We narrowed to 22,071 results for: his

1. HLTV-hTRIM21

   Plasmid

   #104973

   Purpose

   Purification of His-Lipoyl-hTRIM21 protein from bacteria

   Depositor

   Leo James

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   Tripartite Motif containing 21 (TRIM21 Human)
   

   Use

   Tags

   His-Lipoyl

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. FnR8-10

   Plasmid

   #182569

   Purpose

   Human fibronectin fragment, type-III repeats 8-10, R1379A mutant

   Depositor

   Timothy Springer

   Article

   Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

   Insert

   Human fibronectin fragment, type-III repeats 8-10
   

   Use

   Tags

   His and TEV cleavage site after His tag

   Expression

   Bacterial

   Mutation

   R1379A

   Promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMAL-c5x_UnaG

   Plasmid

   #163125

   Purpose

   A plasmid with UnaG coding sequence cloned into it. UnaG gene codon optimized for E. coli and B. theta. Expresses UnaG with N-term maltose-binding protein (MBP)-tag and C-term His-tag.

   Depositor

   Julie Biteen

   Article

   Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi

   Insert

   UnaG
   

   Use

   Tags

   His and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-beta-3

   Plasmid

   #27289

   Depositor

   Timothy Springer

   Article

   Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

   Insert

   integrin beta 3 (ITGB3 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   Available since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCN-mBFP

   Plasmid

   #63847

   Purpose

   Encodes a NADPH dependent blue fluorescent protein expressed under a strong promoter and strong RBS without His-tagged.

   Depositor

   Howard Salis

   Article

   Ng et al Metab Eng. 2015 May;29:86-96. doi: 10.10

   Insert

   mBFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter and RBS was swapped. His-tag was removed…

   Promoter

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBad-mCherry-XL (R125K)

   Plasmid

   #188054

   Purpose

   Expresses His-tagged, Arabinose-inducible mCherry-XL (R125K) in bacteria

   Depositor

   Ralph Jimenez

   Article

   Mukherjee et al J Phys Chem B. 2022 Jun 16. doi: 10.1021

   Insert

   mCherry-XL (R125K)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   differs from mCherry by R125K, W143S, I161V, Q163…

   Promoter

   araBAD

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET30-RT1306

   Plasmid

   #131521

   Purpose

   Expresses engineered RT-1306 with His-tag

   Depositor

   Bryan Dickinson

   Article

   Zhou et al Nat Methods. 2019 Dec;16(12):1281-1288.

   Insert

   RT-1306
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. FnR9-10

   Plasmid

   #182567

   Purpose

   Human fibronectin fragment, type-III repeats 9-10, R1379A mutant

   Depositor

   Timothy Springer

   Article

   Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

   Insert

   Human fibronectin fragment, type-III repeats 9-10
   

   Use

   Tags

   His and TEV cleavage site after His tag

   Expression

   Bacterial

   Mutation

   R1379A

   Promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBEL1284

   Plasmid

   #175960

   Purpose

   For expression of LetA-LetB operon from E. coli. N-terminal his tag on LetA, araBAD promoter

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

   Inserts

   LetA
   LetB
   

   Use

   Tags

   6xHis 2xQH and TEV cleavage site on LetA

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 myc TSC1

    Plasmid

    #12133

    Depositor

    Cheryl Walker

    Article

    Cai et al J Cell Biol. 2006 Apr 24. 173(2):279-89.

    Insert

    TSC1 (TSC1 Human)
    

    Use

    Tags

    his and myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBudORF1-CH

    Plasmid

    #51290

    Purpose

    expresses the codon optimized ORF1 of L1RP with his tag

    Depositor

    Astrid Roy-Engel

    Article

    Wagstaff et al Mol Biol Evol. 2013 Jan;30(1):88-99. doi

    Insert

    L1 ORF1 (L1RE1 Human)
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-SpySrtA

    Plasmid

    #51139

    Purpose

    expression plasmid for N-terminal his tagged S.pyogenes SrtA

    Depositor

    Hidde Ploegh

    Article

    Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

    Insert

    S.pyogenes SrtA
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-80

    Promoter

    T7

    Available since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3 V5 hHR23A

    Plasmid

    #13053

    Depositor

    Steven Grossman

    Article

    Brignone et al Oncogene. 2004 May 20. 23(23):4121-9.

