We narrowed to 7,531 results for: Ski

1. pHS1517

   Plasmid

   #205980

   Purpose

   FLAG-SV40 NLS-Cas5-HNH from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas5-HNH
   

   Tags

   FLAG-SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNAS1b split mCherry 14-3-3β ctrl

   Plasmid

   #112031

   Purpose

   Mode #2 phosphosite control (split mCherry, using 14-3-3β binding domain paired with phospho-dependent interacting peptide with TAG codon)

   Depositor

   Jesse Rinehart

   Article

   Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

   Insert

   14-3-3β fused to C-terminal split mCherry, phospho-dependent binding ctrl peptide with TAG fused to N-terminal split mCherry
   

   Tags

   Split mCherry

   Expression

   Bacterial

   Available Since

   July 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. PVT1-Tol2

   Plasmid

   #113381

   Purpose

   evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

   Depositor

   Ralf Jauch

   Article

   Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

   Insert

   PVT1-enhancer (PVT1 Human)
   

   Use

   Tol2 reporter

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

4. GlycophorinB-CD4d3+4-bio

   Plasmid

   #73094

   Purpose

   EXPRESs plasmid for human erythrocyte surface proteins encoding GlycophorinB with rat CD4d3+4-bio

   Depositor

   Gavin Wright

   Article

   Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

   Insert

   Glycophorin B (GYPB Human)
   

   Tags

   biotinylation peptide and ratCD4d3+4

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Semaphorin7A-CD4d3+4-bio

   Plasmid

   #73115

   Purpose

   EXPRESs plasmid for human erythrocyte surface proteins encoding Semaphorin7A with rat CD4d3+4-bio

   Depositor

   Gavin Wright

   Article

   Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

   Insert

   Semaphorin 7A (SEMA7A Human)
   

   Tags

   biotinylation peptide and ratCD4d3+4

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX Tec-SH2

   Plasmid

   #46522

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Tec (TEC Human)
   

   Tags

   AviTag, GST, and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 236-351

   Promoter

   Tac

   Available Since

   Oct. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX Stat6-SH2

   Plasmid

   #46517

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Stat6 (STAT6 Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 510-654

   Promoter

   Tac

   Available Since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. HES7-NLuc-2A-tdTomato

   Plasmid

   #130932

   Purpose

   Donor vector for tagging human HES7 C-terminus with NanoLuc and tdTomato

   Depositor

   James Thomson

   Article

   Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

   Insert

   HES7 (HES7 Human)
   

   Tags

   NanoLuc and tdTomato

   Expression

   Mammalian

   Available Since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA SF-HERC2 F3

   Plasmid

   #55786

   Purpose

   Expresses N-terminal 3xFLAG/STREP tagged HERC2 (1700-2700aa)

   Depositor

   David Chan

   Article

   Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

   Insert

   3xFLAG tagged HERC2 (1700aa-2700aa) (HERC2 Human)
   

   Tags

   3xFLAG and STREP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2014

   Availability

   Academic Institutions and Nonprofits only

10. Neuroplastin-CD4d3+4-bio

    Plasmid

    #73111

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding Neuroplastin with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    Neuroplastin (NPTN Human)
    

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHS1929

    Plasmid

    #205968

    Purpose

    JAGBLH010000220 Cas8-HNH crRNA expression in pCOLADuet-1

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas8-HNH crRNA
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. LENTICRISPR-PGAM1_sgRNA1

    Plasmid

    #201614

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    PGAM1 (PGAM1 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1P shFXN_1

    Plasmid

    #102970

    Purpose

    Suppression of FXN

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shFXN_1 (FXN Human)
    

    Use

    Lentiviral and RNAi

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHS1477

    Plasmid

    #205970

    Purpose

    MWEE01000013 Cas5-HNH operon (E. coli codon optimized) with His14-tagged Cse2 in pET45b(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas5-HNH all proteins, with His-tagged Cas11
    

    Tags

    14xHis on Cas11

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. HA-MBP-TEVpCS-ADGRA3 CTF-FLAG

    Plasmid

    #217680

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRA3 (ADGRA3 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-737

    Available Since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-MBP-TEVpCS-ADGRA2 CTF-FLAG

    Plasmid

    #217679

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRA2 (ADGRA2 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-746

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET51b-His-TEV-HOB_(HaloTag7_oligo_binder)

    Plasmid

    #167268

    Purpose

    Overexpression of the HaloTag7 variant HOB (halo-based oligonucleotide binder) in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    HOB
    

    Tags

    His x10

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSET-pr-mEos3.1 (mEosFP3.1-A69T)

    Plasmid

    #99221

    Purpose

    Bacterial expression of the primed conversion capable protein pr-mEos3.1 (mEos3.1-A69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mEos3.1
    

    Expression

    Bacterial

    Mutation

    A69T for primed conversion applications

    Promoter

    T7

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA pDEST40 Cas9-HMGB1

    Plasmid

    #183195

    Purpose

    Expression clone

    Depositor

    Emma Haapaniemi

    Article

    Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

    Insert

    HMGB1
    

    Use

    CRISPR

    Tags

    Cas9WT

    Expression

    Mammalian

    Mutation

    n/a

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO.1P shFXN_2

    Plasmid

    #102971

    Purpose

    Suppression of FXN

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shFXN_2 (FXN Human)
    

    Use

    Lentiviral and RNAi

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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