165,075 results

1. pLentiCRISPR-tagBFP

   Plasmid

   #75160

   Purpose

   Derived from LentiCRISPR v1 but expresses tagBFP instead of puromycin resistance

   Depositor

   Beat Bornhauser

   Article

   McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   tagBFP

   Expression

   Mammalian

   Available Since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiGuide-mCherry

   Plasmid

   #217005

   Purpose

   lentiviral plasmid for gRNA cloning with selectable mCherry expression

   Depositor

   Deepta Bhattacharya

   Article

   D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Pet28a-His-tag-BS2

   Plasmid

   #220347

   Purpose

   E. coli expression vector to purify BS2

   Depositor

   Bryan Dickinson

   Article

   Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

   Insert

   BS2
   

   Tags

   His tag

   Expression

   Bacterial

   Available Since

   June 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. FU - dio YFP - W

   Plasmid

   #73858

   Purpose

   For expressing YFP in mammalian cells upon Cre co-expression. Can be made into Lentivirus.

   Depositor

   Elly Nedivi

   Article

   Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

   Insert

   eYFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-CD80

   Plasmid

   #223603

   Purpose

   Lentiviral expression of human CD80 in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   CD80 (CD80 Human)
   

   Use

   Lentiviral; mammalian expression

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. GW1CMV-Perceval

   Plasmid

   #21737

   Purpose

   can be used to monitor the ATP:ADP ratio during live-cell imaging

   Depositor

   Gary Yellen

   Article

   Berg et al Nat Methods. 2009 Feb . 6(2):161-6.

   Insert

   Perceval (fluorescent ATP:ADP sensor)
   

   Tags

   5'UTR

   Expression

   Mammalian

   Mutation

   improved Kozak sequence

   Available Since

   Aug. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pD649-HAsp-KIR2DS5-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156674

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   KIR2DS5 (KIR2DS5 Human)
   

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1_3xFlagEzh2 WT

   Plasmid

   #173717

   Purpose

   WT EZH2 expression plasmid with 3XFLAG tag

   Depositor

   Thomas Cech

   Article

   Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

   Insert

   EZH2 (EZH2 Human)
   

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221_EGFP

   Plasmid

   #25899

   Purpose

   Gateway entry vector containing EGFP.

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   EGFP
   

   Use

   Gateway entry vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBABE-neo largeTcDNA

    Plasmid

    #1780

    Purpose

    Retroviral expression of Large T Antigen. Used to create immortalized cell lines.

    Depositor

    Bob Weinberg

    Article

    Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

    Insert

    large T antigen (SV40gp6 simian virus)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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11. VPS13D^Halo

    Plasmid

    #174108

    Purpose

    The insert is a codon optimized DNA sequence encoding VPS13D with an internal Halo tag.

    Depositor

    Pietro De Camilli

    Article

    Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    Codon optimized VPS13D
    

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    With an internal Halo tag in aminoacid position 1…

    Promoter

    CMV

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. STIM1-EGFP

    Plasmid

    #210370

    Purpose

    Expresses STIM1 fused with EGFP in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    STIM1 (STIM1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. GRM1-DuET

    Plasmid

    #213300

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GRM1 (GRM1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOGG001

    Plasmid

    #113978

    Purpose

    Level 0 module. nptII promoter for constitutive gene expression

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    nptII promoter
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. TRX1 flag C32S

    Plasmid

    #21284

    Depositor

    Wang Min

    Article

    Liu et al Circ Res. 2002 Jun 28. 90(12):1259-66.

    Insert

    TRX1 (TXN Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C32S

    Available Since

    July 30, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pADH110

    Plasmid

    #90982

    Purpose

    NAT-marked pSNR52 promoter fragment for use in gRNA expression cassette stitching PCR; for use with pADH119, 139, or 147 to generate target-specific gRNA expression cassette.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Inserts

    NAT 2 of 2
    pSNR52
    

    Use

    CRISPR

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. lenti-NLS-miRFP670nano-miRFP670

    Plasmid

    #216131

    Purpose

    Lentiviral vector for expressing a fusion of miRFP670nano and miRFP670 localizing to the nucleus.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    EF1a-NuclearLocalizationSignal-miRFP670nano-miRFP670-WPRE
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-TRE3G-YAP1-5SA-CMV-mCherry-T2A-Puro

    Plasmid

    #176851

    Purpose

    lentiviral vector for doxycycline-inducible YAP1 5SA constitutively active expression

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    hYAP1-5SA (YAP1 Human)
    

    Use

    Lentiviral

    Mutation

    S61A, S109A, S127A, S164A, S381A (phosphorylation…

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSEVA63-GG

    Plasmid

    #208015

    Purpose

    for cloning homology arms, with GFP as counter selection marker

    Depositor

    Pablo Ivan Nikel

    Article

    Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

    Insert

    msfGFP
    

    Promoter

    14G

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. HDAC1 Flag

    Plasmid

    #13820

    Purpose

    Mammalian expression of human histone deacetylase 1 with flag tag

    Depositor

    Eric Verdin

    Article

    Emiliani et al Proc Natl Acad Sci U S A. 1998 Mar 17. 9

    Insert

    HDAC1 (HDAC1 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Available Since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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21. pcDNA3.1-CSF1R-V5/HIS

    Plasmid

    #201984

    Purpose

    expression of human CSF1R receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    CSF1R receptor tyrosine kinase (CSF1R Human)
    

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. Keratin 8 (pcDNA3)

    Plasmid

    #18063

    Depositor

    M Bishr Omary

    Article

    Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

    Insert

    Keratin 8 (KRT8 Human)
    

    Expression

    Mammalian

    Available Since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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23. CBIG-RORb

    Plasmid

    #48709

    Purpose

    bicistronic expression of RORb and EGFP

    Depositor

    Jeffrey Macklis

    Insert

    RORb (Rorb Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CMV/beta-actin

    Available Since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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24. Lenti-TetON-Sox2

    Plasmid

    #110280

    Purpose

    Doxycycline-inducible overexpression of Sox2

    Depositor

    Trudy Oliver

    Article

    Mollaoglu et al Immunity. 2018 Oct 16;49(4):764-779.e9.

    Insert

    SRY (sex determining region Y)-box 2 (Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    No fusion genes

    Expression

    Mammalian

    Mutation

    Wild type

    Promoter

    TRE

    Available Since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. pLC242-GFP-metap2

    Plasmid

    #100518

    Purpose

    Expresses GFP tagged metap2 in mammalian cells to make lentivirus

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    Metap2 (METAP2 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. qTAG-C-V5-Puro

    Plasmid

    #207730

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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27. TFORF3167

    Plasmid

    #144643

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF512B (ZNF512B Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. GFP-BAZ1B

    Plasmid

    #65372

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    BAZ1B (BAZ1B Human)
    

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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29. Fusion of Zika NS2B-NS3 proteins

    Plasmid

    #204815

    Purpose

    Bacterial expression of a fusion of Zika NS2B-NS3 proteins

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Zika NS2B-NS3 fusion (orf1ab Zika)
    

    Expression

    Bacterial

    Available Since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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30. LentiGuide Puro-P2A-EGFP

    Plasmid

    #137729

    Purpose

    For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).

    Depositor

    Fredrik Wermeling

    Article

    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

    Type

    Empty backbone

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    EGFP

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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