    Insert

    hHR23A (RAD23A Human)
    

    Use

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    R325K and L355P relative to NP_001257291.1

    Promoter

    Available since

    Oct. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Plasmid L5 attB::Pleft* mCherry

    Plasmid

    #169411

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mCherry

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    pLeft
    mCherry
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJPT003-PM-HaloTag-1x FLAG

    Plasmid

    #202494

    Purpose

    Express "PM" HaloTag

    Depositor

    Neha Kamat

    Article

    Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

    Insert

    HaloTag
    

    Use

    Tags

    1x FLAG and Palmitoylation-Myristoylation Site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Plasmid L5 attB:Psmyc double blue

    Plasmid

    #170050

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double blue

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    mTagBFP2
    CO BFP
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPD128 AND Gate, 3 repeats

    Plasmid

    #138937

    Purpose

    EYFP reporter vector for the Logic 37 AND 158 NOT 43 3 repeats promoter

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    Logic 37 AND 158 NOT 43 3 repeats
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Logic 37 AND 158 NOT 43 3 repeats

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NLSx3-CRY2PHR-p65

    Plasmid

    #64124

    Purpose

    Photoactivatable transcription system. Expression of activator probe, which includes the photolyase homology region of CRY2 (CRY2PHR) and the transcriptional activator domain.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    CRY2PHR-p65
    

    Use

    Tags

    His, NLSx3, and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Plasmid L5 attB:Psmyc double green

    Plasmid

    #169412

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double green

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    mWasabi
    mWasabi CO
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Plasmid L5 attB:Psmyc triple red

    Plasmid

    #169413

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for triple red

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    mScarlet proper
    COmScarlet
    mRuby3
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. RNF146-c005

    Plasmid

    #173091

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    RNF146 (RNF146 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    R99-L183, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pIBA3-SpyCatcher-sfGFP

    Plasmid

    #107420

    Purpose

    For production of SpyCatcher protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.

    Depositor

    Jack Leo

    Article

    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

    Insert

    SpyCatcher
    

    Use

    Tags

    His tag, StrepII, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. GDAP2-c005

    Plasmid

    #173110

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    GDAP2 (GDAP2 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    M1-N231

    Promoter

    Available since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mH2A1.1-CT-MYC

    Plasmid

    #45166

    Depositor

    Brian Chadwick, Hunt Willard

    Article

    Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

    Insert

    H2Afy (MACROH2A1 Human)
    

    Use

    Tags

    HIS and MYC

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTE5400_pMBP_CDS dropout vector

    Plasmid

    #221189

    Purpose

    Expression vector for N-terminally His-tagged maltose binding protein (10xHis-MBP) fused to a protein of interest

    Depositor

    Tobias Erb

    Article

    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

    Type

    Empty backbone

    Use

    Tags

    10x His-tag + E. coli maltose binding protein (MB…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pWaldo-GFPe-SH1446

    Plasmid

    #112507

    Purpose

    Expresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag.

    Depositor

    Simon Newstead

    Article

    Minhas et al Elife. 2018 Jul 3;7. pii: 34995. doi: 10

    Insert

    POT family transporter
    

    Use

    Tags

    C terminal TEV GFP octa-His affinity tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFlag-CMV2-WBP2

    Plasmid

    #27478

    Depositor

    Marius Sudol

    Article

    Chen et al J Biol Chem. 1997 Jul 4. 272(27):17070-7

    Insert

    WW domain binding protein-2 (WBP2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

28. MDO1-c014

    Plasmid

    #173103

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    MACROD1 (MACROD1 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    S58-A325, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET26_MraYaa

    Plasmid

    #100166

    Purpose

    recombinant expression of target protein as an MBP-His tag fusionat the N-terminus of target protein

    Depositor

    Seok-Yong Lee

    Article

    Chung et al Science. 2013 Aug 30;341(6149):1012-1016

    Insert

    mraY (phospho-MurNAc-pentapeptide translocase)
    

    Use

    Tags

    MBP-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28a-mCherry-2xEcMTS

    Plasmid

    #133624

    Purpose

    Expression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

    Insert

    mCherry-2xEcMTS
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge