 - 6000 of 8073 results

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pZSm45 ndhII

Plasmid

#48889

Purpose

Lux-regulated expression of NDH-2 enzyme

Depositor

Jeff Hasty

Article

Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

Insert

ndhII and LuxR

Use

Synthetic Biology

Tags

ssrA tag (AANDENYALAA)

Expression

Bacterial

Mutation

Promoter

pLux

Available since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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NLS-LucDM-GFP

Plasmid

#127796

Purpose

The expressed ORF encodes nucleus location signal with GFP and destabilized mutant Luciferase.

Depositor

Xiaolu Yang

Article

Guo et al J Vis Exp. 2016 Aug 28;(114). doi: 10.37

Insert

destabilized mutant (DM) Luciferase

Use

Tags

EGFP and FLAG-NLS

Expression

Mammalian

Mutation

R188Q, R261Q in Firefly Luciferase insert

Promoter

CMV

Available since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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pACYC-PLlambda-alkS(WT)

Plasmid

#44382

Depositor

Hal Alper

Article

Reed et al J Biotechnol. 2012 Apr 15;158(3):75-9. d

Insert

AlkS(WT)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PLlambda

Available since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pAG413 GAL ORF1 Shuffle1 NLS

Plasmid

#145794

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock

Article

Payero et al Journal of the South Carolina Academy of

Insert

ORF1 Shuffle1 NLS

Use

Tags

Expression

Yeast

Mutation

Enhanced Nucleur Localization Signal

Promoter

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pTD103luxI_sfGFP -R

Plasmid

#48887

Purpose

Positive feedback plasmid from oscillator without luxR

Depositor

Jeff Hasty

Article

Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

Insert

sfGFP and luxI

Use

Synthetic Biology

Tags

ssrA tag (AANDENYALAA)

Expression

Bacterial

Mutation

Promoter

pLux

Available since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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mOrange2-Peroxisomes-2

Plasmid

#54596

Purpose

Localization: Peroxisomes, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Type

Empty backbone

Use

Tags

Peroximal Targeting Signal 1 (PTS1) and mOrange2

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2014

Availability

Academic Institutions and Nonprofits only
pPTK005-3a-αMF

Plasmid

#84964

Purpose

Type 3a, α-mating factor - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

α-mating factor secretion tag

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Non

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCWU6-xylR-E-lepB

Plasmid

#190725

Purpose

fine tune expression of type 1 signal peptidase under the control of xylose- and theophylline- inducible system

Depositor

Chenggang Wu

Article

Bibek et al bioRxiv. 2023 Apr 24:2023.04.24.538132.

Insert

xylR-E-lepB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDJSVT89

Plasmid

#109303

Purpose

Express periplasmic, outer membrane, or secreted proteins in E. coli - TamA signal and His6 tag

Depositor

Daniel Slade

Article

Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

Type

Empty backbone

Use

Tags

TamA 1-27, 6His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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NK644

Plasmid

#109096

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_00306 geranyl-geranylation signal

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pDJSVT90

Plasmid

#109304

Purpose

Express periplasmic, outer membrane, or secreted proteins in E. coli - TamA signal and His6 tag

Depositor

Daniel Slade

Article

Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

Type

Empty backbone

Use

Tags

TamA 1-27, 6His

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA_DiCre2_59.F2

Plasmid

#60209

Purpose

Expresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(19-59)

Depositor

Jean-Paul Herman

Insert

Cre recombinase (CD207 Bacteriophage P1, Human, Synthetic)

Use

Tags

FKBP12(F36V) and Nuclear localisation signal

Expression

Mammalian

Mutation

FKBP12 has the F36V mutation; amino acids 19-59 o…

Promoter

CMV

Available since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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GPI-mGreenLantern

Plasmid

#197916

Purpose

Monomer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-mGreenLantern

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pTD103aiiA(Cm) -R

Plasmid

#48888

Purpose

Negative feedback plasmid from oscillator without luxR

Depositor

Jeff Hasty

Article

Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

Insert

aiiA

Use

Synthetic Biology

Tags

ssrA tag (AANDENYALAA)

Expression

Bacterial

Mutation

Promoter

pLux

Available since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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myr-palm-mEGFP(3x)

Plasmid

#182872

Purpose

Homo-trimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al Biophys J. 2021 Dec 21;120(24):5478-5490

Insert

myr-palm-mEGFP(3x)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pZSm45 sodA

Plasmid

#48890

Purpose

Lux-regulated expression of sodA enzyme

Depositor

Jeff Hasty

Article

Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

Insert

sodA LuxR

Use

Synthetic Biology

Tags

ssrA tag (AANDENYALAA)

Expression

Bacterial

Mutation

Promoter

pLux

Available since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pJT103

Plasmid

#25087

Depositor

Christopher Voigt

Article

Tabor et al Cell. 2009 Jun 26. 137(7):1272-81.

Insert

PompC-lacZ

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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366 pTol2-CherryDest

Plasmid

#73487

Purpose

promoterless Tol2 vector with mCherry tag upstream of and in frame with attR1/attR2 cassette and late SV40 polyadenylation signal

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

mCherry-attR1/attR2

Use

Tags

Expression

Mutation

H30R (please see depositor comments below)

Promoter

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pFF746

Plasmid

#61451

Purpose

aTc-inducible SCRIBE_galK(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_galK(ON)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pTetO

Available since

March 6, 2015

Availability

Academic Institutions and Nonprofits only

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pFF828

Plasmid

#61456

Purpose

AHL-inducible msd_lacZ(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd_lacZ(ON)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pLuxR

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCS-PesaR-Plux

Plasmid

#47655

Purpose

PesaR-P promoter in pCS26

Depositor

Cynthia Collins

Article

Shong et al ACS Synth Biol. 2013 Jul 23.

Insert

PesaR-P

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Added an additional esa box downstream of the ori…

Promoter

PesaR-P

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only
pMH186

Plasmid

#122837

Purpose

MS2-NLS (no stop codon) with GreenGate C-D flanking sequences

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

MS2-NLS, codon-optimised for Arabidopsis

Use

Golden gate compatible cloning vector

Tags

Nuclear localisation signal

Expression

Mutation

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAWp7-3

Plasmid

#72735

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-[miR-132]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-CMVp-GFP-[miR-132]

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-7

Plasmid

#72739

Purpose

pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-124 sensor]-CMVp-GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pJexpress414_OmpA171_H1237

Plasmid

#64290

Purpose

OmpA redesign hybrid with wild-type strands 1237

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_H1237

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_H-3

Plasmid

#64310

Purpose

OmpA redesign hybrid with wild-type strands 1245678

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_H-3

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_R4cons_G41A

Plasmid

#64299

Purpose

Conservative version of OmpA Redesign 4 with G41A mutation

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_R4cons_G41A

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pICH77901

Plasmid

#50341

Depositor

Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, mas (A. tumefaciens)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pJexpress414_OmpA171_R4cons

Plasmid

#62639

Purpose

Conservative version of OmpA Redesign 4

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_R4cons

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Mutation

25 mutations to lipid-facing surface

Promoter

T7

Available since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pGST1-STAM1-UIM

Plasmid

#44425

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

STAM1-UIM (STAM Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only