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TESK2 gRNA (BRDN0001149439)

Plasmid

#76435

Purpose

3rd generation lentiviral gRNA plasmid targeting human TESK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TESK2 (TESK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIM2 gRNA (BRDN0001149489)

Plasmid

#76384

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIM2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIM2 (PIM2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIM2 gRNA (BRDN0001147820)

Plasmid

#76385

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIM2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIM2 (PIM2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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STK25 gRNA (BRDN0001149116)

Plasmid

#75957

Purpose

3rd generation lentiviral gRNA plasmid targeting human STK25

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

STK25 (STK25 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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STK25 gRNA (BRDN0001148478)

Plasmid

#75958

Purpose

3rd generation lentiviral gRNA plasmid targeting human STK25

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

STK25 (STK25 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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STK25 gRNA (BRDN0001146374)

Plasmid

#75959

Purpose

3rd generation lentiviral gRNA plasmid targeting human STK25

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

STK25 (STK25 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pET-DEST42-ARL1-V5-His6

Plasmid

#67416

Purpose

Expression of hArl1 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL1 (ARL1 Human)

Tags

His and V5

Expression

Bacterial

Mutation

bp 231 C to T; silent mutation

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pSEW-Venus-Sec16A-deltaN

Plasmid

#66605

Purpose

Lentiviral vector for express truncated form of Sec16A as a fusion with Venus FP

Depositor

David Stephens

Article

Hughes et al J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8

Insert

Sec16A (SEC16A Human)

Tags

Venus (EYFP derivative)

Expression

Mammalian

Mutation

encodes amino acids 1107-1217 of human Sec16A

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
CS2+MYC-Etv5:T139D

Plasmid

#49097

Purpose

expression of Etv5 T139D mutant

Depositor

Michael Tsang

Article

Znosko et al Dev Biol. 2010 Jun 1;342(1):11-25. Epub

Insert

Etv5 T139D (etv5a Zebrafish)

Use

Xenopus/avian/zebrafish

Tags

MYC

Expression

Mammalian

Mutation

T139D

Promoter

CMV

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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Cs2+Etv5 S142D

Plasmid

#49096

Purpose

expression of Etv5 S142D mutant

Depositor

Michael Tsang

Article

Znosko et al Dev Biol. 2010 Jun 1;342(1):11-25. Epub

Insert

Etv5 S142 D (etv5a Zebrafish)

Use

Xenopus/avian/zebrafish

Expression

Mammalian

Mutation

S142D

Promoter

CMV

Available Since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-YAP-H122Y

Plasmid

#33085

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

H122Y

Promoter

CMV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pCDF1-ADAMTS18 (P1035S)

Plasmid

#29558

Depositor

Yardena Samuels

Article

Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

Insert

ADAMTS18 (ADAMTS18 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed proline 1035 to serine

Available Since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only
pCDF1-ADAMTS18 (C638S)

Plasmid

#29555

Depositor

Yardena Samuels

Article

Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

Insert

ADAMTS18 (ADAMTS18 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed cysteine 638 to serine

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA4/HisMaxB-YAP1-S347A

Plasmid

#18993

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein S347A mutant (YAP1 Human)

Tags

His

Expression

Mammalian

Mutation

Serine 347 changed to Alanine

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
QSOX1-bio-His

Plasmid

#51825

Purpose

Expresses full-length Sulfhydryl oxidase 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

QSOX1 (QSOX1 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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hnRNPA1-FLAG-IRES-eGFP

Plasmid

#234619

Purpose

Mammalian expression of full-length hnRNPA1 with C-terminal FLAG tag co-expressing with eGFP via IRES sequence

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1 (HNRNPA1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

C-terminal FLAG tag, co-expressing with eGFP via …

Promoter

CMV

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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EPHA3_1-972 pcDNA3.1

Plasmid

#102739

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Tags

myc-6xhis

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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TetON-shYAP

Plasmid

#115667

Purpose

Inducible YAP knockdown

Depositor

Joan Massague

Article

Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

Insert

YAP shRNA (YAP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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ppyCAG_RNaseH1_D210N

Plasmid

#111904

Purpose

Express D210N mutated RNASEH1 which abolishes the catalytic activity of RNASEH1

Depositor

Xiang-Dong Fu

Article

Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

Insert

RNASEH1 (RNASEH1 Human)

Tags

V5

Expression

Mammalian

Mutation

Changed D 210 to N

Available Since

July 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCW-myc-YAP5SA-puro

Plasmid

#249725

Purpose

Doxycycline-inducible expression of myc epitope-tagged YAP5SA with puro selection

Depositor

Tobias Meyer

Article

Ferrick et al bioRxiv [Preprint]. 2024 Nov 4:2024.10.1

Insert

YAP5SA (YAP1 Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

S61A, S109A, S127A, S164A, S381A (phosphorylation…

Promoter

Tet ON

Available Since

Feb. 9, 2026

Availability

Academic Institutions and Nonprofits only

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