We narrowed to 16,985 results for: She

1. NanoBRET Assay Vector, HaloTag-KRAS

   Plasmid

   #236871

   Purpose

   Express HaloTag(R)-KRAS in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   KRAS (KRAS Human)
   

   Use

   Tags

   HaloTag (R)

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. NanoBRET Assay Vector, NanoLuc-NRAS(Q61R)

   Plasmid

   #236863

   Purpose

   Express NanoLuc(R)-NRAS Q61R in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   NRAS (NRAS Human)
   

   Use

   Tags

   NanoLuc (R)

   Expression

   Mammalian

   Mutation

   Q61R

   Promoter

   CMV

   Available since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. NanoBRET Assay Vector, NanoLuc-NRAS(Q61K)

   Plasmid

   #236854

   Purpose

   Express NanoLuc(R)-NRAS(Q61K) in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   NRAS (NRAS Human)
   

   Use

   Tags

   HaloTag (R) and NanoLuc (R)

   Expression

   Mammalian

   Mutation

   Q61K

   Promoter

   CMV

   Available since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. NanoBRET Assay Vector, HaloTag-KRAS(G13D)

   Plasmid

   #236856

   Purpose

   Express HaloTag(R)-KRAS(G13D) in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   KRAS (KRAS Human)
   

   Use

   Tags

   HaloTag (R)

   Expression

   Mammalian

   Mutation

   G13D

   Promoter

   CMV

   Available since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. SmBiT-KRAS4B(G12D) BiBiT-Ready Vector

   Plasmid

   #236798

   Purpose

   Express SmBiT-KRAS4B(G12D) Fusion Protein in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   KRAS4B(G12D) (KRAS Human)
   

   Use

   Tags

   SmBiT

   Expression

   Mammalian

   Mutation

   G12D

   Promoter

   CMV

   Available since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pA-CBh.TP-CNOT7-V5H6.MCh.Puro

   Plasmid

   #209946

   Purpose

   In mammalian cells expresses TP fused to a CNOT7 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.

   Depositor

   Kevin Weeks

   Inserts

   CCR4-NOT transcription complex subunit 7 (CNOT7 Human)
   mCherry fluorescent protein fused to puromycin resistance marker
   

   Use

   Tags

   TP (MS2 coat protein) and V5 epitope with H6 tag

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and CMV

   Available since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pA-CBh.TP-CNOT8-V5H6.MCh.Puro

   Plasmid

   #209947

   Purpose

   In mammalian cells expresses TP fused to a CNOT8 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.

   Depositor

   Kevin Weeks

   Inserts

   CCR4-NOT transcription complex subunit 8 (CNOT8 Human)
   mCherry fluorescent protein fused to puromycin resistance marker
   

   Use

   Tags

   TP (MS2 coat protein) and V5 epitope with H6 tag

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and CMV

   Available since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pA-CBh.TP-(inactive)CNOT6-V5H6.MCh.Puro

   Plasmid

   #209943

   Purpose

   In mammalian cells expresses TP fused to an inactive CNOT6 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.

   Depositor

   Kevin Weeks

   Inserts

   CCR4-NOT transcription complex subunit 6 (enzymatically inactive) (CNOT6 Human)
   mCherry fluorescent protein fused to puromycin resistance marker
   

   Use

   Tags

   TP (MS2 coat protein) and V5 epitope with H6 tag

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and CMV

   Available since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSG297-(Sp-gRNA)-(Sa-gRNA)-(PuroR_T2A_BFP(C>T screening))

   Plasmid

   #239448

   Purpose

   Orthogonal dual Cas9 gRNA and BFP reporter lentivirus cassette plasmid. For use with S. pyogenes and S. aureus gRNAs and includes a BFP reporter which reports on C-to-T editing.

   Depositor

   Alexis Komor

   Inserts

   S.p. gRNA backbone
   S.a. gRNA backbone
   PuroR-T2A-BFP(C>T_screening)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   BFP: T65S, S72S, V145F, K206K, H231L

   Promoter

   EF1alpha, U6, and mU6

   Available since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ADEPT-pTarget-GFP

    Plasmid

    #238041

    Purpose

    Encodes sfGFP under lac promoter. Expresses a single guide RNA (under Rha promoter), which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance.

    Depositor

    Lingchong You

    Article

    Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    lac

    Available since

    June 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ADEPT-pTarget-RFP

    Plasmid

    #238040

    Purpose

    Encodes mRFP1 under pLux promoter. Expresses a single guide RNA (under Ara promoter), which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance.

