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CIRC-V1-Ana-CVB3 IRES-EGFP-BbsI

Plasmid

#246754

Purpose

RNA circularization with CIRC using Ana intron, harbouring an CVB3 IRES-EGFP reporter.Plasmid was linearized with BbsI.

Depositor

Wensheng Wei

Article

Shen et al Nat Commun. 2025 Aug 10;16(1):7376. doi:

Insert

CIRC-V1-Ana-CVB3 IRES-EGFP-BbsI

Use

Synthetic Biology

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF0282

Plasmid

#141561

Purpose

Lentiviral vector for overexpressing the MEF2C transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MEF2C (MEF2C Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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σ2-mCherry

Plasmid

#186579

Purpose

Expression of adaptor protein 2 (AP2) sigma subunit AP2S1 tagged with mCherry

Depositor

Stephen Royle

Article

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Insert

AP2S1 (AP2S1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mhYFP

Plasmid

#186533

Purpose

Monomeric hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-No-HaloTag7-mGFP-LC3-mRFP

Plasmid

#184902

Purpose

Stable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Tags

HaloTag7, monomeric EGFP, and monomeric RFP

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO SwiChRca-TS-EYFP

Plasmid

#55631

Purpose

Cre-Dependent Chloride Channel

Depositor

Karl Deisseroth

Article

Berndt et al Science. 2014 Apr 25;344(6182):420-4. do

Insert

iC1C2-TS-EYFP

Use

AAV and Cre/Lox

Tags

EYFP

Expression

Mammalian

Mutation

C128A

Promoter

Ef1a

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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V5-LOV-Turbo-ERM

Plasmid

#199664

Purpose

expresses LOV-Turbo on the mammalian ER membrane, lentiviral vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

Lentiviral

Tags

SEC61B and V5

Expression

Mammalian

Promoter

TRE3G

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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U6-5' MS2-CircRNA

Plasmid

#213750

Purpose

For circular RNA-mediated prime editor using U6-5' MS2-CircRNA in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

5' ribozyme, 5' ligation sequences, 5' MS2, 3' ligation sequences, 3' ribozyme

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJRH-1346 U6-B2M sgRNA Gag-pol v2

Plasmid

#201917

Purpose

Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Inserts

Gag-pol
B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)

Expression

Mammalian

Promoter

CAG and Human U6

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pX330.puro

Plasmid

#110403

Purpose

CRISPR/Cas9 vector with sgRNA expression and puromycin resistance

Depositor

Sandra Martha Gomes Dias

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CBh

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pHCMV-SS-antiFLAGscFv-TM

Plasmid

#198303

Purpose

Displays anti-FLAG scFv from the surface of mammalian cells

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

pHCMV-SS-antiFLAGscFv-TM

Expression

Mammalian

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pJMP1

Plasmid

#79873

Purpose

Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA

Depositor

Carol Gross, Jason Peters, Stanley Qi

Article

Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

Insert

dCas9

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

xylA

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ1651-sgTelomere(F+E)

Plasmid

#51024

Purpose

Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting human telomeres

Depositor

Bo Huang, Stanley Qi

Article

Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

Insert

Optimized sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6 promoter

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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EFS-GFP

Plasmid

#110834

Purpose

positive control for GFP expression

Depositor

Michael Lewis

Article

Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

Insert

elongation factor 1alpha binding sequence (EFS) at GFP promoter region

Use

Lentiviral

Expression

Mammalian

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCaggs-mGold2s

Plasmid

#231763

Purpose

Expression of mGold2s in mammalian cells

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s

Expression

Mammalian

Mutation

mGold2s is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

Promoter

Actin promoter with CMV enhancer

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pSL1119 (pDonor)

Plasmid

#160732

Purpose

Mini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST donor DNA (Mini-Tn)

Expression

Bacterial

Promoter

N/A

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2

Plasmid

#81041

Purpose

Mammalian or insect expression for wt LOV2

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV2

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV, p10

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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M23: pHR-SFFVp-FUSN::mCherry::SspB

Plasmid

#122148

Purpose

N terminal domain of FUS (1-214) tagged with SspB and mCherry.

