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pSLIK-Rbfox2-rABE-Flag

Plasmid

#191384

Purpose

Doxycycline inducible expression of Rbfox2-rABE

Depositor

Stephen Floor

Article

Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

Insert

Rbfox2-rABE (RBFOX2 Synthetic, Human)

Use

Lentiviral

Tags

Flag

Promoter

tet-inducible promoter

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pUTKan-3xHA-GFP-TOM5

Plasmid

#130674

Purpose

Ubiquitously expresses 3xHA-GFP-TOM5 in the outer mitochondrial membrane of planta

Depositor

Andreas Weber

Article

Kuhnert et al Plant Physiol. 2020 Feb;182(2):692-706.

Insert

Translocase of outer mitochondrial membrane 5 (TOM5 Mustard Weed)

Tags

3xHA and sGFP

Expression

Plant

Promoter

Ubiquitin10

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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retro-gRNA-mRFP1

Plasmid

#112914

Purpose

Retrovirus expressing mRFP1 with BBSI cloning sites for sgRNA

Depositor

Christophe Benoist, Diane Mathis

Article

Magnuson et al Proc Natl Acad Sci U S A. 2018 Nov 6;115

Insert

mRFP1

Use

Retroviral

Promoter

EF1a

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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MAC3-FGFR1

Plasmid

#222611

Purpose

Expression of MAC3-tagged FGFR1

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

FGFR1 (FGFR1 Human)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pET-30a-METTL17-8XHIS

Plasmid

#215705

Purpose

Express METTL17-8XHIS in C41(DE3) cells

Depositor

Vamsi Mootha

Article

Ast et al Mol Cell. 2024 Jan 18;84(2):359-374.e8.

Insert

METTL17 (METTL17 Human)

Tags

HIS

Expression

Bacterial

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Adeno EA

Plasmid

#64071

Purpose

Adenovirus for the expression of gRNAs targeting intron 19 of murine Alk and intron 14 of Eml4

Depositor

Andrea Ventura

Article

Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

Insert

U6_sgRNA(Alk)_U6_sgRNA(Eml4)_CBh_FLAG-Cas9

Use

Adenoviral

Tags

FLAG-Cas9

Promoter

U6 and CBh

Available Since

July 23, 2015

Availability

Academic Institutions and Nonprofits only
T/Brachyury-eGFP Rex Neo

Plasmid

#21222

Depositor

Mark Mercola

Article

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

Brachyury promoter

Use

Lentiviral

Tags

eGFP

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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pC081: pAAV.CMV.mCherry

Plasmid

#205744

Purpose

AAV vector for expressing CMV-driven mCherry reporter gene.

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Insert

mCherry

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-SIRT2-Flag

Plasmid

#102624

Purpose

Expresses full length SIRT2 isoform 1 in mammalian cells

Depositor

Hening Lin

Article

Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

Insert

SIRT2 (SIRT2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE

Plasmid

#138145

Purpose

Expresses PspCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI.

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

PspCas13b

Use

Lentiviral

Tags

NLS

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pDF0317 HvsCas7-11 mammalian expression

Plasmid

#172511

Purpose

Encodes human codon optimized HvsCas7-11 protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

HvsCas7-11 mammalian expression

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-IRES-Puro GFP-3xFLAG

Plasmid

#201636

Purpose

Over-expression of 3xFLAG-tagged GFP

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

GFP

Use

Lentiviral

Tags

3xFLAG

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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SiC-V2-Cas9G7

Plasmid

#133043

Purpose

Doxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.

Depositor

Sriram Neelamegham

Article

Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

Insert

Tet repressor

Use

Lentiviral

Tags

Cerulean

Available Since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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hTPST2

Plasmid

#11253

Depositor

Hyeryun Choe

Article

Choe et al Cell. 2003 Jul 25. 114(2):161-70.

Insert

TPST2 (TPST2 Human)

Tags

C9 (GTETSQVAPA)

Expression

Mammalian

Available Since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only

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CRY2PHR(W349R)-mCherry

Plasmid

#75370

Purpose

Expresses fusion of Arabidopsis CRY2 (residues 1-498, W349R fast-cycling mutant) fused to mCherry

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CRY2 residues 1-498 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Mutation

W349R

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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W118-1_hTGM2-V5

Plasmid

#180413

Purpose

lentiviral transduction of human TGM2 transcript variant 2 (with a C-terminal V5 tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM2 (TGM2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA SF-HERC2 F6

Plasmid

#55789

Purpose

Expresses N-terminal 3xFLAG/STREP tagged HERC2 (4421-4834aa)

Depositor

David Chan

Article

Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

Insert

3xFLAG tagged HERC2 (4421-4834aa) (HERC2 Human)

Tags

3xFLAG and STREP

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2014

Availability

Academic Institutions and Nonprofits only
pRS415-TEF1pr-MFA-NFAPoptim

Plasmid

#221090

Purpose

FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ER

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a-C2-mKate

Plasmid

#208763

Purpose

To express C2-mKate in bacteria

Depositor

Urte Neniskyte

Article

Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

Insert

C2-mKate (Mfge8 Mouse)

Tags

mKate

Expression

Bacterial

Promoter

T7 promoter

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRM3-cpHalo∆-mEGFP

Plasmid

#194678

Purpose

CMV driven expression of the m3 GPCR recorder in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

CHRM3_cpHalo∆

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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