    Depositor

    Lingchong You

    Article

    Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

    Insert

    mRFP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Lux

    Available since

    June 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. eGFP-MYO5C

    Plasmid

    #239278

    Purpose

    Expression of N terminal GFP tagged Myo5C

    Depositor

    David Pellman

    Article

    Zhao et al Dev Cell. 2023 May 22;58(10):847-865.e10

    Insert

    MYO5C (MYO5C Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMIIS794

    Plasmid

    #238207

    Purpose

    Expresses native TaTasR TIGR locus, TasR has premature stop codon. CmR

    Depositor

    Feng Zhang

    Article

    Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

    Insert

    TaTasR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A insertion at K85 codon

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ADEPT-pTarget

    Plasmid

    #238033

    Purpose

    Encodes sfGFP-SsrA under constitutive promoter (J23119). Expresses a single guide RNA, which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance.

    Depositor

    Lingchong You

    Article

    Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    ssrA

    Expression

    Mutation

    Promoter

    J23119 (constitutive)

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLD-puro-Cc-IFIT5_K206R-VA

    Plasmid

    #191224

    Purpose

    Lentiviral expression of human IFIT5_K206R in mammalian cells

    Depositor

    Mohan Babu

    Insert

    interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)
    

    Use

    Lentiviral

    Tags

    3xFlag-TEVx2-6xHis-Strep x2 tag and VA tag

    Expression

    Mammalian

    Mutation

    changed Lysine 206 to Arginine (K206R)

    Promoter

    CMV

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMXs-mBrn2-P2A-mRuby2

    Plasmid

    #233156

    Purpose

    Retroviral expression of fluorescent mRuby2 reporter

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

    Insert

    Brn2-2A-mRuby2 (Pou3f2 Mouse, Synthetic)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28-NFHΔ36-72

    Plasmid

    #232060

    Purpose

    Expresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 36-72

    Depositor

    Sanjay Kumar

    Article

    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

    Insert

    Neurofilament-Heavy tail domain lacking residues 36-72
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    added N-terminal tryptophan and tyrosine, and cys…

    Promoter

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28-NFHΔ462-638

    Plasmid

    #232061

    Purpose

    Expresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 462-638

    Depositor

    Sanjay Kumar

    Article

    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

    Insert

    Neurofilament-Heavy tail domain lacking residues 462-638
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    added N-terminal tryptophan and tyrosine, and cys…

    Promoter

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28-NFLtail

    Plasmid

    #232055

    Purpose

    Expresses construct for surface tethering: R. norvegicus NFL tail domain

    Depositor

    Sanjay Kumar

    Article

    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

    Insert

    Neurofilament-Light tail domain
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    cDNA codon-optimized for E. coli; added N-termina…

    Promoter

    T7

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28-NFMtail

    Plasmid

    #232056

    Purpose

    Expresses construct for surface tethering: R. norvegicus NFM tail domain

    Depositor

    Sanjay Kumar

    Article

    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

    Insert

    Neurofilament-Medium tail domain
    

    Use

    Tags

    6xHis, thrombin

    Expression

    Bacterial

    Mutation

    cDNA codon-optimized for E. coli; added N-termina…

    Promoter

    T7

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28-NFMshuf1

    Plasmid

    #232058

    Purpose

    Expresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 1

    Depositor

    Sanjay Kumar

    Article

    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

    Insert

    Neurofilament-Medium tail domain, charge shuffle 1
    

    Use

    Tags

    6xHis, thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28-NFHtail

    Plasmid

    #232057

    Purpose

    Expresses construct for surface tethering: R. norvegicus NFH tail domain

    Depositor

    Sanjay Kumar

    Article

    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

    Insert

    Neurofilament-Heavy tail domain
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    added N-terminal tryptophan and tyrosine, and cys…

    Promoter

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28-NFMshuf2

    Plasmid

    #232059

    Purpose

    Expresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 2

    Depositor

    Sanjay Kumar

    Article

    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

    Insert

    Neurofilament-Medium tail domain, charge shuffle 2
    

    Use

    Tags

    6xHis, thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. rTetR-GFP-p65

    Plasmid

    #232633

    Purpose

    p65 activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline addition

    Depositor

    Karsten Rippe

    Insert

    rTetR-GFP-p65 (RELA Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pdCas9-GFP-p65

    Plasmid

    #232630

    Purpose

    p65 activation domain coupled to catalytically inactive dCas9 for localization; contains GFP for labeling and as a spacer