Depositor

Clifford Brangwynne

Article

Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

Insert

FUS (FUS Human)

Use

Lentiviral

Tags

mCherry-sspB

Expression

Mammalian

Mutation

Contains only amino acids 1-214

Promoter

SFFV

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pTW334e

Plasmid

#237315

Purpose

expresses A. thaliana Cpn60α Cpn60β Cpn20 Raf1 Raf2 RbcX Bsd2

Depositor

Tina Wang

Article

Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

Insert

A. thaliana Cpn60α Cpn60β Cpn20 Raf1 Raf2 RbcX Bsd2

Expression

Bacterial

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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HA-mRIN2

Plasmid

#108940

Purpose

Mammalian expression of mRIN2

Depositor

Guido Serini

Article

Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

Insert

Ras and Rab interactor 2 (Rin2 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pJT305 Pa01 Lenti pEF-attB LSR GFP

Plasmid

#184948

Purpose

Lentiviral delivery of recombinase landing pad

Depositor

Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Pa01 Lenti pEF1a-attB LSR GFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-MMLVgag-dCas9-ZIM3

Plasmid

#240527

Purpose

Expresses MMLVgag–dCas9-ZIM3 for producing RENDER-dCas9-ZIM3

Depositor

James Nuñez

Article

Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

Insert

MMLVgag-dCas9-ZIM3

Tags

FLAG, HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-20nmEML-mRFP

Plasmid

#231181

Purpose

Expresses a synthetic linker, tagged with mRFP, stabilizing the ER-mitochondrial distance at 20 nm from a lentiviral vector

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

20nmEML-mRFP

Use

Lentiviral

Promoter

CMV

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19/PLIN2 GFP-P2A-BFP

Plasmid

#202195

Purpose

knock-in donor vector of GFP-P2A-BFP to C-terminus of endogenous human PLIN2

Depositor

James Olzmann

Article

Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

Insert

PLIN2 (PLIN2 Human)

Use

Cloning vector

Tags

GFP-P2A-BFP knock-in

Promoter

lac

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pGTQL1211YN

Plasmid

#61704

Purpose

Gateway compatible BiFC vector (pEarleyGate201-YN)

Depositor

Yuhai Cui

Article

Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

Type

Empty backbone

Use

Bifc assay

Tags

YFP-N and HA

Expression

Plant

Promoter

35S

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pME-secAnxaV-NS

Plasmid

#67718

Purpose

Gateway entry clone containing secreted human AnxaV with no stop codon

Depositor

Rob Parton

Article

Morsch et al Front Cell Neurosci. 2015 Aug 31;9:321.

Insert

secAnxaV-NS

Expression

Mammalian

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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PLIN2-V5-APEX2

Plasmid

#170573

Purpose

A C-terminal APEX2 fusion to the mouse perilipin-2 protein PLIN2

Depositor

James Olzmann

Article

Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

Insert

PLIN2 (Plin2 Mouse)

Use

Lentiviral

Tags

V5-APEX2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-NPC1

Plasmid

#132295

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

NPC1 (NPC1 Human)

Expression

Mammalian

Available Since

Nov. 14, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pGTQL1221YC

Plasmid

#61705

Purpose

Gateway compatible BiFC vector (pEarleyGate202-YC)

Depositor

Yuhai Cui

Article

Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

Type

Empty backbone

Use

Bifc assay

Tags

YFP-C and FLAG

Expression

Plant

Promoter

35S

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ265E9

Plasmid

#213472

Purpose

Gateway entry clone (attL1 & attR5) TadDE-32aa linker-zSpCas9-D10A-2xUGI for C-T and A to G base editing

Depositor

Yiping Qi

Article

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Insert

TadDE-32aa linker-zSpCas9-D10A-2xUGI

Use

CRISPR; Gateway compatible tadde-32aa linker-zspc…

Tags

NO

Expression

Plant

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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