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G-GECO1.2-Orai1Y80E

Plasmid

#73565

Purpose

Green fluorescent reporter of mutant Orai1Y80E-associated calcium influx

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

G-GECO1.2-Orai1Y80E (ORAI1 Human, Synthetic)

Tags

G-GECO1.2

Expression

Mammalian

Mutation

changed tyrosine 80 of Orai1 to glutamate

Promoter

CMV Immediate Early

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-short Cre-on G/W

Plasmid

#86949

Purpose

Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. For introducing large constructs into AAV

Depositor

Joseph Dougherty, Rob Mitra

Article

Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pInducer10-UBC9-sh4

Plasmid

#168988

Purpose

Expresses RFP cDNA and UBC9 shRNA 4 in mammalian cells

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Ubiquitin Conjugating Enzyme 9

Expression

Mammalian

Promoter

Ubc promoter

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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Nanog-luciferase targeting construct

Plasmid

#26591

Depositor

Rudolf Jaenisch

Article

Lyssiotis et al Proc Natl Acad Sci U S A. 2009 Jun 2. 10

Insert

Nanog promoter-luciferase-lox-pgk-neomycin-loc

Expression

Bacterial

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SNORD27h

Plasmid

#73066

Purpose

expression clone for human SNORD27 (C/D box snoRNA U27)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD27 (SNORD27 Human)

Tags

no tag

Expression

Mammalian

Mutation

expression cassette for SNORD27

Promoter

CMV

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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mitoTALECD for_atp1_1397CN

Plasmid

#186203

Purpose

For genome editing (base editing) of Arabidopsis mitochondria, targeted C of RNA editing site of ATP1 by OTP87

Depositor

Shin-ichi Arimura

Article

Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11

Inserts

mitochondrial targeted TALE left hand with 1397C of Cytidine Deaminase (mitoTALECD)
mitochondrial targeted TALE right hand with 1397N of Cytidine Deaminase (mitoTALECD)

Use

Synthetic Biology; Ti plasmid for plant transform…

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBRPyCAG-fmKlf4-DsRed-Ip

Plasmid

#26274

Depositor

Rudolf Jaenisch

Article

Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

Insert

Klf4 (Klf4 Mouse)

Use

Cre/Lox

Expression

Mammalian

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

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His-GST-Cys-M87 Spastin

Plasmid

#184804

Purpose

Bacterial expression of M87 Spastin (A2C mutation)

Depositor

James Hurley

Article

Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

Insert

M87 Spastin (SPAST Human)

Tags

His6-GST

Expression

Bacterial

Mutation

A2C

Promoter

T7

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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HOXB7 (human) pCMV

Plasmid

#8539

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB7 (HOXB7 Human)

Tags

FLAG

Expression

Mammalian

Mutation

starts at AA#1 preceeded by FLAG and Ser-Arg-Ile…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pDN-G1TGt

Plasmid

#44508

Depositor

Gabor Balazsi

Article

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
tetR::yEGFP

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Two tandem tet operators (2XtetO2) downstream of …

Promoter

PGal1-D12 promoter

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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GFP-CLCb (EED/QQN)

Plasmid

#47422

Depositor

Peter McPherson

Article

Poupon et al Proc Natl Acad Sci U S A. 2008 Jan 8;105

Insert

CLCb EED/QQN (CLTB Human)

Tags

EGFP

Expression

Mammalian

Mutation

EED changed to QQN, in aa20-41 region

Promoter

CMV

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
PB-hSyn-mSyp-RT1-24xGCN4

Plasmid

#106300

Purpose

The plasmid encodes mouse Synaptophysin sparsely labeled with SunTag24x_v4.

Depositor

James Zhe Liu

Article

Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p

Insert

mSyp-RT1-24xGCN4 (Syp Mouse)

Tags

24xGCN4

Expression

Mammalian

Promoter

human Synapsin1

Available Since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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HOXB4 (human) FLAG MSCV

Plasmid

#8528

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB4 (HOXB4 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Missing N-terminal 14 amino acids; improved Ribos…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pGL3-IRES-Lin28b-P3

Plasmid

#64794

Purpose

Luciferase expression driven by Lin28b promoter P3

Depositor

Joshua Mendell

Article

Chang et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Lin28b promoter P3 (LIN28B Human)

Use

Luciferase

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5-H1-shRNA-CMV-HA-PACSIN1

Plasmid

#72577

Purpose

Bicistronic construct expressing PACSIN1 shRNA and HA-PACSIN1-rescue construct

Depositor

Richard Huganir

Article

Anggono et al Proc Natl Acad Sci U S A. 2013 Aug 20;11

Inserts

PACSIN1 (Pacsin1 Mouse)
H1-PACSIN1 shRNA cassette

Use

RNAi

Tags

HA

Expression

Mammalian

Mutation

5 silent mutations around shRNA recognition seque…

Promoter

CMV and H1

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV4Neo-murine IL-17RASEFIR-HA

Plasmid

#46863

Purpose

expresses mouse IL-17RA with SEFIR deletion

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

IL-17RA-delta-SEFIR (Il17ra Mouse)

Tags

3x HA

Expression

Mammalian

Mutation

Deletion of SEFIR domain (AA 394-485)

Promoter

CMV

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
pSG5-FLAG-mEKLF

Plasmid

#67833

Purpose

Expression of FL-EKLF driven by SV40 promoter

Depositor

James Bieker

Article

Soni et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

EKLF (Klf1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Full-length mEKLF is aa 20-376; amino acid 19 is …

Promoter

SV40

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only
J23117-mRFP1-331Bb

Plasmid

#78280

Purpose

mRFP1 behind constitutive promoter J23117 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23117-mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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PA-DreN

Plasmid

#169707

Purpose

The optimized DreN246-nMag

Depositor

Dali Li

Article

Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

Insert

Dre246-nMag

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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PA-DreC

Plasmid

#169705

Purpose

The optimized pMag-Dre247

Depositor

Dali Li

Article

Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

Insert

pMag-Dre247

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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