    Depositor

    Karsten Rippe

    Insert

    dCas9-GFP-p65 (RELA Human, Synthetic)
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pB-DEST (JDW 1205)

    Plasmid

    #229821

    Purpose

    A PiggyBac destination vector compatible with gateway cloning system.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Kif3A-EGFP-SNAP

    Plasmid

    #229852

    Purpose

    Kif3A (aa1-342)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif3A was used to link an oligo to the motor using the SNAP tag for single molecule experiments

    Depositor

    William Hancock

    Insert

    Kif3A (Kif3a Mouse)
    

    Use

    Tags

    6xHis, EGFP, and SNAP-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLANT-2b

    Plasmid

    #231487

    Purpose

    Expresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.

    Depositor

    Venkat Gopalan

    Inserts

    SUMO^NIT (SMT3 Budding Yeast)
    FraB
    

    Use

    Tags

    His6 tag and His6-SUMO^NIT

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-6 (MEPEES). and M83L, E84Q,…

    Promoter

    T7 promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCRII-TOPO-dgat1a in situ probe

    Plasmid

    #232137

    Purpose

    zebrafish dgat1a anti-sense in situ probe, linearize with NotI, SP6 polymerase

    Depositor

    Steven Farber

    Article

    Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

    Insert

    dgat1a (dgat1a Zebrafish)
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    SP6/T7

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCS2+ HA-BEX1

    Plasmid

    #231002

    Purpose

    Transient overexpression of human BEX1 in mammalian cells

    Depositor

    Michael Rape

    Article

    Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

    Insert

    BEX1 (BEX1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. E547* FL USP7

    Plasmid

    #229001

    Purpose

    E547-mutant of human USP7

    Depositor

    Irina Bezsonova

    Insert

    USP7 1-546 (USP7 Human)
    

    Use

    Tags

    6xHis cleavable with thrombin

    Expression

    Bacterial

    Mutation

    E547 mutated to stop codon

    Promoter

    T7

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1(-)-EGFP-AP4-cyto

    Plasmid

    #229694

    Purpose

    Transient expression of EGFP AP4-cyto in mammalian cells

    Depositor

    Alpha Yap

    Article

    Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

    Insert

    EGFP; Control construct with tandem AP4 motifs (cytoplasm)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3.1(-)-EGFP-FP4-cyto

    Plasmid

    #229695

    Purpose

    Transient expression of FP4-cyto in mammalian cells

    Depositor

    Alpha Yap

    Article

    Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

    Insert

    EGFP, Mitochondira FP4 motifs from Listeria ActA (cytoplasm)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCAGGS-Twin-strep-TEV-DGKδ2

    Plasmid

    #223720

    Purpose

    Express human diacylglycerol kinase delta 2 protein (N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell

    Depositor

    Chiaki Murakami

    Insert

    DGKD (DGKD Human)
    

    Use

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    deleted start codon

    Promoter

    CAG and chicken β-actin promoter

    Available since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCAGGS-PHOSPHO1-TEV-Twin-Strep

    Plasmid

    #222015

    Purpose

    Express human PHOSPHO1 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell

    Depositor

    Chiaki Murakami

    Insert

    PHOSPHO1 (PHOSPHO1, PHOSPHO1 Human)
    

    Use

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    stop codon (TGA) is deleted

    Promoter

    chicken β-actin promoter

    Available since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGG-PIP21

    Plasmid

    #196759

    Purpose

    Contains SOBIR1 promoter

    Depositor

    Marc Somssich

    Article

    Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

    Insert

    SUPPRESSOR OF BIR 1 (SOBIR1 Mustard Weed)
    

    Use

    Greengate (gg) entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLentiCRISPRRv2_sgTom

    Plasmid

    #218346

    Purpose

    CRISPR KO plasmid for tdTomato in human cell lines

    Depositor

    Seth Parker

    Article

    Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

    Insert

    Inserted sgRNA targetting tdTomato
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET26b_AT118-H-V5-His

    Plasmid

    #222850

    Purpose

    E. coli periplasmic expression plasmid encoding Nb. AT118-A, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-H
    

    Use

    Tags

    V5-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. MSCV-TIMP1

    Plasmid

    #186979

    Purpose

    Expresses mouse TIMP1 in mammalian cells

    Depositor

    Yong-Rui Zou

    Article

    Song et al J Exp Med. 2023 Dec 4;220(12):e20230018.

    Insert

    tissue inhibitor of metalloproteinase 1 (Timp1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDGB3_alpha2_BeYDV geminino 2.0 (no ATG)-eGFP (GB5178)

    Plasmid

    #225443

    Purpose

    Geminino 2.0-no ATG on CDS. BeYDV LIR1 + 2nd half intron ICON MP + eGFP w/o ATG + t35S:SIR + P35s + 1st half of intron from ICON MP + BeYDV LIR1.

    Depositor

    Diego Orzaez

    Insert

    BeYDV LIR1 + 2nd half intron ICON MP + eGFP w/o ATG + t35S:SIR + P35s + 1st half of intron from ICON MP + BeYDV LIR1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pET26b_AT118-A-V5-His

    Plasmid

    #222849

    Purpose

    E. coli periplasmic expression plasmid encoding Nb. AT118-A, a nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-A
    

    Use

    Tags

    V5-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. AAV-HGM (optimized)

    Plasmid

    #220988

    Purpose

    Adeno-associated viral vector to express HaloTag-GFP-mito fusion, a cytosolic-localized Halotag-GFP fusion construct

    Depositor

    Joshua Kritzer

    Article

    Deprey et al ACS Chem Biol. 2022 Jan 15. doi: 10.1021

    Insert

    HaloTag-GFP-mito
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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43. pAAV hSyn RSET-T-GECO1.WPRE

    Plasmid

    #211902

    Purpose

    Expresses T-GECO1 in mammalian cells

    Depositor

    Robert Campbell

    Article

    Aggarwal et al Neurophotonics. 2024 Apr;11(2):024207. d

    Insert

    T-GECO1
    

    Use

    AAV

    Tags

    RSET peptide

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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44. hU6-DR_BsmBI_DR-EFS-RfxCas13d-NLS-2A-Puro-WPRE CasRx pre-gRNA backbone

    Plasmid

    #219823

    Purpose

    EF1α-driven expression of RfxCas13d in mammalian cells. For cloning of pre-guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. (Adapted from plasmid #138147)

    Depositor

    Philipp Maass

    Insert

    RfxCas13d
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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45. SUMO CRYGS 16-177 Q16C

    Plasmid

    #217673

    Purpose

    N-terminally truncated human gamma S crystallin residues 16-177 with Q16C mutation for native chemical ligation

    Depositor

    Larry David

    Insert

    CRYGS (CRYGS Human)
    

    Use

    Tags

    6XHis and SUMO

    Expression

    Bacterial

    Mutation

    Glutamine 16 changed to Cysteine, and amino acids…

    Promoter

    T7

    Available since

    May 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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46. pMamCol1

    Plasmid

    #215184

    Purpose

    Allows for stable expression of recombinant (tagged/untagged) proteins in AAVS1 locus of Human cells and transient Protein Expression in E. coli

    Depositor

    Krishna Kishore Inampudi

    Insert

    tsPurple
    

    Use

    AAV, CRISPR, and Synthetic Biology ; Recombinant …

    Tags

    SNAC-StrepII tag

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TetON 3G Bidirectional for Mammalian and Trc with…

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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47. pAAV/CMV-SCLM

    Plasmid

    #216758

    Purpose

    AAV2 transfer vector with the CMV promoter for ubiquitous expression of SuperClomeleon

    Depositor

    George Augustine

    Insert

    SuperClomeleon followed by WPRE and human growth hormone polyA terminator
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S30R and 11 AA deletion in the Cerulean CFP moiet…

    Promoter

    CMV (cytomegalovirus)

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. rSirt3 shRNA (pLKO.1 vector)

    Plasmid

    #216807

    Purpose

    shRNA against rat Sirt3

    Depositor

    Ghazaleh Ashrafi

    Article

    Tiwari et al J Cell Biol. 2024 Jan 1;223(1):e20230504

    Insert

    Sirt3 (Sirt3 Rat)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. GAL4(1-147)

    Plasmid

    #195029

    Purpose

    The control vector GAL4 (1-147) was generated by introducing a stop codon immediately after the GAL4 (1-147) sequence in the GAL4-Creb5 (1-128) vector

    Depositor

    Andrew Lassar, Cheng-Hai Zhang

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    GAL4 (1-147)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. GL4.10 Prg4 promoter-luciferase

    Plasmid

    #195034

    Purpose

    Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

    Depositor

    Andrew Lassar, Cheng-Hai Zhang

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    Prg4 promoter (PRG4 Bovine)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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